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 Á¦Ç°¾È³»  ºÐÀÚ»ý¹°ÇР  Viral Expression  Adenoviral Expression

Recombinant expression Adenovirus

Gene delivery vehicle·Î ÀÌ¿ëµÇ´Â premade adenovirus ¸¦ ¼Ò°³ÇÕ´Ï´Ù.

GenTarget
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    • E1 °ú E3 genomic region ÀÌ Á¦°ÅµÈ human adenovirus type5 À¯·¡ virus vector ÀÔ´Ï´Ù.
    • enhanced human cytomegalovirus (suCMV) promoterÇÏ¿¡¼­ ¹ßÇöµË´Ï´Ù.
    • fluorescent marker (GFP or RFP)°¡ ¹ßÇöµË´Ï´Ù.
    • Adenovirus´Â 1 x 108 IFU/ml (200 ul aliquots)À¸·Î Á¦°øµÇ¸ç ¹Ù·Î »ç¿ëÇÒ ¼ö ÀÖ½À´Ï´Ù.
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    GenTarget pre-made expression adenovirus contains a target expressed under enhanced human cytomegalovirus (suCMV) promoter. When applicable, a fluorescent marker (GFP or RFP) is bicistronically expressed under the same promoter, mediated by a 2A element which allows expressing two individual targets. Please see map scheme above for the core expression cassette.

    The pre-made adenovirus¡¯ titers (Ifu/ml) are calculated by the positive cell counts of the fluorescent or other marker after virus transduced into Hela cells. The represented titers are close to the real infectious units, the PFU titers. Adenovirus are provided in 200 ul aliquots as ready-to-use status, either in DMEM medium with 10% FBS (1¡¿108 Ifu/ml), or in PBS solution with 5% sucrose (1¡¿1010 ifu/ml) as for in vivo application.