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 Á¦Ç°¾È³»  ºÐÀÚ»ý¹°ÇР  PCR Amplification  Direct PCR

Plant Direct PCR Kit

Plant·Î ºÎÅÍ DNA ÃßÃâ¾øÀÌ 10ºÐ¾È¿¡ ¹Ù·Î PCRÀ» ÁøÇàÇÒ ¼ö ÀÖ´Â kit ÀÔ´Ï´Ù.

KOMABIOTECH
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  • DNA¸¦ »ÌÁö¾Ê°í plant leafÀ¸·Î ¹Ù·Î PCRÀ» ¼öÇàÇϹǷÎ, ½Ã°£ÀÌ ´ÜÃàµÇ¸ç °£ÆíÇÕ´Ï´Ù.
  • Proteinase K¸¦ »ç¿ëÇÒ Çʿ䰡 ¾ø½À´Ï´Ù.
  • Harris Micro punch¸¦ ÀÌ¿ëÇÏ¿© Á¤±³ÇÏ°Ô 0.5mmÀÇ leaf¸¦ ¾òÀ» °æ¿ì¿¡´Â lysis °úÁ¤¾øÀÌ ¹Ù·Î PCR template·Î »ç¿ëÇÒ ¼ö ÀÖ½À´Ï´Ù.
  • Ä®µîÀ¸·Î Á¶°¢À» ³»¾î leaf¸¦ ¾òÀ» °æ¿ì¿¡´Â 85°C 10ºÐÀÇ lysis stepÀ» ó¸®ÈÄ, ÃßÃâ¾× 1ul¸¦ °®°í ¹Ù·Î PCRÀ» ÁøÇàÇÕ´Ï´Ù.
  • plant tissue¸¦ °¥°Å³ª, ¿©·¯ ¹öÆÛ ¹× DNA columnÀ» ÀÌ¿ëÇÏ´Â ÀüÅëÀûÀÎ ¹æ¹ý¿¡ ºñÇØ Á¶ÀÛÀÌ ¸Å¿ì °£´ÜÇÏ°í ½±½À´Ï´Ù.
  • Á¶ÀÛÀ» ÃÖ¼ÒÈ­ÇÔÀ¸·Î½á ¿À¿°ÀÇ À§Çè°ú ¹ø°Å·Î¿òÀ» ÁÙ¿©ÁÝ´Ï´Ù.
  • ½ÇÇè°úÁ¤ÀÌ ´Ü¼øÇÕ´Ï´Ù.
    1. 1) Plant leafÀ» ÀÚ¸¥´Ù.
         (Harris Micro punch·Î 0.5mm¸¦ ¾òÀ» ¼ö ÀÖ´Â °æ¿ì¿¡´Â 2,3¹ø °úÁ¤¾øÀÌ ¹Ù·Î PCR ¼öÇàÇÑ´Ù.)
    2. 2) ÇÔ²² Á¦°øµÇ´Â Lysis buffer¿¡ ³Ö°í 85°C¿¡¼­ 10ºÐ°£ incubation ÇÑ´Ù.
    3. 3) Vortex³ª spin down
    4. 4) »óÃþ¾× 1ul¸¦ ¶°¼­ ¹Ù·Î PCRÀ» ¼öÇàÇÑ´Ù.
Á¦Ç°ÀÇ ±¸¼º
2X Direct Taq PCR MasterMix   1ml
2.5X Plant Direct Lysis Buffer   1.5ml
4X Magic Buffer   1ml
Positive Control   20ul
Control Primer   20ul
(»ç¿ëȽ¼ö : 25ul reaction PCR½Ã 80¹ø »ç¿ë)
»ç¿ë¿¹
Direct PCR from Arabidopsis leaf
Direct PCR from Arabidopsis leaf:

PCR was performed with 1 ul Arabidopsis leaf lysate using the 1X Plant Lysis Buffer in 20 ul PCR. And, pieces of Arabidopsis leaf with 2 mm Harris Punch were placed directly into 20 ul PCR.
Lane 1, 6 and 11: negative control
Lane 2, 7 and 12: 1uL lysate
Lane 3, 8 and 13: one piece
Lane 4, 9 and 14: two pieces
Lane 5, 10 and 15: three pieces

 Direct PCR from Plant leaves
Direct PCR from Plant leaves:

PCR was performed with 1 ul Nicotiana and Capsisum leaf lysate using the 1X Plant Lysis Buffer in 20 ul PCR. And, pieces of Nicotiana and Capsisum leaf with 2 mm Harris Punch were placed directly into 20 ul PCR.
Lane 1 and 5: 1uL lysate
Lane 2 and 6: one piece
Lane 3 and 7: two pieces
Lane 4 and 8: three pieces
ÁÖ¹®Á¤º¸(Ordering Information)
CATNUM PRODUCT SIZE
K0568700EzWay Plant Direct PCR Kit1set