항체에 대한 conjugation효과가 높으며, 백그라운드가 가장 낮은 cyanine-based 원적외선 형광 dye입니다.
Alexa Fluor 647, DyLight™ 649, Cy™ 5에 비해 conjugation효과가 높으며, 백그라운드가 가장 낮습니다.
물에 잘 녹으며, pH에 민감하지 않습니다.
Abs/Em Maxima: 650/665 nm
Extinction coefficient: 240,000
Molecular weight: ~836
Flow cytometry laser line: 633 or 635 nm
Microscopy laser line: 633 or 635 nm
Direct replacement for: Cy™5, Alexa Fluor® 647 and DyLight™ 649
Figure 1. Absorption and emission spectra of CF™647 conjugated to goat anti-mouse IgG in PBS
Figure 2. Jurkat cells were treated with staurosporine to induce apoptosis and stained with NucView™488 (live cell caspase-3 detection) and CF™647 Annexin-V. CF™647 Annexin-V staining is pseudocolored magenta.
Figure 3. Jurkat cells were fixed, permeabilized and stained with intracellular mouse anti-human CD3 followed by CF™647 (Biotium), AlexaFluor® 647 (Invitrogen) or other commercially available goat anti-mouse IgG conjugates. Stained cells were analyzed on a BD FACS Calibur in the FL4 channel. (A) The bars represent the relative geometric means of the cell population. Background was determined by secondary antibody staining alone. (B) The signal-to-noise ratio of the geometric means from A.