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 제품안내  면역학   Immunoassays  Cytokine Research

Recombinant Proteins Produced in Plants

박테리아가 아닌, 식물을 이용하여 제조된 recombinant protein 및 항체를 소개합니다. Mammalian contaminants를 갖고 있지 않으므로, 100% animal free system 이며, 이는 cell biology 실험에 있어 clean background를 보장합니다.

Agrenvec
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    특징
  • 100% Animal-free plus Serum-free:
    동물유래 조성등으로 기인할 지 모르는 contamination 우려가 없어 cell culture 연구에 효과적입니다.
  • Endotoxin-free plus Antibiotic-free:
    박테리아에서 생성되는 재조합단백질에 비해 endotoxin level이 낮으므로 pyrogenic, inflammatory activity를 피할 수 있습니다.
  • Complex proteins with post-translational modifications:
    3D structure로 생성되므로, mammalian cell cultures 환경과 비슷합니다.
  • No inclusion bodies:
    박테리아와 달리 inclusion body가 생성되지 않으므로, denature, renature등의 과정이 필요없어, activity loss가 없습니다.
  • 100% Functional:
    박테리아 유래 단백질보다 activity가 월등히 좋습니다.
  • Clean Antibodies:
    plant 유래 antigen으로 생성된 항체들은 mammalian background signal로부터 안전합니다.
    Specification
  • Recombinant Antigen
    • 100% Animal free
    • 100% Active
    • ≥ 95% pure
    • Cell culture grade
    • High complex 3D structure
  • Antibodies
    • No vertebrate background signal
    • 1 hour primary antibody incubation in Western Blot Assay
    Plant virus vector를 이용한 기술의 장점
  • small genome size를 이용하므로 조작이 쉽다.
  • RNA를 잎에 접종함으로써 replication level이 높다.
  • RT-PCR과 ELISA로 단계별 QC를 진행할 수 있다.
  • transient expression에 의해 단백질을 빨리 생성할 수 있다.
  • 모든 cell에서 homogeneous production을 가진다.
  • Tagging이 가능하므로 단백질 정제가 더 쉽다.
    제조 과정
  • Clone cDNA into the vector
    We select the protein of interest, generate the cDNA and clone it
    Clone cDNA into the vector
  • Mechanical inoculation of the vector in plant leaves
    2.a.Localized infection with production verctor
    2.b.Cell-to-cell movement
    2.c.Cell-to-cell movement, systemic, spread,High yield protein production
    Mechanical inoculation of the vector in plant leaves
  • Protein purification by FPLC
    1,2,3 Different high complex Recombinant proteins
    - 100% Animal free
    - 100% Active
    - ≥ 97% Pure
    Protein purification by FPLC
  • Rabbit polyclonal Antibody production
    1,2,3 Dirrerent Aitibodies (Clean Antibodies)
    - Plant produced proteins lead to polyclonal antibodies having no cross reaction with other mammalian protein
    Rabbit polyclonal Antibody production
  • Final products
    Recombinant proteins and Antibodies
    Final products
주문정보(Ordering Information)
CATNUM PRODUCT SIZE
RF0016IgG Rabbit Polyclonal to Human ACTIVIN A100ug
RF0016-1IgG Rabbit Polyclonal to Human ACTIVIN A1mg
RF0017IgG Rabbit Polyclonal to Human BMP 7100ug
RF0017-1IgG Rabbit Polyclonal to Human BMP 71mg
RF0014IgG Rabbit Polyclonal to Human HGH (Somatotropin)100ug
RF0014-1IgG Rabbit Polyclonal to Human HGH (Somatotropin)1mg
RF0013IgG Rabbit Polyclonal to Human Interferon α 2a100ug
RF0013-1IgG Rabbit Polyclonal to Human Interferon α 2a1mg
RF0020-1IgG Rabbit Polyclonal to Human LXR β1mg
RF0020IgG Rabbit Polyclonal to Human LXR β100ug
RF0022-1IgG Rabbit Polyclonal to Human Myostatin1mg
RF0022IgG Rabbit Polyclonal to Human Myostatin100ug
RF0012-1IgG Rabbit Polyclonal to Human TFPI-21mg
RF0012IgG Rabbit Polyclonal to Human TFPI-2100ug
RF0015IgG Rabbit Polyclonal to Human TGF β2100ug
RF0015-1IgG Rabbit Polyclonal to Human TGF β21mg
RF0018IgG Rabbit Polyclonal to Human TGF β3100ug
RF0018-1IgG Rabbit Polyclonal to Human TGF β31mg
RF009-5r Human ACTIVIN A5ug
RF009-10r Human ACTIVIN A10ug
RF009-1r Human ACTIVIN A1mg
RF0010-5r Human ACTIVIN A Active5ug
RF0010-10r Human ACTIVIN A Active10ug
RF0010-1r Human ACTIVIN A Active1mg
RF0019-1r Human LXR 1mg
RF0019-50r Human LXR β-LBD50
RF0019-5r Human LXR β-LBD5ug
RF0019-25r Human LXR β-LBD25ug
RF0019-100r Human LXR β-LBD100ug
RF0019-10r Human LXR β-LBD10ug
RF007-1r Human TFPI-2 domain 11mg
RF001-5r Human TGF β 25ug
RF001-2.5r Human TGF β 22.5ug
RF001-1r Human TGF β 21mg
RF003-5r Human TGF β 2 Active5ug
RF003-2.5r Human TGF β 2 Active2.5ug
RF003-1r Human TGF β 2 Active1mg
RF002-5r Human TGF β 35ug
RF002-2.5r Human TGF β 32.5ug
RF002-1r Human TGF β 31mg
RF004-5r Human TGF β 3 Active5ug
RF004-2.5r Human TGF β 3 Active2.5ug
RF004-1r Human TGF β 3 Active1mg