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 제품안내  분자생물학   Viral Expression  Lentiviral Expression

ViraBind™ Lentivirus Concentration and Purification Kit

초고속 원심분리방법을 이용하지 않고 lentivirus pellet으로 부터 고농도, 고순도의 lentivirus를 농축 및 정제하는 제품입니다.

Cell Biolabs
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특징
  • Lentivirus를 최대 500배까지 농축할 수 있습니다. (PLUS Kit의 경우 10^8 to 10^9 TU/mL까지 농축할 수 있습니다.)
  • 초고속 원심분리를 이용하여 3~5시간 소요되는 방법보다 더 높은 순도의 virus를 얻을 수 있습니다.
  • filter를 이용하는 방법에 비해 더 많은 양을 처리 할 수 있습니다.
  • in vivo 연구에도 적합합니다.
원리
ViraBind™ Lentivirus Concentraion and Purification Kit 원리
구성품
  • Lentivirus Concentration and Purification Kit
    • ViraBind™ Lentivirus Reagent A (100X)
    • ViraBind™ Lentivirus Reagent B (100X)
    • Purification Columns
    • Centrifugal Concentrators
    • Purification Buffer
  • PLUS Lentivirus Concentration and Purification Kit
    • ViraBind™ Lentivirus Reagent A (100X)
    • ViraBind™ Lentivirus Reagent B (100X)
    • Dialysis Devices
    • Centrifugal Concentrators
    • Purification Buffer
적용
Figure 1. Electrophoretic Profile of Purified GFP lentivirus. 50 mL of GFP lentiviral supernatant was concentrated and purified according to the described Assay Instructions. Lentiviral Supernatant (A), Virus Pellet (B) and Purified Virus Fraction (C) were analyzed on SDS-PAGE. Proteins were visualized by Commassie blue stain (left) and silver stain (right). The VSV-G envelope protein and p24 coat protein (the most abundant proteins in the vector) are indicated.
Figure 2. Free p24 does not complex with ViraBind™. Recombinant p24 diluted in culture medium was treated with ViraBind™ Lentivirus Reagents. The amount of p24 in supernatant and pellet was measured by p24 ELISA (Cat# VPK-107, Lentivirus Associated HIV p24 ELISA).
Figure 3. Vector Yield Determined by p24 ELISA. GFP lentiviral supernatant was concentrated and purified according to the Assay Instructions. Purification fractions of Lentiviral Supernatant (A), Virus Pellet (B) and Purified Virus Fraction (C) were used to infect 293 cells with GFP expression determined after 72 hr. The p24 titer of each fraction was determined by p24 ELISA (Cat.# VPK-107, Lentivirus Associated HIV p24 ELISA).