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 Á¦Ç°¾È³»  Ç×ü,Ç׿ø,ŶƮ   Neuroscience  DNA/RNA Damage

UV Induced DNA Damage Kits

UV light¿¡ ÀÇÇÑ DNA º´º¯ ±¸Á¶ÀÎ CPD ȤÀº (6-4) photoproduct¸¦ ELISA ¶Ç´Â Immunostaining¹æ¹ýÀ» ÀÌ¿ëÇÏ¿© ÃøÁ¤ÇÏ´Â Á¦Ç°ÀÔ´Ï´Ù.

Cell Biolabs
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    • Cellular UV-Induced DNA Damage ELISA Kit
      • Intact cell¿¡¼­ UV¿¡ ÀÇÇÑ DNA ¼Õ»ó °á°úÀÎ CPD ¶Ç´Â 6-4PP¸¦ ELISA ¹æ¹ýÀ¸·Î ÃøÁ¤ÇÕ´Ï´Ù.
      • Microplate reader¸¦ ÀÌ¿ëÇÏ¿© °á°ú¸¦ È®ÀÎÇÕ´Ï´Ù.
      • Kit ±¸¼ºÇ°
        • Anti-6-4PP ȤÀº CPD Antibody, 100X
        • Secondary Antibody, HRP Conjugate
        • Denaturation Solution A, 100X
        • Denaturation Solution B, 100X
        • Assay Diluent
        • 10X Wash Buffer
        • Substrate Solution
        • Stop Solution
    • Cellular UV-Induced DNA Damage Staining Kit
      • Immunostaining ¹æ¹ýÀ» ÀÌ¿ëÇÏ¿© Intact cell¿¡¼­ UV¿¡ ÀÇÇÑ DNA ¼Õ»óÀÎ CPD ¶Ç´Â 6-4PPÀ» ÃøÁ¤ÇÕ´Ï´Ù.
      • ¿°»öµÈ ¼¼Æ÷´Â Çü±¤Çö¹Ì°æÀ» ÅëÇÏ¿© È®ÀÎÇÒ ¼ö ÀÖ½À´Ï´Ù.
      • Kit ±¸¼ºÇ°
        • Anti-6-4PP ȤÀº CPD Antibody, 100X
        • Secondary Antibody, FITC Conjugate, 100X
        • Denaturation Solution A, 100X
        • Denaturation Solution B, 100X
        • Assay Diluent
        • 10X Wash Buffer
    • UV-Induced DNA Damage ELISA Kit
      • ELISA ¹æ¹ýÀ¸·Î Isolated DNA¿¡¼­ CPD ȤÀº 6-4PP¸¦ È®ÀÎÇÏ´Â Kit Á¦Ç°ÀÔ´Ï´Ù.
      • Combo kitÀÇ °æ¿ì 1°³ÀÇ plate¿¡¼­ CPD¿Í 6-4PP¸¦ °¢°¢ 48 well ¾¿ ÀÌ¿ëÇÏ¿© 2°¡Áö ¸ðµÎ¸¦ ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù.
      • Kit ±¸¼ºÇ°
        • 96-well DNA High-Binding Plate
        • Anti-6-4PP/CPD Antibody
        • Secondary Antibody, HRP Conjugate
        • Blocking Reagent (100X)
        • Assay Diluent
        • 10X Wash Buffer
        • Substrate Solution
        • Stop Solution
        • 6-4PP/CPD-DNA Standard
Figure 1. Formation of Pyrimidine Dimers CPD and 6-4PP from Exposure to Ultraviolet Radiation.
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Figure 2. UV-Induced DNA Damage in HeLa Cells as Detected with the OxiSelect™ Cellular UV-Induced DNA Damage ELISA (CPD).
Figure 3. UV-Induced DNA Damage in HeLa Cells Treated with Ultraviolet Light for 30 Minutes and Visualized with the OxiSelect™ Cellular UV-Induced DNA Damage Staining Kit (CPD).