• Home
  • About us
  • Log-In
  • Join Us
  • Sitemap
  • Recruit
제품/실험 Q&A
자주묻는질문
자유게시판
실험기법
자료신청
논문자료
클레임 접수
기기 A/S 접수
클레임 진행조회
 제품안내  분자생물학   Viral Expression  AAV Expression

AAV Helper Free Packaging & Expression Systems

AAV Helper Free System은 live helper adenovirus 필요없이, vector를 이용하여, 관심 gene을 갖는 AAV(adeno-associated virus)를 생성하는 시스템입니다.

Cell Biolabs
제품 문의하기
인쇄하기

    특징
  • Live helper virus 을 제거함으로써 안전하고 편리한 gene delivery system 입니다.
  • AAV packaging을 위한 adenoviral genes 들은 pHelper plasmid (E2A, E4 and VA RNA)에 의해 제공되거나 293 packaging cells (E1)로부터 공급됩니다.
  • 1-6까지의 native serotypes 및 AAV-DJ 와 AAV-DJ/8 군으로 공급됩니다.
  • AAV-DJ 군은 8가지의 다른 native serotype (NAAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, caprine AAV)을 조합하여 생성된 hybrid capsid를 제공함으로써, 다양한 tissue 와 cell types에서 native serotypes보다 높은 infection 효율을 보입니다.
  • AAV-DJ/8군은 AAV-DJ의 heparin binding domain에 point mutation을 일으킨 제품으로, 이를 통해 생성된 recombinant AAV는 in vivo상에서 heart, brain tissue등에서 native serotypes 8 (AAV-8), 9 (AAV-9)와 유사하게 발현됩니다.
  • Expression vector, GFP control vector, pHelper Vector 만 제공되는 Packaging System 및 전용 expression vector가 함께 제공되는 Expression System 이 있습니다.
    제품의 구성
    Components Packaging System Expression System
    pAAV Vector Yes Yes
    pHelper Vector Yes Yes
    pAAV-GFP Control Vector Yes Yes
    pAAV Expression Vector - Yes
    Figure 1: Viral Gene Delivery using the AAV Helper Free System.
    Figure 1: Viral Gene Delivery using the AAV Helper Free System.
    Figure 2:Production and Transduction of AAV2-GFP. Top Left: 293AAV cells prior to transfection (10X). Top Right: 293AAV cells 48 hours after transfection (10X). Bottom Left: GFP expression in 293AAV cells 48 hours after transfection (10X). Bottom Right: GFP expression in 293AD cells 48 hours after infection (20X).
    Figure 2: Production and Transduction of AAV2-GFP. Top Left: 293AAV cells prior to transfection (10X). Top Right: 293AAV cells 48 hours after transfection (10X). Bottom Left: GFP expression in 293AAV cells 48 hours after transfection (10X). Bottom Right: GFP expression in 293AD cells 48 hours after infection (20X).
    Figure 3: Generation of AAV-DJ through Capsid DNA Family Shuffling.
    Figure 3: Generation of AAV-DJ through Capsid DNA Family Shuffling.
    Relative in vitro Infectivity of AAV Vectors
    Cell Line AAV-1 AAV-2 AAV-3 AAV-4 AAV-5 AAV-6 AAV-8 AAV-9 AAV-DJ AAV-DJ/8
    Huh-7 13 100 2.5 0 0.1 10 0.7 0 500 0.2
    HEK293 25 100 2.5 0.1 0.1 5 0.7 0.1 500 0.3
    HeLa 3 100 2 0.1 6.7 1 0.2 0.1 667 0.2
    HepG2 3 100 16.7 0.3 1.7 5 0.3 ND 1250 0.5
    Hep1A 20 100 0.2 1 0.1 1 0.2 0 400 0.1
    911 17 100 11 0.2 0.1 17 0.1 ND 500 0
    CHO 100 100 14 1.4 333 50 10 1 25000 5
    COS 33 100 33 3.3 5 14 2 0.5 500 0.3
    MeWo 10 100 20 0.3 6.7 10 1 0.2 2857 1
    NIH3T3 10 100 2.9 2.9 0.3 10 0.3 ND 500 0.1
    A549 14 100 20 ND 0.5 10 0.5 0.1 1000 0.1
    HT1180 20 100 10 0.1 0.3 33 0.5 0.1 333 0.2
    Monocytes 1111 100 ND ND 125 1429 ND ND 100 ND
    Immature DC 2500 100 ND ND 222 2857 ND ND 200 ND
    Mature DC 2222 100 ND ND 333 3333 ND ND 100 ND
주문정보(Ordering Information)
CATNUM PRODUCT SIZE
VPK-400-DJAAV-DJ Helper Free Packaging System 1 kit
VPK-400-DJ-8AAV-DJ/8 Helper Free Packaging System 1 kit
VPK-401AAV-1 Helper Free Packaging System1 kit
VPK-402AAV-2 Helper Free Packaging System1 kit
VPK-403AAV-3 Helper Free Packaging System1 kit
VPK-404AAV-4 Helper Free Packaging System1 kit
VPK-405AAV-5 Helper Free Packaging System1 kit
VPK-406AAV-6 Helper Free Packaging System1 kit
VPK-410pAAV-MCS Expression Vector10ug
VPK-410-DJ-8AAV-DJ/8 Helper Free Expression System 1 kit
VPK-410-SER1AAV-1 Helper Free Expression System1 kit
VPK-410-SER2AAV-2 Helper Free Expression System1 kit
VPK-410-SER3AAV-3 Helper Free Expression System1 kit
VPK-410-SER4AAV-4 Helper Free Expression System1 kit
VPK-410-SER5AAV-5 Helper Free Expression System1 kit
VPK-410-SER6AAV-6 Helper Free Expression System1 kit
VPK-415-DJAAV-DJ Helper Free Bicistronic Expression System (Puro)1 kit
VPK-415-DJ-8AAV-DJ/8 Helper Free Bicistronic Expression System (Puro)1 kit
VPK-415-SER1AAV-1 Helper Free Bicistronic Expression System (Puro)1 kit
VPK-415-SER2AAV-2 Helper Free Bicistronic Expression System (Puro)1 kit
VPK-415-SER3AAV-3 Helper Free Bicistronic Expression System (Puro)1 kit
VPK-415-SER4AAV-4 Helper Free Bicistronic Expression System (Puro)1 kit
VPK-415-SER5AAV-5 Helper Free Bicistronic Expression System (Puro)1 kit
VPK-415-SER6AAV-6 Helper Free Bicistronic Expression System (Puro)1 kit
VPK-416-DJAAV-DJ Helper Free Bicistronic Expression System (Neo)1 kit
VPK-416-DJ-8AAV-DJ/8 Helper Free Bicistronic Expression System (Neo)1 kit
VPK-416-SER1AAV-1 Helper Free Bicistronic Expression System (Neo) 1 kit
VPK-416-SER2AAV-2 Helper Free Bicistronic Expression System (Neo)1 kit
VPK-416-SER3AAV-3 Helper Free Bicistronic Expression System (Neo) 1 kit
VPK-416-SER4AAV-4 Helper Free Bicistronic Expression System (Neo) 1 kit
VPK-416-SER5AAV-5 Helper Free Bicistronic Expression System (Neo) 1 kit
VPK-416-SER6AAV-6 Helper Free Bicistronic Expression System (Neo) 1 kit
VPK-417-DJAAV-DJ Helper Free Bicistronic Expression System (Hygro) 1 kit
VPK-417-DJ-8AAV-DJ/8 Helper Free Bicistronic Expression System (Hygro) 1 kit
VPK-417-SER1AAV-1 Helper Free Bicistronic Expression System (Hygro)1 kit
VPK-417-SER2AAV-2 Helper Free Bicistronic Expression System (Hygro)1 kit
VPK-417-SER3AAV-3 Helper Free Bicistronic Expression System (Hygro)1 kit
VPK-417-SER4AAV-4 Helper Free Bicistronic Expression System (Hygro)1 kit
VPK-417-SER5AAV-5 Helper Free Bicistronic Expression System (Hygro)1 kit
VPK-417-SER6AAV-6 Helper Free Bicistronic Expression System (Hygro)1 kit
VPK-418-DJAAV-DJ Helper Free Bicistronic Expression System (GFP) 1 kit
VPK-418-DJ-8AAV-DJ/8 Helper Free Bicistronic Expression System (GFP) 1 kit
VPK-418-SER1AAV-1 Helper Free Bicistronic Expression System (GFP) 1 kit
VPK-418-SER2AAV-2 Helper Free Bicistronic Expression System (GFP)1 kit
VPK-418-SER3AAV-3 Helper Free Bicistronic Expression System (GFP) 1 kit
VPK-418-SER4AAV-4 Helper Free Bicistronic Expression System (GFP) 1 kit
VPK-418-SER5AAV-5 Helper Free Bicistronic Expression System (GFP) 1 kit
VPK-418-SER6AAV-6 Helper Free Bicistronic Expression System (GFP) 1 kit
VPK-419-DJAAV-DJ Helper Free Bicistronic Expression System (Blasticidin) 1 kit
VPK-419-DJ-8AAV-DJ/8 Helper Free Bicistronic Expression System (Blasticidin) 1 kit
VPK-419-SER1AAV-1 Helper Free Bicistronic Expression System (Blasticidin) 1 kit
VPK-419-SER2AAV-2 Helper Free Bicistronic Expression System (Blasticidin)1 kit
VPK-419-SER3AAV-3 Helper Free Bicistronic Expression System (Blasticidin) 1 kit
VPK-419-SER4AAV-4 Helper Free Bicistronic Expression System (Blasticidin) 1 kit
VPK-419-SER5AAV-5 Helper Free Bicistronic Expression System (Blasticidin) 1 kit
VPK-419-SER6AAV-6 Helper Free Bicistronic Expression System (Blasticidin) 1 kit