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 Á¦Ç°¾È³»  Çü±¤½Ã¾à   Nucleic Acid Stain

RedDot Far-red nuclear counterstains

CellÀÇ nuclei¸¦ ¿°»öÇÏ´Â counterstainÀ¸·Î, live cell¿°»öÀ» À§ÇÑ RedDot type 1°ú dead cell ¿°»öÀ» À§ÇÑ RedDot type 2·Î Á¦°øµË´Ï´Ù.

Biotium
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  • RedDot dye´Â specificity°¡ ÁÁ°í stableÇÏ¿© nuclear staining¿¡ ÀÌ»óÀûÀÎ ±ÙÀû»ö±¤ (far-red) dye ÀÔ´Ï´Ù.
  • ±âÁ¸ÀÇ DAPI, Draq¢â5, Draq¢â7µîÀÇ counterstaining dye¿¡ ºñÇØ, ¿­°ú ºû¿¡ ¾ÈÁ¤ÇÕ´Ï´Ù.
  • 568, 594, 633, 635, 647 nm µîÀÇ ´Ù¾çÇÑ laser line¿¡ ÀÇÇØ excitationµÉ ¼ö ÀÖ½À´Ï´Ù.
  • Immunocytochemistry ¹× high-content screening assaysµî¿¡ È¿°úÀûÀ¸·Î »ç¿ëµË´Ï´Ù.
  • FACS½Ã, ´Ù¾çÇÑ Çü±¤À» ¶óº§¸µÇÑ 1Â÷Ç×ü·Î cell sub-populations sorting½Ã µµ¿òÀÌ µË´Ï´Ù.
  • Cy¢â5 filter·Î ¸®µùÇÒ ¼ö ÀÖ½À´Ï´Ù.
    Á¾·ù
  • RedDot¢â1
    • Far-red cell membrane-permeable nuclear dye·Î Live cellÀÇ ÇÙÀ» ¿°»öÇÕ´Ï´Ù. (fixed and permeabilized cells¿¡´Â ÀûÇÕÇÏÁö ¾Ê½À´Ï´Ù.)
    • Suspension°ú adherent cellsÀ» ¸ðµÎ ¿°»öÇÒ ¼ö ÀÖ½À´Ï´Ù.
    • Abs/Em with DNA: 662/694 nm (¾à 665nm¿¡¼­ Çü±¤)
    • ÀüÅëÀûÀÎ nuclear counterstainÀÎ DAPI¿¡ ºñÇØ ÈξÀ Çü±¤¿¡ ¾ÈÁ¤Çϸç, washing ½ºÅÜÀÌ ÇÊ¿ä¾ø½À´Ï´Ù.
    Figure 1. Absorption and emission spectra of RedDot¢â1 in live Jurkat cells. Absorbance was measured from 250-800 nm and emission scan was performed using 610 nm excitation.
    Figure 2. Live HeLa cells in 1X PBS were stained with 1X RedDot¢â1 dye for 5 minutes at 37¡ÆC. Cells were imaged using a 40X objective on an Olympus mercury arc lamp microscope equipped with a Cy5 filter set, CCD camera and ImagePro Express software.
  • RedDot¢â2
    • Far-red cell membrane-impermeable nuclear dye·Î dead cellÀÇ ÇÙÀ» ¿°»öÇϸç, fixed and permeabilized cells staining¿¡ ÀûÇÕÇÕ´Ï´Ù.
    • Cytoplasmic RNA stainingÀ» ÃÖ¼ÒÈ­Çϱâ À§ÇÑ RNAase 󸮴ܰ谡 ÇÊ¿ä¾ø½À´Ï´Ù.
    • Abs/Em with DNA: 665/695 nm (¾à 665nm¿¡¼­ Çü±¤)
    Figure 1. Absorption and emission spectra of RedDot¢â2 in fixed and permeabilized Jurkat cells. Absorbance was measured from 250-800 nm and emission scan was performed using 610 nm excitation.
    Figure 2. Acetone-fixed frozen section of rat skeletal muscle stained with RedDot¢â2 (red) to stain nuclei and CF¢â488A-phalloidin (green) to stain actin filaments. Images were captured on an Olympus mercury arc lamp microscope using with FITC and Cy¢â5 filter sets.