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 Á¦Ç°¾È³»  ºÐÀÚ»ý¹°ÇР  PCR Amplification  Direct PCR

EzWay¢â Mouse Tail Direct PCR Kit

Mouse tail·Î ºÎÅÍ DNA ÃßÃâ¾øÀÌ 10ºÐ¾È¿¡ ¹Ù·Î PCRÀ» ÁøÇàÇÒ ¼ö ÀÖ´Â kit ÀÔ´Ï´Ù.

KOMABIOTECH
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  • DNA¸¦ »ÌÁö¾Ê°í mouse tail·Î ¹Ù·Î PCRÀ» ¼öÇàÇϹǷÎ, ½Ã°£ÀÌ ´ÜÃàµÇ¸ç °£ÆíÇÕ´Ï´Ù.
  • Proteinase K (PK)¸¦ ó¸®ÇÒ Çʿ䰡 ¾ø¾î ºñ¿ë°ú ½ºÅÜÀ» ÁÙÀϼö ÀÖ½À´Ï´Ù.
  • Overnight incubationÀº ±×¸¸! ´Ü 10ºÐ lysis °úÁ¤À» ÅëÇØ template¸¦ ¾òÀ» ¼ö ÀÖ½À´Ï´Ù.
  • Hot Start TaqÀ» ÀÌ¿ëÇÏ¿© ºñƯÀÌ ¹ÝÀÀÀ» ÁÙÀ̰í PCR È¿À²À» ³ôÇû½À´Ï´Ù.
  • Direct PCR buffer¸¦ mouse tail Á¶°Ç¿¡ ÃÖÀûÈ­½ÃÄÑ, ÁÁÀº °á°ú¸¦ º¸ÀåÇÕ´Ï´Ù.
  • Á¶ÀÛÀ» ÃÖ¼ÒÈ­ÇÔÀ¸·Î½á ¿À¿°ÀÇ À§Çè°ú ¹ø°Å·Î¿òÀ» ÁÙ¿©ÁÝ´Ï´Ù.
  • Transgenic mouse genotyping¿¡ Àû¿ëÇϸé È¿°úÀûÀÔ´Ï´Ù.
  • Mouse ear·Îµµ »ç¿ëÀÌ °¡´ÉÇÕ´Ï´Ù.
  • ½ÇÇè°úÁ¤ÀÌ ´Ü¼øÇÕ´Ï´Ù.
    1. 1) Mouse tailÀ» 1-2mm Å©±â·Î ÀÚ¸¥´Ù.
    2. 2) ÇÔ²² Á¦°øµÇ´Â Lysis buffer¿¡ ³Ö°í 60°C¿¡¼­ 10ºÐ°£ incubation ÇÑ´Ù.
    3. 3) Vortex³ª spin down
    4. 4) »óÃþ¾× 1ul¸¦ ¶°¼­ ¹Ù·Î PCRÀ» ¼öÇàÇÑ´Ù.
Mouse Tail Genotyping 樨毢
Á¦Ç°ÀÇ ±¸¼º
2X EzWay Direct Taq PCR MasterMix   1ml
2.5X Mouse Tail Direct Lysis Buffer   1.5 ml X 3
4X Magic Buffer   1ml
Positive Control   20ul
Control Primer   20ul
(»ç¿ëȽ¼ö : 25ul reaction PCR½Ã 80¹ø »ç¿ë)
»ç¿ë¿¹
Direct PCR from mouse tail tissue :
  • 10mgÀÇ mouse tailÀ» lysis buffer·Î lysis¸¦ ¼öÇàÇÏ¿© °¢°¢ 2, 0.2, 0.04, 0.02ul ÀÇ ³óµµ·Î PCRÀ» ¼öÇàÇÏ¿´´Ù. PK 󸮾øÀÌ 60°C¿¡¼­ 10ºÐ incubation ¸¸À¸·Îµµ ±ú²ýÇÏ°Ô PCR ÁõÆøÀÌ ÀÌ·ç¾îÁüÀ» È®ÀÎÇÒ¼ö ÀÖ¾ú´Ù.
  • Lane 1,3,5,7 : Direct PCR after lysis using Proteinase K at 56°C for 10 min and 95°C for 5 min
  • Lane 2,4,6,8: Direct PCR without PK treatment. Incubation at 60°C for 10 min
ÁÖ¹®Á¤º¸(Ordering Information)
CATNUM PRODUCT SIZE
K0568600EzWay Mouse Tail Direct PCR Kit1set