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 제품안내  형광시약   Protein Labeling  CF™ Dyes

CF™680 and CF™680R Dyes

CF680은 같은 파장대의 dye중 밝기가 가장 밝으며, 큰 분자량에 레이블시 사용하는 dye입니다. CF680R은 형광 지속성이 좋으며 핵산이나 작은 분자량의 molecular 레이블시 적합합니다.

Biotium
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    특징
    CF™680 CF™680R
    • 680nm파장대의 dye중 밝기가 가장 밝습니다.
    • Immunostaining시 백그라운드가 가장 깨끗합니다.
    • 물에 매우 잘 녹으며, pH에 민감하지 않습니다.
    • 680nm파장대의 dye중 형광이 가장 오래 지속됩니다.
    • 핵산이나 작은 분자량의 molecular의 레이블에 적합합니다.
    • 물에 매우 잘 녹으며, pH에 민감하지 않습니다.
    사양
    CF™680 CF™680R
    • Abs/Em Maxima: 681/698 nm
    • Extinction coefficient: 210,000
    • Molecular weight: 3241
    • Direct replacement for: Alexa Fluor® 680, Cy™5.5
    • Abs/Em Maxima: 680/701 nm
    • Extinction coefficient: 140,000
    • Molecular weight: 912
    • Direct replacement for: Alexa Fluor® 680, Cy™5.5
    적용
    Figure 1. Absorption and emission spectra of CF™680 and CF™680R conjugated to goat anti-mouse IgG, respectively, in PBS.
    Figure 2. Jurkat cells were stained with isotype or mouse anti-human intracellular CD3 antibody followed by 1 mg of goat anti-mouse IgG conjugated with Cy™5.5, AlexaFluor® 680, CF™680 or DyLight™680. Fluorescence was detected by a BD FACS Calibur in the FL4 channel. The bars represent the signal-to-noise ratio of CD3 positive fluorescence to isotype using similar degrees of labeling (DOL).
    Figure 3. Photostability comparison by microscopy. Jurkat cells were fixed, permeabilized and stained with rabbit anti-CD3 (Abcam) followed by CF™680R (Biotium), AlexaFluor® 680 (Invitrogen) or Cy™5.5 (GE Healthcare) goat anti-rabbit IgG conjugates. Cells were imaged using an Olympus mercury arc lamp microscope equipped with a Cy5 filter set and CCD camera. Images were taken at t=0, 1 min and 5 min.
    Figure 4. Photostability comparison by microscopy. Jurkat cells were fixed, permeabilized and stained with rabbit anti-CD3 (Abcam) followed by CF™680R, CF™F680, Cy™5.5 or AlexaFluor® 680 (AF680) goat anti-mouse IgG conjugates. Cells were imaged using an Olympus mercury arc lamp microscope equipped with a Cy5 filter set and CCD camera. The graph illustrates the relative fluorescence intensities of sequential images taken every 10 seconds for 5 minutes.
    Figure 5. HeLa cells were stained with mouse a-tubulin antibody followed by CF™680 goat anti-mouse IgG. Images were captured using an Olympus mercury arc lamp microscope equipped with a Cy5 filter set, CCD camera and ImagePro Express software.
    Figure 6. Near-IR CF™680 and CF™770 for two-color Western blotting. Two-fold dilutions of HeLa cell lysate were run on an acrylamde gel, transferred to a nitrocellulose membrane and probed with mouse alpha-tubulin and rabbit COX IV primary antibodies followed by goat antimouse CF™770 or IRDye™ 800 (green) and goat anti-rabbit CF™680 or IRDye™680 (red) at the same final concentrations. After probing, membranes were dried and scanned using an Odyssey® infrared imaging system (Li-Cor Biosciences). Quantitation of the bands showed approximately a 3.5-fold higher fluorescence intensity of CF dyes compared to the respective IRDye™ secondary antibodies (Li-Cor).
주문정보(Ordering Information)
CATNUM PRODUCT SIZE
29007Annexin V, CF680 conjugate, lyophilized25 ug
20292Bovine serum albumin, CF680 conjugate5x1 mg
20060-1CF680 donkey anti-goat IgG(H+L), highly cross-adsorbed, 2 mg/mL50 uL
20060CF680 donkey anti-goat IgG(H+L), highly cross-adsorbed, 2 mg/mL0.25 mL
20241-1CF680 Donkey Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, 2 mg/mL50uL
20241CF680 Donkey Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, 2 mg/mL0.25ml
20278-1CF680 donkey anti-human IgG(H+L), highly cross-adsorbed, 2mg/mL50 uL
20278CF680 donkey anti-human IgG(H+L), highly cross-adsorbed, 2mg/mL0.25 mL
20062-1CF680 donkey anti-sheep IgG(H+L), highly cross-adsorbed, 2 mg/mL50 uL
20062CF680 donkey anti-sheep IgG(H+L), highly cross-adsorbed, 2 mg/mL0.5mL
20287-1CF680 goat anti-human IgG(H+L), highly cross-adsorbed, 2mg/mL50 uL
20287CF680 goat anti-human IgG(H+L), highly cross-adsorbed, 2mg/mL0.25 mL
20065CF680 Goat Anti-Mouse IgG (H+L) (min X Human, Bovine, Horse, Rabbit, and Swine), 2 mg/mL0.25 mL
20065-1CF680 goat anti-mouse IgG(H+L), highly cross-adsorbed, 2 mg/mL50 uL
20253CF680 Goat anti-mouse IgG1, (γ1), 2 mg/mL0.25 mL
20263CF680 Goat anti-mouse IgG2a, (γ2a), 2mg/mL0.25 mL
20273CF680 Goat anti-mouse IgG2b, (γ2b), 2mg/mL0.25 mL
20067CF680 Goat Anti-Rabbit IgG (H+L) (min X Human, Mouse, and Rat), 2 mg/mL0.25 mL
20068CF680 goat anti-rabbit IgG(H+L), 2 mg/mL0.5mL
20067-1CF680 goat anti-rabbit IgG(H+L), highly cross-adsorbed, 2 mg/mL50 uL
20069-1CF680 Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL50 uL
20069CF680 goat anti-rat IgG(H+L), highly cross-adsorbed, 2 mg/mL0.5mL
92029CF680 maleimide1 umole
20219CF680 monoclonal mouse anti-GFP IgG, 1 mg/mL0.1 mL
92220CF680 Protein Labeling Kit 3 labelings
20068-1CF680 Rabbit Anti-Goat IgG (H+L), 2 mg/mL50 uL
20243-1CF680 Rabbit Anti-Guinea Pig IgG (H+L), 2 mg/mL50 uL
20243CF680 Rabbit Anti-Guinea Pig IgG (H+L), 2 mg/mL0.5 mL
20061-1CF680 rabbit anti-mouse IgG(H+L), highly cross-adsorbed, 2 mg/mL50 uL
20061CF680 rabbit anti-mouse IgG(H+L), highly cross-adsorbed, 2 mg/mL0.5mL
92139CF680 succinimidyl ester1 umole
20196-1CF680R Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL50 uL
20196CF680R Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL0.25 mL
20194-1CF680R Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL50uL
20194CF680R Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL0.25ml
20195-1CF680R Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL50 uL
20195CF680R Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL0.25 mL
20192-1CF680R Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL50uL
20192CF680R Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL0.25ml
20193-1CF680R Goat anti-rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL50uL
20193CF680R Goat anti-rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL0.25ml
92032CF680R maleimide1 umole
92226CF680R SE protein labeling kit3 labelings
92107CF680R succinimidyl ester1 umole
92283Mix-n-Stain CF680R antibody labeling kit, 1X(5-20ug) labeling1 labeling
00048Phalloidin, CF680R conjugate300 U
29025-1Wheat Germ Agglutinin, CF680R conjugate1mg
29025Wheat Germ Agglutinin, CF680R conjugate5x1mg