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 제품안내  항체,항원,킷트   Cell Signaling  G-Protein Signaling

Small GTPase Activation Assay Kit

세포 내 Small GTP-binding proteins(GTPases)의 활성을 유색의 Agarose bead를 이용하여 선별적으로 침전(Pull-down방식)시켜 측정하는 제품입니다.
Cell Biolabs
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특징
  • 유색의 Agarose bead를 이용하여 육안으로도 쉽게 확인이 가능합니다.
  • Non-radioactive 방식으로 안전하게 사용할 수 있습니다.
  • Target-specific antibody가 함께 제공됩니다.
  • 1시간 안에 결과를 얻을 수 있으며, Immunoblot 혹은 ECL solution을 이용하여 Detection합니다.
  • 분석 가능한 타겟 :
    • Arf1 and Arf6
    • Cdc42
    • Rac1 and Rac2
    • Ral
    • Ran
    • Rap1 and Rap2
    • H-Ras, K-Ras, N-Ras, and Pan-Ras
    • RhoA, RhoB, and RhoC

 

   ** Small GTPase recombinant proteins

STA-728  Recombinant Human Rac1, His Tagged 50 µg
STA-732  Recombinant Human Ral A 25 µg
STA-733  Recombinant Human Ral B 10 µg
STA-735  Recombinant Human Rap1a 10 µg
STA-740  Recombinant Human RhoA 20 µg
STA-747  Recombinant Human H-Ras 25 µg
STA-748  Recombinant Human K-Ras 25 µg
STA-749  Recombinant Human N-Ras, Untagged 10 µg


Small GTPase Activation Assay 원리

적용

Figure 1: Immunoblotting with the Rac Activation Assay

Figure 1: Immunoblotting with the Rac Activation Assay. Lane 1: GTPase Immunoblot Positive Control. Lane 2: 293 cell lysate loaded with GDP and incubated with PAK1 PBD Agarose beads. Lane 3: 293 cell lysate loaded with GTPγS and incubated with PAK1 PBD Agarose beads.

주문정보(Ordering Information)
CATNUM PRODUCT SIZE
STA-44196-Well Ras Activation ELISA Kit (Chemiluminescent)96 assays
STA-44096-Well Ras Activation ELISA Kit (Colorimetric)96 assays
STA-459Active H-Ras Expression Vector Set1 kit
STA-422Active Rac-GEF Assay Kit (Tiam1)20 assays
STA-458Active Rac1 Expression Vector Set1 kit
STA-407-1Arf1 Activation Assay20 assays
STA-407-6Arf6 Activation Assay20 assays
STA-402Cdc42 Activation Assay20 assays
STA-455Cdc42 Expression Vector Set1 kit
STA-433Cdc42 G15A Agarose Beads (Active Cdc42-GEF)800 ug
STA-400-HH-Ras Activation Assay20 assays
STA-457H-Ras Expression Vector Set1 kit
STA-400-KK-Ras Activation Assay20 assays
STA-400-NN-Ras Activation Assay20 assays
STA-400Pan-Ras Activation Assay20 assays
RTV-202pBABEhygro-Rac1 V12 Retroviral Vector100 uL
RTV-204pBABEhygro-RhoA L63 Retroviral Vector100 uL
RTV-220pBABEpuro-K-Ras Retroviral Vector100 uL
RTV-201pBABEpuro-myr-Rac1 Retroviral Vector100 uL
RTV-101pBABEpuro-Ras V12 Retroviral Vector100 uL
STA-401-1Rac1 Activation Assay20 assays
STA-454Rac1 Expression Vector Set1 kit
STA-432Rac1 G15A Agarose Beads (Active Rac-GEF)800 ug
STA-404Rac1/Cdc42 Activation Assay Combo Kit2 x 20 assays
STA-401-2Rac2 Activation Assay20 assays
STA-410Raf-1 RBD Agarose Beads400 ug
STA-408Ral Activation Assay20 assays
STA-420RalBP1 PBD Agarose Beads400 ug
STA-418RalGDS RBD Agarose Beads400 ug
STA-409Ran Activation Assay20 assays
STA-421RanBP1 Agarose Beads400 ug
STA-406-1Rap1 Activation Assay Kit20 assays
STA-406-2Rap2 Activation Assay Kit20 assays
STA-747Recombinant Human H-Ras25 ug
STA-748Recombinant Human K-Ras25 ug
STA-749Recombinant Human N-Ras, Untagged10 ug
STA-728Recombinant Human Rac1, His Tagged50 ug
STA-732Recombinant Human Ral A25 ug
STA-735Recombinant Human Rap1a10 ug
STA-740Recombinant Human RhoA20 ug
STA-403-ARhoA Activation Assay20 assays
STA-456RhoA Expression Vector Set1 kit
STA-431RhoA G17A Agarose Beads (Active Rho-GEF)400 ug
STA-405RhoA/Rac1/CDC42 Activation Assay Combo Kit3 x 10 assays
STA-403-BRhoB Activation Assay20 assays
STA-403-CRhoC Activation Assay20 assays