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 Á¦Ç°¾È³»  Ç×ü,Ç׿ø,ŶƮ   Cell Signaling  G-Protein Signaling

Small GTPase Activation Assay Kit

¼¼Æ÷ ³» Small GTP-binding proteins(GTPases)ÀÇ È°¼ºÀ» À¯»öÀÇ Agarose bead¸¦ ÀÌ¿ëÇÏ¿© ¼±º°ÀûÀ¸·Î ħÀü(Pull-down¹æ½Ä)½ÃÄÑ ÃøÁ¤ÇÏ´Â Á¦Ç°ÀÔ´Ï´Ù.

Cell Biolabs
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  • À¯»öÀÇ Agarose bead¸¦ ÀÌ¿ëÇÏ¿© À°¾ÈÀ¸·Îµµ ½±°Ô È®ÀÎÀÌ °¡´ÉÇÕ´Ï´Ù.
  • Non-radioactive ¹æ½ÄÀ¸·Î ¾ÈÀüÇÏ°Ô »ç¿ëÇÒ ¼ö ÀÖ½À´Ï´Ù.
  • Target-specific antibody°¡ ÇÔ²² Á¦°øµË´Ï´Ù.
  • 1½Ã°£ ¾È¿¡ °á°ú¸¦ ¾òÀ» ¼ö ÀÖÀ¸¸ç, Immunoblot ȤÀº ECL solutionÀ» ÀÌ¿ëÇÏ¿© DetectionÇÕ´Ï´Ù.
  • ºÐ¼® °¡´ÉÇÑ Å¸°Ù :
    • Arf1 and Arf6
    • Cdc42
    • Rac1 and Rac2
    • Ral
    • Ran
    • Rap1 and Rap2
    • H-Ras, K-Ras, N-Ras, and Pan-Ras
    • RhoA, RhoB, and RhoC

 

   ** Small GTPase recombinant proteins

STA-728  Recombinant Human Rac1, His Tagged 50 µg
STA-732  Recombinant Human Ral A 25 µg
STA-733  Recombinant Human Ral B 10 µg
STA-735  Recombinant Human Rap1a 10 µg
STA-740  Recombinant Human RhoA 20 µg
STA-747  Recombinant Human H-Ras 25 µg
STA-748  Recombinant Human K-Ras 25 µg
STA-749  Recombinant Human N-Ras, Untagged 10 µg


Small GTPase Activation Assay ¿ø¸®

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Figure 1: Immunoblotting with the Rac Activation Assay

Figure 1: Immunoblotting with the Rac Activation Assay. Lane 1: GTPase Immunoblot Positive Control. Lane 2: 293 cell lysate loaded with GDP and incubated with PAK1 PBD Agarose beads. Lane 3: 293 cell lysate loaded with GTPγS and incubated with PAK1 PBD Agarose beads.

ÁÖ¹®Á¤º¸(Ordering Information)
CATNUM PRODUCT SIZE
STA-44196-Well Ras Activation ELISA Kit (Chemiluminescent)96 assays
STA-44096-Well Ras Activation ELISA Kit (Colorimetric)96 assays
STA-459Active H-Ras Expression Vector Set1 kit
STA-422Active Rac-GEF Assay Kit (Tiam1)20 assays
STA-458Active Rac1 Expression Vector Set1 kit
STA-407-1Arf1 Activation Assay20 assays
STA-407-6Arf6 Activation Assay20 assays
STA-402Cdc42 Activation Assay20 assays
STA-455Cdc42 Expression Vector Set1 kit
STA-433Cdc42 G15A Agarose Beads (Active Cdc42-GEF)800 ug
STA-400-HH-Ras Activation Assay20 assays
STA-457H-Ras Expression Vector Set1 kit
STA-400-KK-Ras Activation Assay20 assays
STA-400-NN-Ras Activation Assay20 assays
STA-400Pan-Ras Activation Assay20 assays
STA-401-1Rac1 Activation Assay20 assays
STA-454Rac1 Expression Vector Set1 kit
STA-432Rac1 G15A Agarose Beads (Active Rac-GEF)800 ug
STA-404Rac1/Cdc42 Activation Assay Combo Kit2 x 20 assays
STA-401-2Rac2 Activation Assay20 assays
STA-410Raf-1 RBD Agarose Beads400 ug
STA-408Ral Activation Assay20 assays
STA-420RalBP1 PBD Agarose Beads400 ug
STA-418RalGDS RBD Agarose Beads400 ug
STA-409Ran Activation Assay20 assays
STA-421RanBP1 Agarose Beads400 ug
STA-406-1Rap1 Activation Assay Kit20 assays
STA-406-2Rap2 Activation Assay Kit20 assays
STA-747Recombinant Human H-Ras25 ug
STA-748Recombinant Human K-Ras25 ug
STA-749Recombinant Human N-Ras, Untagged10 ug
STA-728Recombinant Human Rac1, His Tagged50 ug
STA-732Recombinant Human Ral A25 ug
STA-735Recombinant Human Rap1a10 ug
STA-740Recombinant Human RhoA20 ug
STA-403-ARhoA Activation Assay20 assays
STA-456RhoA Expression Vector Set1 kit
STA-431RhoA G17A Agarose Beads (Active Rho-GEF)400 ug
STA-405RhoA/Rac1/CDC42 Activation Assay Combo Kit3 x 10 assays
STA-403-BRhoB Activation Assay20 assays
STA-403-CRhoC Activation Assay20 assays