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 제품안내  분자생물학   Protein Research  Protein concentration & Extraction

Bacterial Protein Extraction Reagents

기계적인 세포파괴 방법을 사용하지 않고 E.coli로 부터 재조합 단백질을 쉽고 빠르게 추출하는 시약입니다.
Cell Biolabs
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    특징
  • Sonication 등의 기계적인 방법을 사용하지 않고 E.coli로 부터 재조합 단백질을 쉽고 빠르게 추출할 수 있습니다.
  • 단일 step으로 높은 회수율을 가지며, 총 30분 이내에 추출이 가능합니다.
  • 소규모 혹은 대량의 단백질 추출이 가능합니다.
    Bacterial Protein Extraction Flow Chart
    Bacterial Protein Extraction Flow Chart
    사용 예
    Figure 1: Extraction of His-RFP fusion protein.
    Figure 1: Extraction of His-RFP fusion protein.

    His-RFP expression in BL21 was induced with 1 mM IPTG at 37ºC for 4 hrs. Cells were pelleted (left), then resuspended in Bacterial Protein Extraction Reagent for 15 minutes, according to the assay protocol. The supernatant (right) containing the extracted His-RFP was collected from the insoluble post-extraction pellet (center).
    Figure 2: Comparison of Sonication with Bacterial Protein Extraction Reagent.
    Figure 2: Comparison of Sonication with Bacterial Protein Extraction Reagent.

    Pelleted His-RFP cells were sonicated in PBS/Triton X-100 or resuspended in Bacterial Protein Extraction Reagent for 15 minutes. Lysates were then collected and tested for RFP fluorescence on a 96-well fluorometer.