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 ǰȳ  ڻ   Viral Expression  Lentiviral Expression

QuickTiter Lentivirus Titer & Quantitation Kits

Lentivirus expression ȿ ϱ lentivirus quantitation ʿմϴ. Traditional p24 ELISA, Lentivirus-associated p24 ELISA, Lentivirus Rapid Quantitation 3 ȳմϴ.

Cell Biolabs
ǰ ϱ

   Lentivirus-Associated p24 ELISA Kit (VPK-107)

  • free p24 virus-associated p24 ϱ p24 ELISA kit lentivirus titer overestimation ּȭ մϴ.
  • Ϲ microplate reader ̿Ͽ ֽϴ.
  • HIV-1 p24 standard ˴ϴ.

Recent Product Citations
1. Narayan, P. et al. (2020). PICALM Rescues Endocytic Defects Caused by the Alzheimer's Disease Risk Factor APOE4. Cell Rep. 33(1):108224. doi: 10.1016/j.celrep.2020.108224.
2. Lyu, P. et al. (2020). Sensitive and reliable evaluation of single-cut sgRNAs to restore dystrophin by a GFP-reporter assay. PLoS One. 15(9):e0239468. doi: 10.1371/journal.pone.0239468.
3. Choi, J.A. Cross-Protection against MERS-CoV by Prime-Boost Vaccination Using Viral Spike DNA and Protein. J Virol. doi: 10.1128/JVI.01176-20.
4. Hoffmann, M.A.G. et al. (2020). Nanoparticles presenting clusters of CD4 expose a universal vulnerability of HIV-1 by mimicking target cells. Proc Natl Acad Sci U S A. doi: 10.1073/pnas.2010320117.
5. Fernandes-Junior, S.A. et al. (2020). Stimulation of Retrotrapezoid Nucleus Phox2b-expressing Neurons Rescues Breathing Dysfunction in an Experimental Parkinson's Disease Rat Model. Brain Pathol. doi: 10.1111/bpa.12868.
6. Folegatti, P.M. et al. (2020). Safety and immunogenicity of a candidate Middle East respiratory syndrome coronavirus viral-vectored vaccine: a dose-escalation, open-label, non-randomised, uncontrolled, phase 1 trial. Lancet Infect Dis. pii: S1473-3099(20)30160-2. doi: 10.1016/S1473-3099(20)30160-2.
7. Purroy, R. et al. (2020). Frataxin-deficient cardiomyocytes present an altered thiol-redox state which targets actin and pyruvate dehydrogenase. Redox Biology. 32:101520. doi: 10.1016/j.redox.2020.101520.
8. Javidi-Parsijani, P. et al. (2020). CRISPR/Cas9 increases mitotic gene conversion in human cells. Gene Ther. doi: 10.1038/s41434-020-0126-z.
9. Yang, H. et al. (2020). Understanding the structural basis of HIV-1 restriction by the full length double-domain APOBEC3G. Nat Commun. 11(1):632. doi: 10.1038/s41467-020-14377-y.
10. Goo, J. et al. (2020). Characterization of novel monoclonal antibodies against MERS-coronavirus spike protein. Virus Res. doi: 10.1016/j.virusres.2020.197863.
11. Czarnek, M. et al. (2019). Proteolytic Processing of Neuregulin 2. Mol Neurobiol. doi: 10.1007/s12035-019-01846-9.
12. Pan, X. et al. (2019). Lysine-specific demethylase-1 regulates fibroblast activation in pulmonary fibrosis via TGF-β1/Smad3 pathway. Pharmacol Res. 152:104592. doi: 10.1016/j.phrs.2019.104592.
13. Bussiere, R. et al. (2019). Upregulation of the Sarco-Endoplasmic Reticulum Calcium ATPase 1 Truncated Isoform Plays a Pathogenic Role in Alzheimer's Disease. Cells. 8(12). pii: E1539. doi: 10.3390/cells8121539.
14. Jha, A. et al. (2019). Alterations in Plasma Membrane Ion Channel Structures Stimulate NLRP3 Inflammasomes Activation in APOL1 Risk Milieu. FEBS J. doi: 10.1111/febs.15133.
15. Zhang, S. et al. (2019). The resistance of esophageal cancer cells to paclitaxel can be reduced by the knockdown of long noncoding RNA DDX11-AS1 through TAF1/TOP2A inhibition. Am J Cancer Res. 9(10):2233-2248.
16. Park, T.Y. et al. (2019). Chloroquine modulates inflammatory autoimmune responses through Nurr1 in autoimmune diseases. Sci Rep. 9(1):15559. doi: 10.1038/s41598-019-52085-w.
17. Gonzalez, S.M. et al. (2019). Vitamin D treatment of peripheral blood mononuclear cells modulated immune activation and reduced susceptibility to HIV-1 infection of CD4+ T lymphocytes. PLoS One. 14(9):e0222878. doi: 10.1371/journal.pone.0222878.
18. Ruscic, J. et al. (2019). Lentiviral Vector Purification Using Nanofiber Ion Exchange Chromatography. Mol Ther Methods Clin Dev. doi: 10.1016/j.omtm.2019.08.007.
19. Poorebrahim, M. et al. (2019). Production of CAR T-cells by GMP-grade lentiviral vectors: Latest advances and future prospects. Crit Rev Clin Lab Sci. doi: 10.1080/10408363.2019.1633512.
20. Blomberg, R. et al. (2019). Fibroblast activation protein restrains adipogenic differentiation and regulates matrix-mediated mTOR signaling. Matrix Biol. pii: S0945-053X(19)30189-1. doi: 10.1016/j.matbio.2019.07.007.
21. Lyu, P. et al. (2019). Delivering Cas9/sgRNA ribonucleoprotein (RNP) by lentiviral capsid-based bionanoparticles for efficient 'hit-and-run' genome editing. Nucleic Acids Res. pii: gkz605. doi: 10.1093/nar/gkz605.
22. Ommer, A. et al. (2019). Ral GTPases in Schwann cells promote radial axonal sorting in the peripheral nervous system. J Cell Biol. pii: jcb.201811150. doi: 10.1083/jcb.201811150.
23. Kumar, R. et al. (2019). The Cytosine Deaminase AICDA Regulates FGF/ERK Signaling to Achieve the Naïve Pluripotent State During Reprogramming. Stem Cells. doi: 10.1002/stem.3023.
24. Zhang, M. et al. (2019). Downregulation of miR143/145 gene cluster expression promotes the aortic media degeneration process via the TGF-β1 signaling pathway. Am J Transl Res. 11(1):370-378.
25. Lee, S.Y. et al. (2019). Optogenetic control of iPS cell-derived neurons in 2D and 3D culture systems using channelrhodopsin-2 expression driven by the synapsin-1 and calcium-calmodulin kinase II promoters. J Tissue Eng Regen Med. 13(3):369-384. doi: 10.1002/term.2786.
26. DaSilva-Arnold, S.C. et al. (2019). ZEB2, a master regulator of the epithelial-mesenchymal transition, mediates trophoblast differentiation. Mol Hum Reprod. 25(2):61-75. doi: 10.1093/molehr/gay053.
27. Mohammadzadeh, N. et al. (2019). Polymorphisms of the cytidine deaminase APOBEC3F have different HIV-1 restriction efficiencies. Virology. 527:21-31. doi: 10.1016/j.virol.2018.11.004.
28. Xie, X. et al. (2019). PPPDE1 promotes hepatocellular carcinoma development by negatively regulate p53 and apoptosis. Apoptosis. 24(1-2):135-144. doi: 10.1007/s10495-018-1491-6.
29. Sanz, R. et al. (2019). In Situ Peroxidase Labeling and Mass Spectrometry of Alpha-Synuclein in Rat Cortical Neurons. Methods Mol Biol. 1948:235-246. doi: 10.1007/978-1-4939-9124-2_18.
30. Lu, B. et al. (2019). Delivering SaCas9 mRNA by lentivirus-like bionanoparticles for transient expression and efficient genome editing. Nucleic Acids Res. doi: 10.1093/nar/gkz093.


   Traditional p24 ELISA Kit (VPK-108)

  • recombinant HIV-1 based lentivirus p24 core protein մϴ.
  • p24 standard ˴ϴ.

Recent Product Citations
1. Saulle, I. et al. (2020). A New ERAP2/Iso3 Isoform Expression Is Triggered by Different Microbial Stimuli in Human Cells. Could It Play a Role in the Modulation of SARS-CoV-2 Infection?. Cells. 9(9):E1951. doi: 10.3390/cells9091951.
2. Miwa, S. et al. (2020). Efficient engraftment of genetically modified cells is necessary to ameliorate central nervous system involvement of murine model of mucopolysaccharidosis type II by hematopoietic stem cell targeted gene therapy. Mol Genet Metab. doi: 10.1016/j.ymgme.2020.06.007.
3. Pereira, M.S. et al. (2019). Loss of SPINT2 expression frequently occurs in glioma, leading to increased growth and invasion via MMP2. Cell Oncol (Dordr). doi: 10.1007/s13402-019-00475-7.
4. Song, J.J. et al. (2018). Cografting astrocytes improves cell therapeutic outcomes in a Parkinson's disease model. J Clin Invest. 128(1):463-482. doi: 10.1172/JCI93924.
5. Luo, J. et al. (2018). CLDN18.1 attenuates malignancy and related signaling pathways of lung adenocarcinoma in vivo and in vitro. Int J Cancer. 143(12):3169-3180. doi: 10.1002/ijc.31734.
6. Schlatzer, D. et al. (2017). A targeted mass spectrometry assay for detection of HIV gag protein following induction of latent viral reservoirs. Anal. Chem. 89(10):5325-5332.
7. Bianco, C. et al. (2017). Restriction of HCMV replication by ISG15, a host effector regulated by cGAS-STING dsDNA sensing. J Virol. doi: 10.1128/JVI.02483-16.
8. Bonar, M.M. et al. (2017). High sensitivity detection and sorting of infectious human immunodeficiency virus (HIV-1) particles by flow virometry. Virology. doi: 10.1016/j.virol.2017.02.016.
9. Chen, F. et al. (2017). Episomal lentiviral vectors confer erythropoietin expression in dividing cells. Plasmid. doi: 10.1016/j.plasmid.2017.02.001.
10. Kieffer, C. et al. (2017). Longitudinal imaging of HIV-1 spread in humanized mice with parallel 3D immunofluorescence and electron tomography. Elife. doi: 10.7554/eLife.23282.
11. Li, J. et al. (2016). Artemisinins target GABA A receptor signaling and impair a cell identity. Cell doi:10.1016/j.cell.2016.11.010.
12. Haqqani, A. A. et al. (2015). Central memory CD4+ T cells are preferential targets of double infection by HIV-1. Virol J.  12:184.
13. Lin, B. et al. (2015). Use of a novel integrase-deficient lentivirus for targeted anti-cancer therapy with survivin promoter-driven diphtheria toxin A. Medicine (Baltimore). 94:e1301.
14. Oh, S. M. et al. (2015). Combined Nurr1 and Foxa2 roles in the therapy of Parkinson's disease. EMBO Mol Med. doi: 10.15252/emmm.201404610. 
15. Tilton, C. A. et al. (2014). A combination HIV reporter virus system for measuring post-entry event efficiency and viral outcome in primary CD4+ T cell subsets.J Virol Methods. 195:164-169.
16. Lucera, M.B. et al. (2014).  The Histone Deacetylase Inhibitor Vorinostat (SAHA) Increases the Susceptibility of Uninfected CD4+ T Cells to HIV by Increasing the Kinetics and Efficiency of Postentry Viral Events. J Virol. 88:10803-10812.
17. Tabler, C.O. et al. (2014).  CD4+ Memory Stem Cells Are Infected by HIV-1 in a Manner Regulated in Part by SAMHD1 Expression. J Virol. 88:4976-4986.
18. Mincheva-Tasheva, S. (2014). Apoptotic cell death and altered calcium homeostasis caused by frataxin depletion in dorsal root ganglia neurons can be prevented by BH4 domain of Bcl-xL protein. Hum. Mol. Genet. 23:1829-1841.
19. Yi, S.H. et al. (2014). Foxa2 Acts as a Co-Activator Potentiating Expression of the Nurr1-Induced DA Phenotype via Epigenetic Regulation. Development. 141:761-772.
20. Tasheva, S. et al. (2013). Apoptotic cell death and altered calcium homeostasis caused by frataxin depletion in dorsal root ganglia neurons can be prevented by BH4 domain of Bcl-xL protein. Human. Mol. Genet. doi: 10.1093/hmg/ddt576.
21. Kong, L. et al. (2013). Interferon Alfa Partially Inhibits HIV Replication in Hepatocytes In Vitro. The Journal of Infectious Disease. 99:632-639.
22. Smith, B. et al. (2013). Targeting the PyMT Oncogene to Diverse Mammary Cell Populations Enhances Tumor Heterogeneity and Generates Rare Breast Cancer Subtypes. Genes & Cancer. 10.1177/1947601913475359.
23. Iftikhar, M. et al. (2011). Lysyl Oxidase-like-2 (LOXL2) is a Major Isoform in Chondrocytes and is Critically Required for Differentiation. J. Biol. Chem. 286:909-918.
24. Agrawai-Gamse, C. et al. (2010). Yeast-elicited cross-reactive antibodies to HIV Env glycans efficiently neutralize virions expressing exclusively high-mannose N-Linked glycans. J. Virol. 85(1):470-480.
25. Piedrahita, D. et al. (2010). Silencing of CDK5 Reduces Neurofibrillary Tangles in Transgenic Alzheimer's Mice. J. Neurosci. 30:13966-13976.
26. Agrawai-Gamse, C. et al. (2010). Yeast-Elicited Cross-Reactive Antibodies to HIV Env Glycans Efficiently Neutralize Virions Expressing Exclusively High-mannoseN-Linked Glycans. J. Virol 10.1128/JVI.01349-10.
27. Young, A.P. and Wagers, A.J. (2010). Pax3 Induces Differentiation of Juvenile Skeletal Muscle Stem Cells Without Transcriptional Upregulation of Canonical Myogenic Regulatory Factors. J. Cell Sci. 123: 2632-2639.
28. Brittain, J.M. et al. (2009). An Atypical Role for Collapsin Response Mediator Protein (CRMP-2) in Neurotransmitter Release via Interaction with Persynaptic Voltage-Gated Calcium Channels. J. Biol. Chem. 284:31375-31390.
29. Shu, L. et al. (2009). The mTORC2 complex regulates terminal differentiation of C2C12 myoblasts. Mol. Cell. Biol. 29:4691-4700.
30. Agrawal-Gamse, C. et al. (2009). Adaptive mutations in a human immunodeficiency virus type 1 envelope protein with a truncated V3 loop restore function by improving interactions with CD4. J. Virol. 83:11005-11015.

   Lentivirus Rapid Quantitation Kit (VPK-112)

  • purified unpurified supernatant viral nucleic acid մϴ.
  • 45-60 ̿ ż ֽϴ.
  • Limit of detection: 10^10 viral particles/mL from 100 µL of lentiviral supernatant

Recent Product Citations
1. Li, J. et al. (2020). Nna1 gene deficiency triggers Purkinje neuron death by tubulin hyperglutamylation and ER dysfunction. JCI Insight. 5(19):136078. doi: 10.1172/jci.insight.136078.
2. Huang, L. et al. (2019). Time-restricted release of multiple neurotrophic factors promotes axonal regeneration and functional recovery after peripheral nerve injury. FASEB J. fj201802065RR. doi: 10.1096/fj.201802065RR.
3. Huang, L. et al. (2018). Beta-catenin promotes macrophage-mediated acute inflammatory response after myocardial infarction. Immunol Cell Biol. 96(1):100-113. doi: 10.1111/imcb.1019.
4. Pardee, K. et al. (2016). Rapid, low-cost detection of Zika virus using programmable biomolecular components. Cell. 165:1-12.
5. Luo, H. et al. (2016). EphrinB1 and EphrinB2 regulate T cell chemotaxis and migration in experimental autoimmune encephalomyelitis and multiple sclerosis. Neurobiol Dis. 91:292-306.
6. Georgiadis, M. M. et al. (2016). Small molecule activation of apurinic/apyrimidinic endonuclease 1 reduces DNA damage induced by cisplatin in cultured sensory neurons. DNA Repair.  doi:10.1016/j.dnarep.2016.03.009.
7. Lambert, M. P. et al. (2015). Intramedullary megakaryocytes internalize released platelet factor 4 (PF4) and store it in alpha granules. J Thromb Haemost. doi:10.1111/jth.13069. 
8. Kim, H. Suk. et al. (2015). APE1, the DNA base excision repair protein, regulates the removal of platinum adducts in sensory neuronal cultures by NER.Mutat Res-Fund Mol M. doi:10.1016/j.mrfmmm.2015.06.010.
9. Yadavilli, S. et al. (2015). The emerging role of NG2 in pediatric diffuse intrinsic pontine glioma. Oncotarget.  6:12141-12155.
10. Ahmad, M. A. et al. (2015). Label-free capacitance-based identification of viruses. Sci Rep.5:9809.
11. Tang, X. et al. (2015). The advantages of PD1 activating chimeric receptor (PD1-ACR) engineered lymphocytes for PDL1+ cancer therapy. Am J Transl Res. 7:460-473.
12. Giraldo, D. M. et al. (2015).  Impact of in vitro costimulation with TLR2, TLR4 and TLR9 agonists and HIV-1 on antigen-presenting cell activation. Intervirology. 58:122-129.
13. Shin, H. S. et al. (2015). Crosstalk among IL-23 and DNAX activating protein of 12 kDa-dependent pathways promotes osteoclastogenesis.J Immunol. 194:316-324.
14. Al Ahmad, M. et al. (2014). Virus detection and quantification using electrical parameters. Sci Rep. 4:6831.
15. Fan, X. et al. (2014). Endometrial VEGF induces placental sFLT1 and leads to pregnancy complications. J Clin Invest. 124:4941-4952.
16. Zhao, S. L. et al. (2014). Mesenchymal stem cells with overexpression of midkine enhance cell survival and attenuate cardiac dysfunction in a rat model of myocardial infarction. Stem Cell Res Ther. 5:37.
17. Rossello, R.A. et al. (2013). Mammalian Genes Induce Partially Reprogrammed Pluripotent Stem Cells in Non-Mammalian Vertebrate and Invertebrate Species. eLife Sci. 2:e00036.
18. Fan, X. et al. (2012). Transient, Inducible, Placenta-Specific Gene Expression in Mice. Endocrinology. 153: 5637-5644 (#VPK-112). 
19. Veeraraghavalu, K. et al. (2010). Presenilin 1 Mutants Impair the Self-Renewal and Differentiation of Adult Murine Subventricular Zone-Neuronal Progenitors via Cell-Autonomous Mechanisms Involving Notch Signaling. J. Neurosci. 30:6903-6915.
20. Niwano, K. et al. (2008). Lentiviral vector-mediated SERCA2 gene transfer protects against heart failure and left ventricular remodeling after myocardial infarction in rats. Mol. Ther. 16:1026-1032.