Alexa Fluor 488만큼의 밝기를 가지며, Alexa Fluor 488 보다 spill-over현상이 적은 green 형광 dye입니다.
charge를 적게 띄어 항체 cojugation시 non-specicific binding을 감소시킵니다.
Alexa Fluor 488 보다 red channel에서 spill-over가 적게 나타납니다.
형광이 매우 오랫동안 지속됩니다.
매우 물에 잘 녹으며, pH에 민감하지 않습니다.
reactive dye의 레이블링 효과가 뛰어납니다.
Abs/Em Maxima: 490/515 nm
Extinction coefficient: 70,000
Molecular weight: ~910
Flow cytometry laser line: 488 nm
Microscopy laser line: 488 nm
Direct replacement for: Alexa Fluor® 488, DyLight™488, FITC, FAM, Cy™2
Figure 1. Absorption and emission spectra of CF™488A (blue), Alexa Fluor® 488 (green) and FITC (red) conjugated to goat anti-mouse IgG in PBS.
Figure 2. Jurkat cells were stained with 0.5 ug intracellular CD3 or isotype control (BD Biosciences) followed by 1 ug goat anti-mouse IgG conjugates from the manufacturer’s shown above. Fluorescence was analyzed on a Beckman Coulter FC-500. The bars represent the median values of the gated population of cells. The histogram depicts CF488A (green), AlexaFluor488 (blue), DyLight488 Pierce (purple) and DyLight488 KPL (red) stained cells
Figure 3. Cryosections (6 um) of human control heart sections stained with anti-fibronectin followed by goat anti-rabbit IgG labeled with CF™488A, Cy™2 and Alexa Fluor® 488, respectively. Courtesy of Dr. Sawa Kostin at Max-Planck-Institute fur Herz-und Lungenforschung (W.G. Kerckhoff-Institut) in Bad Nauheim (Hessen)-Germany