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Á¦´ëÇ÷, ÀüÇ÷, °ñ¼ö¿¡¼ ¾òÀº Stem Cell (Hematopoietic & Mescenchymal) ¹× Progenitor CellÀÇ Áõ½Äµµ¸¦ ÀüÅëÀûÀÎ Methylcellulose CFU Assay, ATP, Çü±¤, Ä÷¯ÃøÁ¤¹ý µî ¿©·¯°¡Áö ¹æ¹ýÀ¸·Î ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù. |
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Preferred Cell System (HemoGenix) |
Methylcellulose Colony Forming Assay | ||||||||||||||||
ÀüÅëÀûÀÎ ¹Ý°íÇü ¸ÅÆ®¸¯½º¸¦ ÀÌ¿ëÇÏ¿© colony¸¦ Ä«¿îÆÃÇÏ´Â ¸Å´º¾ó ¹æ¹ýÀÔ´Ï´Ù. | ||||||||||||||||
Ư¡ | ||||||||||||||||
- ´Ù¾çÇÑ Species¿¡ Àû¿ëµË´Ï´Ù. (human, primate, horse, sheep, pig, dog, rat, mouse µî 8Á¾¿¡ »ç¿ëµÊ) | ||||||||||||||||
- °í°´ÀÇ flexibility¸¦ À§ÇØ cytokineÀÌ ¾ø´Â ŸÀÔ°ú, »ç¿ëÀÇ Æí¸®¸¦ À§ÇØ È¥ÇÕµÈ MasterMix ŸÀÔÀÌ ÀÖ½À´Ï´Ù. | ||||||||||||||||
- Ÿ»ç¿¡ ºñÇØ »ç¿ë·®ÀÌ Àû½À´Ï´Ù. | ||||||||||||||||
- Á¶Ç÷Àü±¸¼¼Æ÷ÀÇ ºÐÈ¿Í Á¤·®¿¡ »ç¿ëµË´Ï´Ù. | ||||||||||||||||
(7 stem cell & 9 progenitor cell & 3 precursor cell population) | ||||||||||||||||
Á¾·ù | ||||||||||||||||
»ç¿ëÇÏ´Â ¹æ½Ä¿¡ µû¶ó 3°¡Áö·Î Á¦°øµË´Ï´Ù.
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ATP Bioluminescence Assay | |
¼¼Æ÷ÀÇ ATP¸¦ Luminometer·Î ÃøÁ¤ÇÏ´Â ¹æ¹ýÀÔ´Ï´Ù. | |
Ư¡ | |
- Luciferin°ú ¼¼Æ÷ÀÇ ATP¹ÝÀÀ¿¡ ÀÇÇÑ ¹ß»ýÇÏ´Â ºûÀ» Luminometer·Î ÃøÁ¤ÇÏ´Â ¹æ¹ýÀÔ´Ï´Ù. - °¡Àå ¹Î°¨Çϰí Á¤È®ÇÕ´Ï´Ù. |
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- ¼¼Æ÷ÀÇ Viability, Cell number, Cytotoxicity, ProliferationµîÀ» ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù. | |
- Standard, controlÀÌ Á¦°øµÇ¾î ºÐ¼®ÀÇ calibration, standardization, validationÀÌ °¡´ÉÇÕ´Ï´Ù. | |
- Luminescence plate reader°¡ ÀÖÀ¸¸é Æí¸®ÇÏ°Ô ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù. | |
- 10ºÐ¾È¿¡ °á°ú¸¦ È®ÀÎÇÒ ¼ö ÀÖ½À´Ï´Ù. |
Hematopoietic Cell | Mesenchymal Stem Cell (MSC) | Immune Cell (Lymphocyte) |
Other Stem Cells | Hepatocyte | Neuro Cell | |
Research Kits | HALO-96 Research | MSCGlo-96 Research | ImmunoGlo-96 | STEMGlo-96 | HepatoGlo-Tox HT | NeuroGlo-Complete |
¡¡ | ¡¡ | MSCGlo-96 Complete | ImmunoGlo-96 MLC | |||
In vitro toxicity Kits | HALO-Tox HT | MSCGlo-Tox HT | ImmunoGlo-Tox HT | |||
Cellular therapy Kits | HALO-96 PCAEQ | MSCGlo-96 HuQC | ||||
¡¡ | STEMpredict | MSCGlo-96 PQR | ||||
¡¡ | HALO-96 SPC-QC | |||||
¡¡ | HALO-96 PQR | |||||
¡¡ | HALO-96 TE | |||||
¡¡ | HALO-96 PMT |
PCAEQ | Progenitor Cell Assay Equivalent |
SPC-QC | Stem and Progenitor Cell - Quality Control Assay |
PQR | Potency, Quality and Release Assay |
TE | Time to Engraftment Assay |
PMT | Patient Monitoring after Transplantation Assay |
Tox HT | High Throughput Hemotoxicity Assay |
PRT | Predictive Residual Toxicity Assay |
Fluorescence Assay | |
¼¼Æ÷°¡ GF-AFC¸¦ ºÐÇØÇÏ¸é¼ ¹ß»ýÇÏ´Â Çü±¤À» Fluorescence reader·Î ÃøÁ¤ÇÏ´Â ¹æ¹ýÀÔ´Ï´Ù. | |
Ư¡ | |
- GF-AFC substrate¿Í ¼¼Æ÷ÀÇ protease°¡ ¹ÝÀÀÇÏ¿© ¹ß»ýÇÏ´Â Çü±¤À» Fluorescence reader·Î ÃøÁ¤ÇÏ´Â ¹æ¹ýÀÔ´Ï´Ù. | |
- ¼¼Æ÷ÀÇ Viability, Cell number, Cytotoxicity, ProliferationµîÀ» ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù. | |
- ex. 380-400nm em. 500nm¿¡¼ ÃøÁ¤ÇÕ´Ï´Ù. | |
- 30ºÐ-3½Ã°£ Á¤µµ ¼Ò¿äµË´Ï´Ù. |
Hematopoietic Cell | Mesenchymal Stem Cell (MSC) | Immune Cell (Lymphocyte) |
Other Stem Cells | |
Research Kits | HemoFLUOR-96 Research | MSCFluor-96 Research | ImmunoFluor-96 | STEMFluor-96 |
¡¡ | ¡¡ | MSCFluor-96 Complete | ImmunoFluor-96 MLC | |
Cellular therapy Kits | HemoFLUOR-96 PCAEQ | |||
¡¡ | HemoFLUOR-96 TE | |||
¡¡ | HemoFLUOR-96 PMT |
Absorbance/Colorimetric Assay | |
¼¼Æ÷°¡ MTS Tetrazolium°ú ¹ÝÀÀÇÏ¿© Çü¼ºµÈ »ê¹°À» Absorbance plate reader·Î ÃøÁ¤ÇÏ´Â ¹æ¹ýÀÔ´Ï´Ù. | |
Ư¡ | |
- MTS Tetrazolium reagent¿Í ¼¼Æ÷ÀÇ ¹ÌÅäÄܵ帮¾Æ°¡ ¹ÝÀÀÇÏ¿© Çü¼ºµÈ yellow formazaneÀ» Absorbance reader·Î ÃøÁ¤ÇÏ´Â ¹æ¹ýÀÔ´Ï´Ù. | |
- ¼¼Æ÷ÀÇ Viability, Cell number, Cytotoxicity, ProliferationµîÀ» ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù. | |
- 490nm¿¡¼ ÃøÁ¤ÇÕ´Ï´Ù. | |
- 1-4½Ã°£ Á¤µµ ¼Ò¿äµË´Ï´Ù. |
Hematopoietic Cell | Mesenchymal Stem Cell (MSC) | Immune Cell (Lymphocyte) |
Other Stem Cells | |
Research Kits | HemoLIGHT-96 Research | MSCLight-96 Research | ImmunoLight-96 | STEMLight-96 |
¡¡ | ¡¡ | MSCLight-96 Complete | ImmunoLight-96 MLC | |
Cellular therapy Kits | HemoLIGHT-96 PCAEQ | |||
¡¡ | HemoLIGHT-96 TE | |||
¡¡ | HemoLIGHT-96 PMT |
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