• Home
  • About us
  • Log-In
  • Join Us
  • Sitemap
  • Recruit
제품/실험 Q&A
자주묻는질문
자유게시판
실험기법
자료신청
논문자료
클레임 접수
기기 A/S 접수
클레임 진행조회
 제품안내  형광시약   Protein Labeling  CF™ Dyes

CF™660C and CF™660R Dyes

CF 660C와 660R은 둘 다 Alexa Fluor 660보다 더 밝으며, 660C는 단백질 conjugation시 밝기가 높으며, 660R은 형광 지속성이 뛰어납니다.

Biotium
제품 문의하기
인쇄하기

    특징
    CF™660C CF™660R
    • Alexa Fluor 660 보다 더 밝습니다.
    • Alexa Fluor 660 보다 더 형광이 오랫동안 지속됩니다.
    • 물에 용해도가 뛰어납니다.
    • Alexa Fluor 660 보다 더 밝습니다.
    • confocal microscopy 사용에 적합하며 660 nm에서 형광 지속성이 가장 뛰어납니다.
    • 물에 용해도가 뛰어납니다.
    사양
    CF™660C CF™660R
    • Abs/Em Maxima: 667/685 nm
    • Extinction coefficient: 200,000
    • Molecular weight: 3112
    • Flow cytometry laser line: 633 or 635 nm
    • Microscopy laser line: 633 or 635 nm
    • Direct replacement for: Alexa Fluor® 660, Allophycocyanin (APC)
    • Abs/Em Maxima: 663/682 nm
    • Extinction coefficient: 100,000
    • Molecular weight: 888
    • Flow cytometry laser line: 633 or 635 nm
    • Microscopy laser line: 633 or 635 nm
    • Direct replacement for: Alexa Fluor® 660, Allophycocyanin (APC)
    적용
    Figure 1. Absorption and emission spectra of CF™660C and CF™660R conjugated to goat anti-mouse IgG, respectively, in PBS.
    Figure 2. Relative fluorescence of CF™660C-, CF™660R and Alexa Fluor® 660-goat anti-mouse conjugates as a function of number of dye per protein (i.e., degree of labeling). Fluorescence was measured at each dye’s emission maximum in PBS using 633 nm excitation.
    Figure 3. Jurkat cells were fixed, permeabilized and stained with rabbit anti-CD3 (Abcam) followed by CF™660C (Biotium) or AlexaFluor® 660 (Invitrogen) goat anti-rabbit IgG conjugates. Cells were imaged using an Olympus mercury arc lamp microscope equipped with a Cy5 filter set and CCD camera. Images were taken at t=0 and 10 second