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 제품안내  면역학   Cancer Research  Epigenetics

PTM Antibodies

Post-translational modifications (PTM)은 유전자 조절이나 세포의 스트레스, 노화 및 DNA repair 등과 연관되어 있습니다.

Epigenetic modification의 중요한 요소로는 phosphorylation, acetylation, ubiquitination, methylation등이 있으며, 그 중에서 validation된 Acetyl Lysine Antibody와 Ubiquitin Antibody를 소개합니다.

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특징
  • Acetyl Lysine Antibody: Lysine 잔기의 ε-amino group상의 acetylation에 의해 post-translationally modified된 단백질을 인식합니다.   
  • IP, WB, ChIP, IF 등의 다양한 실험에 적용되며, acetyl lysine antibody에 대한 positive control을 별도로 제공가능합니다. (AACX1, AACX2)
  • Ubiquitin Antibody: 다양한 종의 ubiquitin을 인식하며, Protein G affinity chromatography 방법으로 정제하여 동결건조된 형태로 제공됩니다.
Cat. No. Product Validated applications Species reactivity Host/Isotype Clone
AAC01 Anti-Acetyl Lysine Mouse Mab WB, IF, IP, ChIP All Mouse/IgG2b 3C6.08.20
AUB01 Anti-Ubiquitin Mouse Mab WB, IF Wide range of species Mouse/IgG1 P4D1


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Immunofluorescence using Acetyl Lysine Antibody

Human epidermoid carcinoma A431 cells, untreated (top) or treated (bottom) with TSA (5 mM for 12 h), were stained as    described in the method. Acetylated cytoplasmic and nuclear proteins were visualized in green fluorescence. Note that in contrast with the untreated control, acetylated microtubule network is clearly visible in TSA-treated sample. The fluorescent nuclear  intensities indicate the high abundance of acetylated proteins in the nucleus. 

Western Blot using Ubiquitin Antibody

Anti-Ubiquitin Antibody (Cat. # AUB01) was used at a 1:500 dilution. Bovine thymus ubiquitin was run as follows; Lane 1-50 ng, Lane 2-25 ng. Lane 3– 12.5 ng, Lane 4-6.25 ng, Lane 5-3.12 ng.  Lanes 6 & 7 represent 20 µg of Swiss 3T3 cell lysate  from cells treated for 5h with 10 µM MG132 (Lane 6) or untreated cells (Lane 7). Arrow indicates free ubiquitin band (8 kD), higher molecular weight bands are ubiquitinated proteins.

  


실험논문
  • Bogdan P. and Sherman F. 2002. The diversity of acetylated proteins. Genome Biol. 3 (5): reviews 0006.
  • Lundby A. et al. 2012. Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and cellular patterns. Cell Reports 2:419-431.
  • Sadoul K. et al. 2010. The tale of protein lysine acetylation in the cytoplasm. J. Biomed. Biotech. 2011:1-15.
  • Golemis EA et. Al, Protein-Protein Interactions, CSHLP, 2005, p67.
  • Grabbe, C. et al. 2011. The spatial and temporal organization of ubiquitin net-works. Nat. Rev. Mol. Cell. Biol. 12:295-307.
  • Deshaies R.J. & Joazeiro, C.A. 2009. RING domain E3 ligases. Ann. Rev. Bio-chem. 78: 399-434.
  • Lomeli, H. & Vazquez. 2011. Emerging roles of the SUMO pathway. Cell Mol. Life Sci. 68:4045-4064.
  • Faesen, A.C. et al. 2012. The role of UBL domains in ubiquitin specific proteases. Biochem. Soc. Trans. 40:539-545.
  • Zuin, A. et al. 2014. Ubiquitin signaling: Extreme conservation as a source of diversity. Cells 3:690-701.