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 제품안내  형광시약   Protein Labeling

Fluorescent ECM Proteins

Extracellular Matrix(ECM) 단백질은 세포의 부착과 분화, 증식, 이동, 전이와 생존등 세포의 기능을 조절하며, Cellular responses연구를 위한 tool로 이용됩니다.

ECM은 collagen, non-collagenouse glycoprotein, proteoglycan으로 구성되어 있으며, 이들은 세포로부터 분비되어 ECM 망을 형성합니다. Biotin, Green, Red 등의 형광으로 표지된 ECM을 만나보세요.

Cytoskeleton
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    종류
    종류 Source Conjugation Purity Cat. No.
    Laminin EHS tumor tissue rhodamine >90% pure LMN01
    HiLyte488 >90% pure LMN02
    Biotin >90% pure LMN03
    Fibronectin bovine plasma rhodamine >80% pure FNR01
    HiLyte488 >80% pure FNR02
    Biotin >80% pure FNR03
    적용
    Figure 1: Fluorescent ECM
    Lanes:
    1. Rhodamine laminin
    2. HiLyte488™ laminin
    3. Rhodamine fibronectin
    4. HiLyte488™ fibronectin
    Figure 2: Rhodamine Laminin Purity Determination
    Legend: 20 ug of unlabeled laminin (Lane 1) and 20 ug of rhodamine laminin (Lane 2) was separated by electrophoresis in a 4-20% SDS-PAGE system. The unlabeled pro-tein was stained with Coomassie Blue and visualized in white light. The rhodamine labeled protein was visual-ized under UV light. The alpha sub-unit runs at 400 kDa (top band) while the beta and gamma subunits run as a 225 kDa doublet (lower band). Protein quantitation was determined with the Precision Red™ Protein Assay Reagent (Cat. # ADV02).
    Figure 3: Absorption scan of rhodamine laminin in solution
    Legend: LMN01 was diluted with Milli-Q water and its absorbance spectrum was scanned between 250 and 750 nm. In this example, rhodamine labeling stoichiometry was calculated to be 2.7 dyes per laminin protein using the absorbancy maximum for rhodamine at 565 nm and the Beer-Lambert law. Dye extinction coefficient when protein bound is 70,000M-1cm-1.
    Figure 4: HiLyte Fluor™ 488 labeled Fibronectin Purity Determination
    Legend: 20 ug of unlabeled fibronection (Lane 1) and 20 ug of HiLyte Fluor™ 488 labeled fibronectin (Lane 2) was separated by electrophoresis in a 4-20% SDS-PAGE system. The unlabeled protein was stained with Coomassie Blue and visualized in white light. The HiLyte Fluor™ 488 labeled protein was visualized under UV light, no free dye was observed in the dye front.
    Protein quantitation was determined with the Precision Red™ Protein Assay Reagent(Cat. #ADV02).
    Figure 5: Absorption scan of HiLyte Fluor™ 488 labeled fibronectin in solution
    Legend: FNR02 was diluted with MILLI-Q water and its absorbance spectrum was scanned between 250 and 650 nm. HiLyte Fluor™ 488 labeling stoichiometry was calculated to be 1-3 dyes per fibronectin protein using the absorbancy maximum for at 527 nm and the Beer-Lambert law. Dye extinction coefficient when protein bound is 70,000M-1cm-1