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 제품안내  면역학   Cell Analysis

Cell Viability and Cytotoxicity Assay Kit

세포의 생존과 사멸을 확인하기 위해 사용되는 제품들입니다.

Cell Biolabs, Biotium
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Kit 종류 원리 Cat. No
ATP-Glo™ Bioluminometric Cell Viability Assay live cell의 ATP가 D-Luciferin을 산화하면서 발생하는 빛을 측정 30020-1
30020-2
Calcein AM Cell Viability Assay Kit live cell에서 calcein AM이 가수분해 되어 green fluorescent calcein으로 전환됨 30026
CytoSelect™ Cell Viability and Cytotoxicity Assay 세포의 metabolic activity 이용. live cell은 MTT 혹은 Calcein AM을 이용하며, Dead cell은 EthD-1을 이용하여 확인 CBA-240
CytoSelect™ Anoikis Assay Kit Adherent cell이 Extracellular matrix (ECM)에 부착되지 못하거나 잘못 부착되어 apoptosis가 일어나는 경우, Anoikis(Anchorage-Dependent Cell Death)를 확인 CBA-080
CBA-081
MTT Cell Viability Assay Kit live cell에서만 존재하는 mitochondrial enzyme에 의해 reduction 되면 purple formazan을 형성하는 MTT tetrazolium salt을 측정 30006
XTT Cell Viability Assay Kit MTT assay와 원리는 동일하나 XTT는 수용성 화합물로 환원되어 별도의 solubilization 단계 없이 측정 가능 30007
Resazurin Cell Viability Assay Kit live cell 존재 시, redox indicator인 resazurin이 nonfluorescne dye에서 red fluorescent dye인 resorufin으로 환원 30025-1
Live-or-Dye™ Fixable Viability Staining Kits  유세포 분석 (flow cytometry)중 live, dead cells을 구분하는 제품. 원하는 형광파장을 선택할 수 있음 32002 - 32010
Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells 진핵세포의 live cell은 calcein AM에 의해 녹색으로, dead cell은 Ethidium homodimer III (EthD-III)에 의해 붉은색으로 형광염색됨 30002






ATP-Glo™ Bioluminometric Cell Viability Assay Kit
Luminescence dose response curves. A. Ten-fold dilutions of ATP were prepared in 100 uL PBS per sample. B.Ten-fold dilutions of Jurkat cells were prepared in 100 uL RPMI medium per sample. Immediately before luminescence measurement, 100 uL of ATP-Glo detection cocktail was added to each sample. Luminescence was measured for 10 seconds using a Turner Designs single tube luminometer.


 
CytoSelect™ Cell Viability and Cytotoxicity Assay 
Human Foreskin Fibroblast BJ-TERT Cell Viability. BJ-TERT cells were seeded at 50,000 cells/well and allowed to culture for 24 hours. Cells were then treated with and without Saponin. Cell samples were then treated with MTT, Calcein AM or EthD-1.


CytoSelect™ 24-Well Anoikis Assay 
Anoikis Assay of Human Foreskin Fibroblast BJ-TERT Cells. BJ-TERT cells were seeded at 50,000 cells/well in a tissure culture control plate or a Poly-Hema coated plate. Cells were allowed to culture for 24 hours. Cell viability was determined by MTT and Calcein AM, while anoikis-like cell death was stained with EthD-1.