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 제품안내  형광시약   Protein Labeling  CF™ Dyes

CF™647 Dye

항체에 대한 conjugation효과가 높으며, 백그라운드가 가장 낮은 cyanine-based 원적외선 형광 dye입니다.

Biotium
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    특징
  • Alexa Fluor 647, DyLight™ 649, Cy™ 5에 비해 conjugation효과가 높으며, 백그라운드가 가장 낮습니다.
  • 밝습니다.
  • 물에 잘 녹으며, pH에 민감하지 않습니다.
    사양
  • Abs/Em Maxima: 650/665 nm
  • Extinction coefficient: 240,000
  • Molecular weight: ~836
  • Flow cytometry laser line: 633 or 635 nm
  • Microscopy laser line: 633 or 635 nm
  • Direct replacement for: Cy™5, Alexa Fluor® 647 and DyLight™ 649
    적용
    Figure 1. Absorption and emission spectra of CF™647 conjugated to goat anti-mouse IgG in PBS
    Figure 2. Jurkat cells were treated with staurosporine to induce apoptosis and stained with NucView™488 (live cell caspase-3 detection) and CF™647 Annexin-V. CF™647 Annexin-V staining is pseudocolored magenta.
    Figure 3. Jurkat cells were fixed, permeabilized and stained with intracellular mouse anti-human CD3 followed by CF™647 (Biotium), AlexaFluor® 647 (Invitrogen) or other commercially available goat anti-mouse IgG conjugates. Stained cells were analyzed on a BD FACS Calibur in the FL4 channel. (A) The bars represent the relative geometric means of the cell population. Background was determined by secondary antibody staining alone. (B) The signal-to-noise ratio of the geometric means from A.
주문정보(Ordering Information)
CATNUM PRODUCT SIZE
29003Annexin V, CF 647 conjugate0.5 mL
20042CF 647 F(ab')2 fragment of goat anti-mouse IgG(H+L), 2 mg/mL0.25 mL
20045CF 647 F(ab')2 fragment of goat anti-rabbit IgG(H+L), 2 mg/mL0.25 mL
20040CF 647 goat anti-mouse IgG(H+L), 2mg/mL0.5 mL
20043CF 647 goat anti-rabbit IgG(H+L), 2mg/mL0.5 mL
00041CF 647 phalloidin300 U
20048CF647 Donkey Anti-Goat IgG (H+L) (min X Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, and Rat), 2 mg/mL0.5 mL
20048-1CF647 donkey anti-goat IgG(H+L), highly cross-adsorbed, 2 mg/mL50 uL
20046CF647 Donkey Anti-Mouse IgG (H+L) (min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Rabbit, and Sheep), 2 mg/mL0.5 mL
20046-1CF647 donkey anti-mouse IgG(H+L), highly cross-adsorbed, 2 mg/mL50 uL
20047CF647 Donkey Anti-Rabbit IgG (H+L) (min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rat, and Sheep), 2 mg/mL0.5 mL
20047-1CF647 donkey anti-rabbit IgG(H+L), highly cross-adsorbed, 2mg/mL50 uL
20284-1CF647 donkey anti-sheep IgG(H+L), highly cross-adsorbed, 2 mg/mL50uL
20284CF647 donkey anti-sheep IgG(H+L), highly cross-adsorbed, 2 mg/mL0.5ML
20044-1CF647 goat anti-chicken IgY(H+L), highly cross-adsorbed, 2 mg/mL50 uL
20044CF647 goat anti-chicken IgY(H+L), highly cross-adsorbed, 2 mg/mL0.5 mL
20041-1CF647 Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL50 uL
20041CF647 goat anti-guinea pig IgG(H+L), 2 mg/mL0.5 mL
20280-1CF647 goat anti-human IgG(H+L), highly cross-adsorbed, 2 mg/mL50 uL
20280CF647 goat anti-human IgG(H+L), highly cross-adsorbed, 2 mg/mL0.5mL
20040-1CF647 Goat anti-mouse IgG (H+L), 2 mg/mL50 uL
20281-1CF647 goat anti-mouse IgG(H+L), highly cross-adsorbed, 2 mg/mL50 uL
20281CF647 goat anti-mouse IgG(H+L), highly cross-adsorbed, 2 mg/mL0.5mL
20252CF647 Goat anti-mouse IgG1, (γ1), 2 mg/mL0.25 mL
20262CF647 Goat anti-mouse IgG2a, (γ2a), 2mg/mL0.25 mL
20272CF647 Goat anti-mouse IgG2b, (γ2b), 2mg/mL0.25 mL
20043-1CF647 Goat Anti-Rabbit IgG (H+L), 2 mg/mL50 uL
20282-1CF647 goat anti-rabbit IgG(H+L), highly cross-adsorbed, 2 mg/mL50 uL
20282CF647 goat anti-rabbit IgG(H+L), highly cross-adsorbed, 2 mg/mL0.5mL
20283-1CF647 goat anti-rat IgG(H+L), highly cross-adsorbed, 2 mg/mL50 uL
20283CF647 goat anti-rat IgG(H+L), highly cross-adsorbed, 2 mg/mL0.5mL
20286-1CF647 Goat Anti-Swine IgG (H+L), 2 mg/mL50 uL
20286CF647 goat anti-swine IgG(H+L), 2 mg/mL0.5mL
92218CF647 Protein Labeling Kit 3 labelings
20049-1CF647 Rabbit Anti-Goat IgG (H+L), 2 mg/mL50 uL
20049CF647 rabbit anti-goat IgG(H+L), 2 mg/mL0.5 mL
20285-1CF647 rabbit anti-mouse IgG(H+L), highly cross-adsorbed, 2 mg/mL50 uL
20285CF647 rabbit anti-mouse IgG(H+L), highly cross-adsorbed, 2 mg/mL0.5mL
92135CF647, succinimidyl ester1 umole