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 제품안내  면역학   Cell Structure  Tubulin Assay

Kinesin Assay Kit

ATP를 가수분해하며 발생하는 에너지를 이용하여 Microtubule 위를 이동하는 Kinesin 연구를 위한 제품입니다.

Cytoskeleton
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종류와 특징
  • HTS kinesin ATPase Endpoint Assay Biochem Kit
    • Kinesin motor protein의 활성에 의해 가수분해가 진행되는 동안 생성되는 Inorganic Phosphate (Pi)를 빠르고 쉽게 측정하는 제품입니다.
    • Colorimetric 방법을 이용하여 측정하며, HTS screen에 유용합니다.
    • Kit 구성품
      • CytoPhos™ reagent
      • Kinesin motor protein
      • Pre-formed microtubules
      • Kinesin Reaction Buffer
      • Paclitaxel
      • Phosphate standard
      • DMSO
  • Kinesin ELIPA kit
    • 미세소관에서 활성화된 Kinesin의 ATPase 활성을 측정하여 Kinesin을 정량합니다.
    • Kinesin Inhibitor의 IC50 측정이 가능하여, Kinesin Inhibitor 연구에 유용합니다.
    • Kit 구성품
      • Reaction buffer
      • ELIPA reagent 1 (MESG)
      • ELIPA reagent 2 (PNP)
      • Kinesin Heavy Chain motor domain protein
      • Pre-formed microtubules
      • Paclitaxel
      • Phosphate standard
      • ELIPA reagent 1 resuspension buffer
      • ATP stock
      • GTP stock
  • Kinesin Motility Assay Biochem Kit
    • 단백질이나 화합물 등의 샘플이 Kinesin 혹은 Dynein-microtubule 이동성을 변경시키는지 측정하는 제품입니다.
    • Kinesin 혹은 Dynein motor는 Microscope perfusion chamber의 표면에 커플링되어 제공됩니다.
    • 미세소관의 Motor-induced motility는 형광현미경을 이용하여 모니터링하고 측정할 수 있습니다.
    • Kit 구성품
      • Tubulin, >99% pure, lyophilized
      • Rhodamine tubulin, lyophilized
      • Kinesin heavy chain motor domain, lyophilized (positive control)
      • Antifade
      • ATP
      • Microtubule polymerization buffer
      • Tubulin glycerol buffer
      • Microtubule resuspension buffer
      • Blocking solution
      • Chamber wash buffer
      • Motility buffer
      • Paclitaxel
      • DMSO
      • 25 acid washed perfusion chambers
      • Chamber filters
적용
Figure 1. Inhibition of kinesin activity with the Eg5 specific inhibitor monastrol.
Figure 1. Inhibition of kinesin activity with the Eg5 specific inhibitor monastrol. The results show percent of full kinesin activity in the presence of 100 uM monastrol. As expected, only Eg5 ATPase activity is inhibited by monastrol.
Figure 2. Microtubule activated kinesin ATPase activity as measured with BK060.
Figure 2. Microtubule activated kinesin ATPase activity as measured with BK060. The ATPase activities of a selection of kinesin motors was measured. The Vmax for ATP hydrolysis ranges from 10,000 nmol ATP/min/mg for Kinesin HC to 470 nmol ATP/min/mg for Chromokinesin.
Figure 3. Microtubule Motility Assay.
Figure 3. Microtubule Motility Assay. The field was selected to show a sparse area of MT coverage in order to allow clear visualization of MT motility. Each frame represents a 5 min time-point, from 0 to 15 min. The position of each MT at time zero is marked by an arrow. The arrow position remains identical in each frame to serve as a reference point for MT movement. To obtain an average MT velocity, we recommend measure motility of approximately 30 - 50 individual MTs.
주문정보(Ordering Information)
CATNUM PRODUCT SIZE
BK060Kinesin ELIPA™ Biochem Kit1 Kit (96 Assays)
BK053Kinesin HTS ATPase end point Assay1000 assays
BK027Kinesin/dynein motility Kit25 assays