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 Á¦Ç°¾È³»  ºÐÀÚ»ý¹°ÇР  Protein Research  Protein concentration & Extraction

Bacterial Protein Extraction Reagents

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Cell Biolabs
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    Bacterial Protein Extraction Flow Chart
    Bacterial Protein Extraction Flow Chart
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    Figure 1: Extraction of His-RFP fusion protein.
    Figure 1: Extraction of His-RFP fusion protein.

    His-RFP expression in BL21 was induced with 1 mM IPTG at 37¨¬C for 4 hrs. Cells were pelleted (left), then resuspended in Bacterial Protein Extraction Reagent for 15 minutes, according to the assay protocol. The supernatant (right) containing the extracted His-RFP was collected from the insoluble post-extraction pellet (center).
    Figure 2: Comparison of Sonication with Bacterial Protein Extraction Reagent.
    Figure 2: Comparison of Sonication with Bacterial Protein Extraction Reagent.

    Pelleted His-RFP cells were sonicated in PBS/Triton X-100 or resuspended in Bacterial Protein Extraction Reagent for 15 minutes. Lysates were then collected and tested for RFP fluorescence on a 96-well fluorometer.