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 Á¦Ç°¾È³»  ¼¼Æ÷¹è¾ç   Culture Media & Supplement  Contamination Control

Mycoplasma Removal Agent (MRA)

Mycoplasma´Â ¼¼Æ÷ ¹è¾ç ½Ã °¡Àå ÈçÇÏ°Ô ¿À¿°µÇ´Â ¹Ì»ý¹°ÀÇ ÀÏÁ¾À¸·Î, ¿À¿°µÉ °æ¿ì ¼¼Æ÷ Áõ½Ä, ´ë»ç ¹× ºÐÈ­ µî¿¡ ½É°¢ÇÑ ¿µÇâÀ» ¹ÌĨ´Ï´Ù. Mycoplasma Removal Agent (MRA)´Â ¼¼Æ÷ µ¶¼º ¾øÀÌ ´Ù¾çÇÑ mycoplasma¸¦ È¿°úÀûÀ¸·Î Á¦°ÅÇÒ »Ó¸¸ ¾Æ´Ï¶ó ¿À¿° Àç¹ßÀ» ¹æÁöÇØ ÁÝ´Ï´Ù.

MP Biomedicals
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  • Quinolones °è¿­ÀÇ Ç×»ýÁ¦·Î½á, mycoplasmaÀÇ DNA gyrase¸¸À» inhibitionÇÏ¿© ¼¼Æ÷¿¡´Â ÀüÇô ¿µÇâÀ» ÁÖÁö ¾Ê½À´Ï´Ù.
  • Mycoplasma·Î ¿À¿°µÈ ¼¼Æ÷ÀÇ media¿¡ ÷°¡ ÈÄ, ¾à 1ÁÖÀϰ£ÀÇ ¹è¾çÀ¸·Î Á¦°Å°¡ ¿Ï·áµÇ¹Ç·Î »ç¿ëÀÌ °£ÆíÇÏ°í ½Ã°£ÀÌ ´ÜÃàµË´Ï´Ù.
  • ´ëºÎºÐÀÇ Mycoplasma¿¡ Àû¿ëÀÌ °¡´ÉÇÏ¿© º°µµÀÇ Ãß°¡ ½Ã¾àÀÌ ÇÊ¿äÇÏÁö ¾Ê½À´Ï´Ù.
  • Cell culture½Ã ³·Àº ³óµµ·Î MRA¸¦ ¹Ì¸® »ç¿ëÇϸé Àç¿À¿°À» ¿¹¹æÇÒ ¼ö ÀÖ½À´Ï´Ù.
  • Â÷±¤ÇÏ¿© ½Ç¿Â¿¡¼­ º¸°üÇϹǷΠ¿Âµµ¿¡ ÀÇÇÑ Activity º¯È­°¡ ¾ø¾î Ç×»ó ÀÏÁ¤ÇÑ °á°ú¸¦ ¾òÀ» ¼ö ÀÖ½À´Ï´Ù.
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  1. Mycoplasma°¡ ¿À¿°µÈ ¼¼Æ÷¿¡ MRA¸¦ 1/100 ³óµµ·Î ÷°¡Çϰí 1ÁÖÀϰ£ ¹è¾ç½Ãŵ´Ï´Ù. (¿¹, ¹èÁö 10ml ´ç MRA 0.1ml ÷°¡)
  2. Media¸¦ ±³È¯Çϰųª passage ÁøÇà ½Ã¿¡µµ µ¿ÀÏÇÑ ³óµµ·Î MRA°¡ Æ÷ÇÔµÈ ¹èÁö¸¦ »ç¿ëÇÏ½Ã¸é µË´Ï´Ù.
  3. MRA°¡ Æ÷ÇÔµÇÁö ¾ÊÀº media¿¡¼­ ¿©·¯ Â÷·Ê passage¸¦ ´Ã¸° ÈÄ Mycoplasma Hoechst Stain KitÀ» ÀÌ¿ëÇÏ¿© mycoplasma ¿À¿°ÀÌ Àç¹ßµÇÁö ¾ÊÀ½À» È®ÀÎÇÕ´Ï´Ù.
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  1. 1. Data on mycoplasma removal effect - spontaneous infection.
      MRA Tiamulin Minocycline
     MIC*MMC**MICMMCMICMMC
    M. orale CH-19299 0.05 0.1 0.0031 3.13 0.05 25
    M. arginini G-230 0.1 0.2 0.0063 12.5 0.2 >100
    M. hyorhinis BST-7 0.05 0.1 0.0031 0.39 0.0031 0.39
    A. laidlawii PG-8 0.0125 0.025 0.05 >100 0.05 >100
    MMC/MIC2128 ----------> 2048512 ----------> 2048
    + = Mycoplasma positive
    - = Mycoplasma negative
    Duration treated with MRA: 7 days
  2. 2. Efficacy comparison of MRA and pharmaceuticals on the market.
      MRA Tiamulin Minocycline
     MIC*MMC**MICMMCMICMMC
    M. orale CH-19299 0.05 0.1 0.0031 3.13 0.05 25
    M. arginini G-230 0.1 0.2 0.0063 12.5 0.2 >100
    M. hyorhinis BST-7 0.05 0.1 0.0031 0.39 0.0031 0.39
    A. laidlawii PG-8 0.0125 0.025 0.05 >100 0.05 >100
    MMC/MIC2128 ----------> 2048512 ----------> 2048
    M. = Mycoplasma A. = Acholeplasma
    * = Minimum inhibitory concentration (ug/ml) ** = Minimum mycoplasmacidal concentration (ug/ml)
  3. 3. MIC of MRA to other mycoplasma (ug/ml)
    SpeciesMIC
    Mycoplasma fermentans PG-18 0.0125
    Mycoplasma salivarium PG-20 0.1
    Mycoplasma hominis PG-21 0.1
    Mycoplasma buccale CH-20247 0.025


 

ÁÖ¹®Á¤º¸(Ordering Information)
CATNUM PRODUCT SIZE
093050044Mycoplasma Removal Agent (50 ug/ml)5 ml