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¼¼Æ÷ ³» Small GTP-binding proteins(GTPases)ÀÇ È°¼ºÀ» À¯»öÀÇ Agarose bead¸¦ ÀÌ¿ëÇÏ¿© ¼±º°ÀûÀ¸·Î ħÀü(Pull-down¹æ½Ä)½ÃÄÑ ÃøÁ¤ÇÏ´Â Á¦Ç°ÀÔ´Ï´Ù. |
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Cell Biolabs |
** Small GTPase recombinant proteins
STA-728 | Recombinant Human Rac1, His Tagged | 50 µg |
STA-732 | Recombinant Human Ral A | 25 µg |
STA-733 | Recombinant Human Ral B | 10 µg |
STA-735 | Recombinant Human Rap1a | 10 µg |
STA-740 | Recombinant Human RhoA | 20 µg |
STA-747 | Recombinant Human H-Ras | 25 µg |
STA-748 | Recombinant Human K-Ras | 25 µg |
STA-749 | Recombinant Human N-Ras, Untagged | 10 µg |
Figure 1: Immunoblotting with the Rac Activation Assay. Lane 1: GTPase Immunoblot Positive Control. Lane 2: 293 cell lysate loaded with GDP and incubated with PAK1 PBD Agarose beads. Lane 3: 293 cell lysate loaded with GTPγS and incubated with PAK1 PBD Agarose beads.
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