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 Á¦Ç°¾È³»  ºÐÀÚ»ý¹°ÇР  Protein Research  Protein Assay Reagents

GST Protein Inclusion Body Solubilization

¹ÚÅ׸®¾Æ¿¡¼­ GST À¶ÇÕ ´Ü¹éÁúÀÌ inclusion body¸¦ Çü¼ºÇÏ¿© ¹ßÇöµÉ ¶§´Â °¡¿ëÈ­ µÇ±â ¸Å¿ì ¾î·Á¿ö ģȭ¼º Å©·Î¸¶Åä±×·¡ÇÇÀÇ »ç¿ëÀÌ Á¦ÇѵǴ ¹®Á¦Á¡ÀÌ ¹ß»ýÇÕ´Ï´Ù.

Rapid GST Inclusion Body Solubilization and Renaturation KitÀº ¿ëÇØ¿Í ÃßÃâ °úÁ¤À» ÅëÇØ ¹ßÇöµÈ GST À¶ÇմܹéÁúÀ» solubleÇÑ ÇüÅ·Πº¹¿ø½ÃŰ´Â Á¦Ç° ÀÔ´Ï´Ù.

Cell Biolabs
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  • ¹ÚÅ׸®¾Æ ¹è¾ç¾× 5-10 ¸®Å͸¦ °¡¿ëÈ­ ¹× º¹¿ø½Ãų ¼ö ÀÖ´Â ÃæºÐÇÑ ¾çÀÇ ½Ã¾àµéÀÌ Æ÷ÇԵǾî ÀÖ½À´Ï´Ù.
    Kit ±¸¼ºÇ°
  • 10X STE Buffer
  • Detergent Solubilization Solution
  • Detergent Neutralization Solution
    GST Inclusion Bodies Solubilization and Renaturation Flow Chart
    GST Inclusion Bodies Solubilization and Renaturation Flow Chart
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    Figure 1: Solubilization and Renaturation of GST-RTK fusion protein.
    Figure 1: Solubilization and Renaturation of GST-RTK fusion protein.

    GST-RTK expression was induced with 1 mM IPTG at 37¡ÆC for 4 hrs. Cell pellet was lysed, and inclusion body was solubilized and renatured under different amounts of detergent solubilization solution according to the assay protocol.
    Lane 1: MW STD; Lane 2: Whole E.Coli lysate; Lane: 3, 7, 11: No detergent; Lane 4, 8, 12: 32-fold dilution; Lane 5, 9, 13: 8-fold dilution; Lane 6, 10, 14: 2-fold dilution.