640nm laser 사용시 가장 밝은 형광을 내는 rhodamine-based 원적외선 형광 dye입니다.
Cy5와의 같은 파장대의 형광 dye중 가장 밝습니다.
단백질의 cojugation yield가 높습니다.
물에 매우 잘 녹으며, pH에 민감하지 않습니다.
Abs/Em Maxima: 642/662 nm
Extinction coefficient: 105,000
Molecular weight: 832
Flow cytometry laser line: 633, 635 or 640 nm
Microscopy laser line: 633, 635 or 640 nm
Direct replacement for: Cy™5, Alexa Fluor® 647 and ATTO™ 647N
Figure 1.Absorption and emission spectra of CF™640R and Cy™5 conjugated to goat anti-mouse IgG in PBS
Figure 2.Jurkat cells were stained with intracellular CD3 followed by goat anti-mouse IgG conjugates. Background staining was determined by secondary antibody staining alone. Fluorescence was analyzed on a BD FACS Calibur in the FL4 channel. The bars represent the relative fluorescence of the geometric means of the population of cells.
Figure 3.Photostability comparison between CF™640R and Atto™647N, a far-red dye frequently used in single-molecule imaging. Dye solutions at 0.5 mM in PBS were continuously exposed using a Cy™ 5 filter set on a mercury arc lamp microscope. Images were captured every 15 seconds, and mean fluorescence intensity of each image was calculated using Image Pro Express software.