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 ǰȳ  ڻ   Viral Expression  AAV Expression

AAV Helper Free Packaging & Expression Systems

AAV Helper Free System live helper adenovirus ʿ, vector ̿Ͽ, gene AAV(adeno-associated virus) ϴ ýԴϴ.

Cell Biolabs
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  • Live helper virus ν ϰ gene delivery system Դϴ.
  • AAV packaging adenoviral genes pHelper plasmid (E2A, E4 and VA RNA) ǰų 293 packaging cells (E1)κ ޵˴ϴ.
  • 1-6 native serotypes AAV-DJ AAV-DJ/8 ޵˴ϴ.
  • AAV-DJ 8 ٸ native serotype (NAAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, caprine AAV) Ͽ hybrid capsid ν, پ tissue cell types native serotypes infection ȿ Դϴ.
  • AAV-DJ/8 AAV-DJ heparin binding domain point mutation Ų ǰ, ̸ recombinant AAV in vivo󿡼 heart, brain tissue native serotypes 8 (AAV-8), 9 (AAV-9) ϰ ˴ϴ.
  • Expression vector, GFP control vector, pHelper Vector Ǵ Packaging System expression vector Բ Ǵ Expression System ֽϴ.
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ComponentsPackaging SystemExpression System
pAAV Vector Yes Yes
pHelper Vector Yes Yes
pAAV-GFP Control Vector Yes Yes
pAAV Expression Vector - Yes
Figure 1: Viral Gene Delivery using the AAV Helper Free System.
Figure 1: Viral Gene Delivery using the AAV Helper Free System.
Figure 2:Production and Transduction of AAV2-GFP. Top Left: 293AAV cells prior to transfection (10X). Top Right: 293AAV cells 48 hours after transfection (10X). Bottom Left: GFP expression in 293AAV cells 48 hours after transfection (10X). Bottom Right: GFP expression in 293AD cells 48 hours after infection (20X).
Figure 2: Production and Transduction of AAV2-GFP. Top Left: 293AAV cells prior to transfection (10X). Top Right: 293AAV cells 48 hours after transfection (10X). Bottom Left: GFP expression in 293AAV cells 48 hours after transfection (10X). Bottom Right: GFP expression in 293AD cells 48 hours after infection (20X).
Figure 3: Generation of AAV-DJ through Capsid DNA Family Shuffling.
Figure 3: Generation of AAV-DJ through Capsid DNA Family Shuffling.

Relative in vitro Infectivity of AAV Vectors
Cell Line AAV-1 AAV-2 AAV-3 AAV-4 AAV-5 AAV-6 AAV-8 AAV-9 AAV-DJ AAV-DJ/8
Huh-7 13 100 2.5 0 0.1 10 0.7 0 500 0.2
HEK293 25 100 2.5 0.1 0.1 5 0.7 0.1 500 0.3
HeLa 3 100 2 0.1 6.7 1 0.2 0.1 667 0.2
HepG2 3 100 16.7 0.3 1.7 5 0.3 ND 1250 0.5
Hep1A 20 100 0.2 1 0.1 1 0.2 0 400 0.1
911 17 100 11 0.2 0.1 17 0.1 ND 500 0
CHO 100 100 14 1.4 333 50 10 1 25000 5
COS 33 100 33 3.3 5 14 2 0.5 500 0.3
MeWo 10 100 20 0.3 6.7 10 1 0.2 2857 1
NIH3T3 10 100