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 Á¦Ç°¾È³»  ¼¼Æ÷¹è¾ç   Stem Cell  iPSC Generation

Platinum Retroviral Expression

iPS cell (induced pluripotent stem cells) generationÀ» À§ÇÑ Retroviral expression kit À¸·Î, ¿ì¼öÇÑ packaging cell line°ú ´ÜÀÏ Çö󽺹̵å transfectionÀ¸·Î °í¿ª°¡(High-titer) ¹ÙÀÌ·¯½º¸¦ »ý»êÇÏ´Â vector ±â¼úÀ» ÅëÇÕÇϰí ÀÖ½À´Ï´Ù.

Cell Biolabs
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    Platinum Retroviral Expression Systems for Stem Cells
  • ÀϽà ¹ßÇö Æò±Õ ¿ª°¡ 107 infectious units/mlÀ¸·Î ¸¹Àº ¹ÙÀÌ·¯½º¸¦ ¼öÈ®ÇÒ ¼ö ÀÖ½À´Ï´Ù.
  • ¼±ÅÃÇÑ drug¿¡ µû¶ó ÃÖ´ë 4°³¿ù ¹ßÇöµÇ¾î Áö¼ÓÀûÀ¸·Î ¾ÈÁ¤¼ºÀ» À¯ÁöÇÕ´Ï´Ù.
  • ¾î¶² target host species¿Íµµ »ç¿ëÇÒ ¼ö ÀÖµµ·Ï 3°¡ÁöÀÇ packaging cell lineÀ» ¼±ÅÃÇÒ ¼ö ÀÖ½À´Ï´Ù.
  • ES/EC/HSC ¿¬±¸¿¡ »ç¿ëÇÒ ¼ö ÀÖµµ·Ï µðÀÚÀεǾú½À´Ï´Ù.
Suitability of Retroviral Packaging Cell Lines by Host
- Plat-A Cells Plat-E Cells Plat-GP Cells
(Amphotropic) (Ecotropic) (Pantropic*)
Human +++ N.S. +++
Mouse +++ +++ +++
Rat +++ +++ +++
Monkey +++ N.S. +++
Cat +++ N.S. +++
Dog +++ N.S. +++
Hamster + N.S. +++
Bird N.S. N.S. +++
Fish N.S. N.S. +++
Frog N.S. N.S. +++
Insect N.S. N.S. +++
Mollusk N.S. N.S. +++
*Retrovirus must be packaged with a pantropic envelope protein such as VSVG
N.S. = Not Suitable
Retrovirus Production in Packaging Cells and Virus Tropism