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 제품안내  형광시약   Protein Labeling  CF™ Dyes

CF™750, CF™770 and CF™790 Dyes

현존하는 dye에 비해 훨씬 밝은빛을 내는 형광 dye로, in vivo에 사용시 덜 immunogenic하게 인식되어 in vivo imaging에도 사용할수 있습니다.

Biotium
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    특징
  • 작은 동물을 이용한 in vio Imaging 장비에 사용할 수 있습니다.
  • WB시 cross-reactivity와 백그라운드를 최소화 해 줍니다.
  • 형광이 밝고, 오래 유지됩니다.
    사양
    CF™750
    • Abs/Em Maxima: 755/777 nm
    • Extinction coefficient: 250,000
    • Molecular weight: ~ 3009
    • Excitation laser line: 633 nm, 635 nm, 680 nm or 685 nm
    • Direct replacement for: Alexa Fluor® 750, Cy™7, DyLight™ 750, APC- Alexa Fluor® 750
    CF™770
    • Abs/Em Maxima: 770/797 nm
    • Extinction coefficient: 220,000
    • Molecular weight: ~ 3138
    • Excitation laser line: 785 nm
    • Direct replacement for: DyLight™ 800, IRDye 800CW
    CF™790
    • Abs/Em Maxima: 784/806 nm
    • Extinction coefficient: 210,000
    • Molecular weight: ~ 3267
    • Excitation laser line: 785 nm
    • Direct replacement for: Alexa Fluor® 790
    적용
    Figure 1. Stability of Cy7, AlexaFluor750 and CF750 dyes. Shown are the absorption spectra of the respective dyes before (black line) and after (gray line) 30 minutes of exposure to sunlight.
    Figure 2. Tumors in mice were imaged 24 and 96 hrs after i.v. injection of Avastin conjugated CF750 using an IVIS imaging system (Caliper LS).
    Images courtesy of Caliper Life Sciences.
    Figure 3. Goat anti-mouse IgG was labeled with CF750, AlexaFluor750 or Cy7, respectively. Fluorescence values were measured on a Hitachi F-4500 fluorometer. The normalized fluorescence values are shown above.
    Figure 4. Two-fold dilutions of HeLa cell lysate were run on an acrylamde gel, transferred to a nitrocellulose membrane and probed with mouse alpha-tubulin and rabbit COX IV primary antibodies followed by goat anti-mouse CF770 or IRDye 800 (green) and goat anti-rabbit CF680 or IRDye680 (red) at the same final concentrations. After probing, membranes were dried and scanned using an Odyssey infrared imaging system (Li-Cor Biosciences). Quantitation of the bands showed approximately a 3.5-fold higher fluorescence intensity of CF dyes compared to the respective IRDye secondary antibodies (Li-Cor).
주문정보(Ordering Information)
CATNUM PRODUCT SIZE
29006Annexin V, CF750 conjugate, lyophilized25 ug
20298-1CF750 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL50 uL
20298CF750 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL0.25 mL
20070-1CF750 Goat Anti-Mouse IgG (H+L), 2 mg/mL50 uL
20070CF750 Goat Anti-Mouse IgG (H+L), 2 mg/mL0.5 mL
20073-1CF750 Goat Anti-Rabbit IgG (H+L), 2 mg/mL50 uL
20073CF750 Goat Anti-Rabbit IgG (H+L), 2 mg/mL0.5 mL
92221CF750 Protein Labeling Kit 3 labelings
92142CF750 succinimidyl ester1 umole
20277-1CF770 Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL50 uL
20277CF770 Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL0.25 mL
20242-1CF770 Donkey Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, 2 mg/mL50 uL
20242CF770 Donkey Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, 2 mg/mL0.25 mL
20279-1CF770 donkey anti-human IgG(H+L), highly cross-adsorbed, 2mg/mL50 uL
20279CF770 donkey anti-human IgG(H+L), highly cross-adsorbed, 2mg/mL0.25 mL
20288-1CF770 goat anti-human IgG(H+L), highly cross-adsorbed, 2mg/mL50 uL
20288CF770 goat anti-human IgG(H+L), highly cross-adsorbed, 2mg/mL0.25 mL
20077CF770 Goat Anti-Mouse IgG (H+L) (min X Human, Bovine, Horse, Rabbit, and Swine), 2 mg/mL0.25 mL
20077-1CF770 goat anti-mouse IgG(H+L), highly cross-adsorbed, 2 mg/mL50 uL
20254CF770 Goat anti-mouse IgG1, (γ1), 2 mg/mL0.25 mL
20264CF770 Goat anti-mouse IgG2a, (γ2a), 2mg/mL0.25 mL
20274CF770 Goat anti-mouse IgG2b, (γ2b), 2mg/mL0.25 mL
20078CF770 Goat Anti-Rabbit IgG (H+L) (min X Human, Mouse, and Rat), 2 mg/mL0.25 mL
20078-1CF770 goat anti-rabbit IgG(H+L), highly cross-adsorbed, 2 mg/mL50 uL
20220CF770 monoclonal mouse anti-GFP IgG, 1 mg/mL0.1 mL
20244-1CF770 Rabbit Anti-Guinea Pig IgG (H+L), 2 mg/mL50 uL
20244CF770 Rabbit Anti-Guinea Pig IgG (H+L), 2 mg/mL0.5 mL
92222CF770 SE protein labeling kit3 labelings
92150CF770 succinimidyl ester1 umole
92155CF790 succinimidyl ester0.25 umole