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Stable Cell Line Á¦ÀÛ¼­ºñ½º

Target geneÀ» Áö¼ÓÀûÀ¸·Î Overexpression/Knockdown ÇÏ´Â cell lineÀ» Á¦ÀÛÇØ µå¸³´Ï´Ù.

GenScript
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  • Ÿ°Ù À¯ÀüÀÚ¸¦ ¹ßÇöÇÏ´Â stable cell lineÀ» Á¦ÀÛÇØ µå¸³´Ï´Ù.
  • Lentivirus ¹æ¹ý ȤÀº ºñ ¹ÙÀÌ·¯½º ¹æ¹ýÀ» ¼±ÅÃÇÏ½Ç ¼ö ÀÖ½À´Ï´Ù.
  • LentiCRISPR ±â¼úÀº Broad Institute, Inc.ÀÇ ¶óÀ̼±½º¸¦ ¹Þ¾Ò½À´Ï´Ù.
  • membrane proteinÀ» ¹ßÇöÇÏ´Â ´Ù¼öÀÇ ¼¼Æ÷ÁÖ Á¦ÀÛ °æÇèÀ» °¡Áö°í ÀÖ½À´Ï´Ù.
  • mRNA ¹ßÇöÀ» º¸ÁõÇϸç, clone °ËÁõÀ» À§ÇÑ ¿©·¯ ºÐ¼®À» Á¦°øÇÕ´Ï´Ù. (FACS, WB, qPCR, IP µî)

 



¼­ºñ½º¸í Á¦ÀÛ ±â°£ Á¦°ø³»¿ª
Gene overexpression
¼¼Æ÷ÁÖ Á¦ÀÛ
Stable pool : 8ÁÖ
Single clone : 14ÁÖ
• Report of cell line test
• 2 cryovials of stable pool cells with 10^6 cells per vial.
• Report of cell clone generation
• Report of screening of cell clones
• Two single-cell-derived stable clones (1×10^6 cells/vial)
• CoA report
Gene overexpression
¼¼Æ÷ÁÖ Á¦ÀÛ ¹× Functional assays
Stable pool : 16ÁÖ
Single clone : 22ÁÖ
• Report of cell line test
• 2 cryovials of stable pool cells with 10^6 cells per vial.
• Report of cell clone generation
• Report of screening of cell clones
• Report of assay test
• One single-cell-derived stable clone (1×10^6 cells/vial)
• COA report/assay protocol
Inducible systemÀ»
ÀÌ¿ëÇÑ ¼¼Æ÷ÁÖ Á¦ÀÛ
Stable pool : 12ÁÖ
Single clone : 18ÁÖ
• Report of cell line test
• 2 cryovials of stable pool cells with 10^6 cells per vial.
• Report of cell clone generation
• Report of screening of cell clones
• One single-cell-derived stable clone (1×10^6 cells/vial)
• COA report
Single or multiplex genes editing knockout ¼¼Æ÷ÁÖ Á¦ÀÛ 16-24ÁÖ • 1-2 full-allelic knockout cell lines validated by sequencing
• One negative knockout cell line as control
• Biweekly project updates
**Validation of full-allelic knockout cell lines at mRNA or protein level upon request
Single gene knockout stable cell pool Á¦ÀÛ 5-11ÁÖ • Stable cell pool containing knockout clones validated by sequencing
• Biweekly project updates
**Validation of full-allelic knockout cell lines at mRNA or protein level upon request