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 Á¦Ç°¾È³»  Ç×ü,Ç׿ø,ŶƮ   Cell Signaling  Reporter Assay

©¬-Galactosidase Staining Kit

À¯ÀüÀÚÀÇ ±â´É ¹× ¹ßÇöÁ¶Àý ¿¬±¸¿¡ »ç¿ëµÇ´Â Reporter geneÀÎ LacZÀÇ ¹ßÇöÀ» °£´ÜÇÑ ¿°»ö¹æ¹ýÀ¸·Î È®ÀÎÇÕ´Ï´Ù.

Cell Biolabs
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  • LacZ geneÀÇ ¹ßÇö°ú transfection È¿À²À» °£ÆíÇÏ°Ô È®ÀÎÇÒ ¼ö ÀÖ½À´Ï´Ù.
  • Transfected geneÀÌ ¹ßÇöµÈ ¼¼Æ÷(lacZ ¹ßÇö)¿¡¼­ ß-Galactosidase¿¡ ÀÇÇØ X-galÀÇ ºÐÇØ°¡ ÀϾ°í, ÀÌ·Î ÀÎÇØ ÆÄ¶õ»öÀ» ¶ç°Ô µË´Ï´Ù.
  • ¿°»ö¹æ¹ýÀÌ °£´ÜÇϸç, 35mm dishes ±âÁØÀ¸·Î 75 Assay¸¦ ÁøÇàÇÒ ¼ö ÀÖ´Â ÃæºÐÇÑ ¾çÀÇ ½Ã¾àÀ» Á¦°øÇÕ´Ï´Ù.
Kit ±¸¼ºÇ°
  • 100X Fixing Solution
  • Staining Solution A
  • Staining Solution B
  • Staining Solution C
  • X-gal Solution
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Figure 1: X-gal staining of infected HUVEC cells and chick CAM tissue. A.
HUVEC cells were infected with purified Ad-β Gal at 50 MOI (multiplicity of infection). X-gal staining was performed after 48 hr infection period. B. Purified Ad- β Gal was injected intravenously into a 10-day old chick embryo, after three days, X-gal staining was performed on the CAM (chick chorioallanoic membrane) tissue.
ÁÖ¹®Á¤º¸(Ordering Information)
CATNUM PRODUCT SIZE
AKR-100¥â-Galactosidase Staining Kit1 kit