Alexa Fluor 405 보다 훨씬 밝은 blue 형광 dye 입니다.
- Alexa Fluor 405 보다 훨씬 더 밝습니다.
- 물에 매우 잘 녹습니다.
- pH에 민감하지 않습니다.
- Abs/Em Maxima: 404/431 nm
- Extinction coefficient: 33,000
- Molecular weight: 1,169
- Flow cytometry laser line: 405 nm
- Microscopy laser line: 405 nm
- Direct replacement for: Alexa Fluor® 405, Cascade Blue® dye, DyLight™ 405
Figure 1. Absorption and emission spectra of CF™405S and Alexa Fluor® 405 conjugated to goat anti-mouse IgG in PBS. The shaded area represents the wavelength window of the 450/50 filter on a BD LSR II flow cytometer.
Figure 2. Jurkat cells were stained with intracellular CD3 or isotype control followed by goat anti-mouse IgG conjugates. Fluorescence was analyzed on a BD LSRII (405 laser, Em 450/50 nm). The bars represent the relative fluorescence of the geometric means of the population of cells.
Figure 3. HeLa cells stained with mouse-Golgin 97 and rabbit COX IV followed by goat anti-mouse CF405S (blue Golgi), goat anti-rabbit CF488A (green mitochondria) and CF555 phalloidin (red F-actin).
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