전형적인 AMCA dye와 유사한 파장대를 가지는 blue 형광 dye로, AMCA에 비해 더 물에 잘녹으며, 빛이 오랫동안 지속됩니다.
350 nm excitation에서 밝기가 가장 밝습니다.
물에 매우 잘 녹습니다.
pH에 민감하지 않습니다.
Abs/Em Maxima: 347/448 nm
Extinction coefficient: 18,000
Molecular weight: 496
Flow cytometry laser line: UV
Microscopy laser line: UV nm
Direct replacement for: Alexa Fluor® 350, AMCA
Figure 1. Absorption and emission spectra of CF™350 conjugated to goat anti-mouse IgG in PBS.
Figure 2. Relative fluorescence of CF™350- and Alexa Fluor® 350-goat anti-mouse conjugates as a function of number of dye per protein (i.e., degree of labeling). Fluorescence was measured at each dye’s emission maximum in PBS using 350 nm excitation.
Figure 3. HeLa cells were fixed, permeabilized and stained with mouse anti-alpha-tubulin followed by CF350 goat anti-mouse IgG. Image was taken on an Olympus mercury arc lamp microscope.