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 제품안내  분자생물학   Viral Expression  Adenoviral Expression

Recombinant expression Adenovirus

Gene delivery vehicle로 이용되는 premade adenovirus 를 소개합니다.

GenTarget
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    특징
    • E1 과 E3 genomic region 이 제거된 human adenovirus type5 유래 virus vector 입니다.
    • enhanced human cytomegalovirus (suCMV) promoter하에서 발현됩니다.
    • fluorescent marker (GFP or RFP)가 발현됩니다.
    • Adenovirus는 1 x 108 IFU/ml (200 ul aliquots)으로 제공되며 바로 사용할 수 있습니다.
    원리
    GenTarget pre-made expression adenovirus contains a target expressed under enhanced human cytomegalovirus (suCMV) promoter. When applicable, a fluorescent marker (GFP or RFP) is bicistronically expressed under the same promoter, mediated by a 2A element which allows expressing two individual targets. Please see map scheme above for the core expression cassette.

    The pre-made adenovirus’ titers (Ifu/ml) are calculated by the positive cell counts of the fluorescent or other marker after virus transduced into Hela cells. The represented titers are close to the real infectious units, the PFU titers. Adenovirus are provided in 200 ul aliquots as ready-to-use status, either in DMEM medium with 10% FBS (1×108 Ifu/ml), or in PBS solution with 5% sucrose (1×1010 ifu/ml) as for in vivo application.