• iPS세포에서 유래된 human Cardiomyocytes는 cariotoxicity 테스트, 약물검사, 약물 유효성 확인 및 신진대사 연구, electrophysiology에 사용됩니다.
• 해동되면 in vitro에서 자발적으로 박동하기 시작하며, troponin을 포함한 심장 특이 마커를 발현합니다. 
• Cardiac dysfunction (심부전) 및 cardiotoxicity (심근독성) 연구를 위한 drug testing에도 사용할 수 있습니다.
• Primary cell과 동등한 수준의 결과를 제공합니다.
• 최적의 전용 media를 함께 제공합니다.

Cat. No.	Product Name	Quantity
ax2502	Human iPSC-Derived Ventricular Cardiomyocytes (Female)	1 million cells
ax2505	Human iPSC-Derived Ventricular Cardiomyocytes (Male)	1 million cells
ax2520	Human iPSC-Derived Ventricular Cardiomyocytes (Male)	1 million cells
ax2500	Human iPSC-Derived Ventricular Cardiomyocyte Kit (Male)	1 kit (1 million cells)
ax2515	Human iPSC-Derived Atrial Cardiomyocytes (Male)	1 million cells
ax2530-500	Cardiomyocyte Maintenance Medium	500 mL
ax0044	Unlock	25 mL
ax0049	Fibronectin Coating Solution	1 mL

 

• Immunohistochemistry : Cardiomyocyte 특이적인 marker를 발현합니다.

        Axol Human iPSC-Derived Ventricular Cardiomyocytes were cultured in Cardiomyocyte Maintenance Medium prior
        to being fixed and stained for the markers Sarcomeric Alpha Actinin and Cardiac Troponin. The cells were 
        counterstained using DAPI to allow visualization of nuclei.


       Expression of Troponin T, atrial natriuretic peptide (ANP) and atrial myosin light chain 2 (MLC2a) was confirmed in
       Axol iPSC-Derived Atrial Cardiomyocytes using immunocytochemistry. Troponin T staining (red) confirmed the
       presence of cardiac myocytes which are responsible for sarcomere contraction. ANP is specifically secreted by
       atrial myocytes upon atrial stretching and MLC2a facilitates cardiac contractility wo. DAPI counterstain. 

• Beating : 배양 후 자발적으로 박동합니다.

 

• Action potential characteristics : patch clamp에서 자발적인 action potential을 보여줍니다.

      Characteristics of spontaneous and evoked action potentials recorded from Axol’s Human iPSC-derived Ventricular
      Cardiomyocytes. Spontaneous (A) and evoked (1 Hz; B) action potentials recorded by whole-cell patch clamp.
      (C) Average action potential parameters for spontaneous and evoked action potentials in control conditions.

• Core cardiac ion channel pharmacology : INa, ICa,L and IKr을 발현합니다.

       Axol’s Human iPSC-derived Ventricular Cardiomyocytes express the core cardiac ion channels INa, ICa,L and IKr.
      (A) Representative traces of evoked action potentials recorded under control conditions (grey) and in the presence
      of 100 μM Lidocaine (green), 100 nM Nifedipine (blue) or 50 nM Dofetilide (red), which show expected effects on
      action potential amplitude and duration. B) Average effect of each compound on spontaneous action potential
      parameters, presented as percent of control ± SEM, N ≥ 4. Statistical significance calculated by a paired
      two-tailed Student’s t-test (* p<0.05, ** p<0.01, *** p<0.001).

• Calcium Imaging : 칼슘이미징 분석에 사용할 수 있습니다.

• Modeling Arrhythmias : 부정맥 분석에 사용할 수 있습니다.

 

           Axol’s Human iPSC-Derived Ventricular Cardiomyocytes were treated with doxorubicin (control, 500 nM, 100 nM
           and 50 nM). There was a dose-dependent response at 13 h and 24 h post-treatment with doxorubicin.
           Arrhythmias and loss of beating amplitude were seen at lower doxorubicin concentration, whereas higher 
           doxorubicin concentrations resulted in tachycardia and ultimately cell death. Data kindly provided by
           Dr J. Maynes, University of Toronto.

 

   Protocols