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 제품안내  항체,항원,킷트   Cell Analysis  Cell Migration/Invasion

Cell Migration & Invasion Assay Kit (Boyden chamber & Gap closure)

Cell migration과 invasion assay에 이용되는 제품을 소개합니다.

Cell Biolabs
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 ▶ Boyden Chamber Assays
  • Boyden chamber를 이용하여 migration, invasion을 연구하는 방법입니다.
  • pore size를 가진 membrane으로 구성된 원통형 insert를 cell culture plate의 well에 넣고 cell을 seeding 후 chemoattractant를 함께 배양하여 cell의 이동성을 확인합니다.
  • insert에 ECM을 코팅하여 cell의 invasion을 확인할 수 있습니다.
  • Chemotaxis, Haptotaxis, leukocyte나 tumor cell의 Transmigration 연구에 유용합니다. 
 Assay Type
 Cell Types  Pore
 Size
 Insert Coating  Formats  Cat. No
 Chemotaxis Leukocytes, Neutrophils 3 µm None 24-Well
96-Well
CBA-103
CBA-104
Lymphocytes
Macrophages
Monocytes
5 µm None 24-Well
96-Well
CBA-102
CBA-105
Endothelial Cells
Epithelial Cells
Fibroblasts
8 µm None 24-Well
96-Well
CBA-100
CBA-106
Astrocytes Slow-moving Cells 12 µm None 24-Well CBA-107 
 Haptotaxis Endothelial Cells
Epithelial Cells
Fibroblasts
8 µm Collagen I (bottom)
Fibronectin (bottom)
24-Well
24-Well
CBA-100-COL
CBA-100-FN
 Transmigration Leukocytes
Tumor Cells
3 µm
8 µm
None
None
24-Well
24-Well
CBA-212
CBA-216
 Invasion Endothelial Cells
Epithelial Cells
Fibroblasts
Tumor Cells
8 µm ECM Matrix (top) 24-Well
96-Well
CBA-110
CBA-112
Collagen I  24-Well
96-Well
CBA-111-COL
CBA-112-COL
Laminin I (top) 24-Well
96-Well
CBA-110-LN
CBA-112-LN

 

 ▶ Gap Closure Assays
  • Boyden Chamber 방법을 변형시킨 형태로, Hydrogel (Radius gel) spot을 이용하여 세포의 Migration을 관찰합니다.
  • 정성, 정량, 종말점 및 실시간 분석이 가능하며, Time course migration study에 적합합니다.
  • Cell culture insert를 사용하지 않으므로, Pore size와 상관없이 여러 타입의 세포를 사용할 수 있습니다.
  • Ucoated, Collagen Ⅰ-coated, Fibronectin-coated, Laminin-coated 혹은 ECM Array(Ucoated, Collagen Ⅰ, Fibronectin, Laminin) 등 5가지 종류의 Kit으로 제공됩니다.




 

 2D Gap Closure AssaysBoyden Chamber Assays
Type of Analysis Qualitative or Quantitative Quantitative
Detection Time Endpoint or Real Time Endpoint
Detection Method Microscopy Plate Reader
Chemoattractant Gradient NO Yes
Relative Sensitivity Good Fair
Adaptability to Automation Good Poor
Cell Compatibility Universal Choose membrane pore size based
on cell type/size


 
주문정보(Ordering Information)
CATNUM PRODUCT SIZE
CBA-100-COLCytoSelect™ 24-well Cell12 assays
CBA-101-COLCytoSelect™ 24-well Cell12 assays
CBA-100-FNCytoSelect™ 24-well Cell12 assays
CBA-101-FNCytoSelect™ 24-well Cell12 assays
CBA-110CytoSelect™ 24-well Cell Invasion Assay, Colorimetric12 assays
CBA-111CytoSelect™ 24-well Cell Invasion, Fluorometric12 assays
CBA-100-CCytoSelect™ 24-well Cell Migration and Invasion Assay (8 2 x 12 assays
CBA-100-C-5CytoSelect™ 24-well Cell Migration and Invasion Assay (8 10 x 12 assays
CBA-101-CCytoSelect™ 24-well Cell Migration and Invasion Assay (8 2 x 12 assays
CBA-107CytoSelect™ 24-well Cell Migration Assay (12 μm), Colorimetric12 assays
CBA-108CytoSelect™ 24-well Cell Migration Assay (12 μm), Fluorometric12 assays
CBA-103-TCytoSelect™ 24-Well Cell Migration Assay (3 4 assays
CBA-103CytoSelect™ 24-well Cell Migration Assay (3 μm), Fluorometric12 assays
CBA-103-5CytoSelect™ 24-well Cell Migration Assay (3 μm), Fluorometric5 x 12 assays
CBA-102-TCytoSelect™ 24-Well Cell Migration Assay (5 4 assays
CBA-102CytoSelect™ 24-well Cell Migration Assay (5 μm), Fluorometric12 assays
CBA-102-5CytoSelect™ 24-well Cell Migration Assay (5 μm), Fluorometric5 x 12 assays
CBA-100-TCytoSelect™ 24-Well Cell Migration Assay (8 4 assays
CBA-101-TCytoSelect™ 24-Well Cell Migration Assay (8 4 assays
CBA-100-5CytoSelect™ 24-well Cell Migration Assay (8 μm), Colorimetric5 x 12 assays
CBA-100CytoSelect™ 24-well Cell Migration Assay (8 μm), Colorimetric12 assays
CBA-101CytoSelect™ 24-well Cell Migration Assay (8 μm), Fluorometric12 assays
CBA-101-5CytoSelect™ 24-well Cell Migration Assay (8 μm), Fluorometric5 x 12 assays
CBA-111-COLCytoSelect™ 24-well Collagen Cell Invasion Assay, Fluorometric12 assays
CBA-110-COLCytoSelect™ 24-well Collagen Cell Invasion, Colorimetric12 assays
CBA-111-LNCytoSelect™ 24-well Laminin Cell Invasion Assay, Fluorometric12 assays
CBA-110-LNCytoSelect™ 24-well Laminin Cell Invasion, Colorimetric12 assays
CBA-112CytoSelect™ 96-well Cell Invasion, Fluorometric96 assays
CBA-106-CCytoSelect™ 96-well Cell Migration and Invasion Assay (8 2 x 96 assays
CBA-104-5CytoSelect™ 96-well Cell Migration Assay (3 μm), Fluorometric5 x 96 assays
CBA-104CytoSelect™ 96-well Cell Migration Assay (3 μm), Fluorometric96 assays
CBA-105CytoSelect™ 96-well Cell Migration Assay (5 μm), Fluorometric96 assays
CBA-105-5CytoSelect™ 96-well Cell Migration Assay (5 μm), Fluorometric5 x 96 assays
CBA-106-5CytoSelect™ 96-well Cell Migration Assay (8 μm), Fluorometric5 x 96 assays
CBA-106CytoSelect™ 96-well Cell Migration Assay (8 μm), Fluorometric96 assays
CBA-112-COLCytoSelect™ 96-well Collagen Cell Invasion Assay, Fluorometric96 assays
CBA-112-LNCytoSelect™ 96-well Laminin Cell Invasion Assay, Fluorometric96 assays
CBA-212CytoSelect™ Leukocyte Transmigration Assay24 assays
CBA-125Radius™ 24-Well Cell Migration Assay24 assays
CBA-125-5Radius™ 24-Well Cell Migration Assay5 x 24 assays
CBA-125-COLRadius™ 24-Well Cell Migration Assay, (Collagen I Coated)24 assays
CBA-125-ECMRadius™ 24-Well Cell Migration Assay, (ECM Array Coated)24 wells
CBA-125-FNRadius™ 24-Well Cell Migration Assay, (Fibronectin Coated)24 assays
CBA-125-LNRadius™ 24-Well Cell Migration Assay, (Laminin Coated)24 assays
CBA-127-5Radius™ 384-Well Cell Migration Assay5 x 384 wells
CBA-127Radius™ 384-Well Cell Migration Assay384 assays
CBA-5037-5Radius™ 48-Well Cell Migration Assay5 x 48 assays
CBA-5037Radius™ 48-Well Cell Migration Assay48 assays
CBA-126-5Radius™ 96-Well Cell Migration Assay5 x 96 assays
CBA-126Radius™ 96-Well Cell Migration Assay 96 assays