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 Á¦Ç°¾È³»  Ç×ü,Ç׿ø,ŶƮ   Neuroscience  Oxidative Stress

Oxidative Protein Damage Kits

¼¼Æ÷³» ´Ü¹éÁúµéÀº ROS (Reactive Oxygen Species Ȱ¼º»ê¼Ò)¿Í reactive nitrogen species (RNS)¿¡ ÀÇÇØ ¼Õ»óÀ» ÀԱ⠽±½À´Ï´Ù. ´Ü¹éÁú ¼Õ»ó½Ã, ´Ü¹éÁúÀÇ amino±â¿¡ nitrationÀ̳ª oxidationÀÌ ÀϾ°Ô µÇ´Âµ¥, ÀÌ·¯ÇÑ Protein Oxidation / Nitration¿¡ °ü·ÃµÈ ¿©·¯ »ê¹°À» ÃøÁ¤ÇÏ´Â Á¦Ç°À» ¼Ò°³ÇÕ´Ï´Ù.

Cell Biolabs
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Oxidative Protein Damage Assay Selection Guide
Target¿ëµµ»ùÇà ŸÀÔCat. No.Ư¡
Protein carbonyl content (PCC) protein oxidationÀÇ ÀϹÝÀûÀÎ ¸¶Ä¿ÀÎ protein carbonyl content (PCC)¸¦ ÃøÁ¤ÇÏ´Â Á¦Ç°ÀÔ´Ï´Ù. Cell Lysate
Tissue
Serum/Plasma
STA-310 Protein carbonylÀ» ELISA·Î ÃøÁ¤ (10 μg/mL)
STA-315 Spectrophotometric ¹æ¹ýÀ¸·Î 1 mg/mL±îÁö ÃøÁ¤ °¡´É
STA-308 Immunoblot ¹æ¹ý
3-Nitrotyrosine »êÈ­Àû ½ºÆ®·¹½º(oxidative stress)ÀÇ ÁöÇ¥ ¹°ÁúÀÎ NitrotyrosineÀ» Á¤·®ÇÏ´Â Á¦Ç°ÀÔ´Ï´Ù. Cell Lysate
Tissue
Serum/Plasma
STA-305 NitrotyrosineÀ» ELISA·Î ÃøÁ¤ (10 nM)
STA-303 NitrotyrosineÀ» ImmunoblotÀ¸·Î ÃøÁ¤
17-376 NitrotyrosineÀ» Chemiluminescence ELISA·Î ÃøÁ¤
Advanced Glycation End Products (AGE) Advanced glycation end products (AGE)¿¡ ¼ÓÇÏ´Â adducts¸¦ ÃøÁ¤Çϱâ À§ÇÑ Á¦Ç°ÀÔ´Ï´Ù. Cell Lysate
Tissue
Serum/Plasma
STA-316 Nε-(carboxymethyl) lysine (CML) adducts¸¦ ELISA·Î ÃøÁ¤
STA-313 Nε-(carboxymethyl) lysine (CML) adducts¸¦ WBÀ¸·Î ÃøÁ¤
STA-317 AGE protein adducts¸¦ ELISA·Î ÃøÁ¤
STA-311 Methylglyoxal (MG) adducts¸¦ ELISA·Î ÃøÁ¤
STA-300 Nε-(carboxyethyl) lysine (CEL) adducts¸¦ ELISA·Î ÃøÁ¤
Advanced Oxidation Protein Products (AOPP) Advanced oxidation protein products (AOPP)ÀÇ Á¸À縦 ´Ü 30ºÐ¾È¿¡ ÃøÁ¤ÇØ ÁÖ´Â Á¦Ç°ÀÔ´Ï´Ù. Cell Lysate
Tissue
Plasma
STA-318 AOPPs ¾çÀ» Spectrophotometer·Î ÃøÁ¤ (5 μM)
BPDE Protein Adduct Benzo(a)pyrene diol epoxide (BPDE) protein adducts¸¦ Á¤·®Çϱâ À§ÇÑ Á¦Ç°ÀÔ´Ï´Ù. Cell Lysate
Tissue
Serum/Plasma
STA-301 BPDE-protein adduct¸¦ ELISA·Î ÃøÁ¤ (10 μg/mL)
Á¾·ù ¹× Ư¡
  • OxiSelect™ Protein Carbonyl Assays
    • protein carbonyl Çü¼ºÀ» Western blot ¹× ELISA, ȤÀº spectrophotometer ¹ýÀ¸·Î ÃøÁ¤ÀÌ °¡´ÉÇÕ´Ï´Ù.
    • plasma, serum, cell lysates, purified proteinsµîÀÇ »ùÇÃÀ» ÀÌ¿ëÇÒ ¼ö ÀÖ½À´Ï´Ù.
    • Immunoblot formatÀ» ÀÌ¿ë½Ã¿¡´Â, membrane»ó¿¡¼­ DNPH derivatizationÀ» ÅëÇØ oxidized & non-oxidized proteinÀ» ¹Ù·Î ºñ±³ÇÒ ¼ö ÀÖ½À´Ï´Ù.
    • ELISA formatÀ» ÀÌ¿ë½Ã¿¡´Â 10 ug/ml±îÁöµµ ¹Î°¨ÇÏ°Ô detection ÇÒ ¼ö ÀÖ½À´Ï´Ù.
    • ³óÃàÀ̳ª ħÀüµîÀÇ °úÁ¤À» °ÅÄ¡Áö ¾Ê¾Æ »ùÇà ¼Õ½Ç¿ì·Á°¡ Àû½À´Ï´Ù.
    • Á¾·ù:
      • STA-310 : OxiSelect™ Protein Carbonyl ELISA Kit
      • STA-315 : OxiSelect™ Protein Carbonyl Spectrophotometric Kit
      • STA-308 : OxiSelect™ Protein Carbonyl Immunoblot Kit
    Figure 1: Protein Carbonyl ELISA Standard Curve
    Figure 1: Protein Carbonyl ELISA Standard Curve
    Figure 2: Amount of Protein Carbonyls for Cell Lysate and BSA Standard. STO (MEF), HeLa and MDA-231 cells were sonicated in 25mM HEPES, pH 7.5, 150 mM NaCl, 10 mM MgCl2, 1 mM EDTA, 2% Glycerol. Cell Lysates and BSA Standard from Pierce BCA Protein Assay were diluted to 10 μg/mL with 1X PBS and coated onto a 96-well Protein Binding Plate. The protein carbonyl levels were determined as described in the Assay Protocol.
    Figure 2: Amount of Protein Carbonyls for Cell Lysate and BSA Standard. STO (MEF), HeLa and MDA-231 cells were sonicated in 25mM HEPES, pH 7.5, 150 mM NaCl, 10 mM MgCl2, 1 mM EDTA, 2% Glycerol. Cell Lysates and BSA Standard from Pierce BCA Protein Assay were diluted to 10 μg/mL with 1X PBS and coated onto a 96-well Protein Binding Plate. The protein carbonyl levels were determined as described in the Assay Protocol.
    Figure 3: Assay Principle of Protein Carbonyl Immunoblot Kit
    Figure 3: Assay Principle of Protein Carbonyl Immunoblot Kit
  • OxiSelect™ Nitrotyrosine ELISA Kit
    • Western Blot À̳ª ELISA ¹æ¹ýÀ¸·Î 3-Nitrotyrosine Çü¼ºÀ» ÃøÁ¤ÇÕ´Ï´Ù.
    • plasma, serum, cell lysates, purified proteinsµîÀÇ »ùÇÃÀ» ÀÌ¿ëÇÒ ¼ö ÀÖ½À´Ï´Ù.
    • Nitrated BSA°¡ positive control·Î Á¦°øµË´Ï´Ù.
    • Á¾·ù:
      • STA-305 : OxiSelect™ Nitrotyrosine ELISA Kit
      • STA-303 : OxiSelect™ Nitrotyrosine Immunoblot Kit
    Figure 1: Protein Nitration by tetranitromethane. STO (MEF) cells were lysed in 25mM HEPES, pH 7.5, 150 mM NaCl, 1% NP-40, 10 mM MgCl2, 1 mM EDTA, 2% Glycerol. Cell Lysate was nitrated with tetranitromethane (TNM). The protein 3-nitrotyrsone levels were determined as described in the assay instructions.
    Figure 1: Protein Nitration by tetranitromethane. STO (MEF) cells were lysed in 25mM HEPES, pH 7.5, 150 mM NaCl, 1% NP-40, 10 mM MgCl2, 1 mM EDTA, 2% Glycerol. Cell Lysate was nitrated with tetranitromethane (TNM). The protein 3-nitrotyrsone levels were determined as described in the assay instructions.
  • OxiSelect™ AOPP Assay Kit (STA-318)
    • cell lysates, tissue homogenates, plasmaµîÀÇ »ùÇÿ¡¼­ AOPP (Advanced Oxidation Protein Products)¸¦ ÃøÁ¤ÇÕ´Ï´Ù.
    • 30ºÐ¾È¿¡ ½Å¼ÓÈ÷ °á°ú¸¦ ¾òÀ»¼ö ÀÖ½À´Ï´Ù.
    • ÀÏ¹Ý microplate reader¸¦ ÀÌ¿ëÇϹǷΠÆí¸®ÇÕ´Ï´Ù.
    Figure 1. The AOPP-HSA Positive Control and untreated HSA were both prepared at a concentration of 100 μM and tested with STA-318 according to the assay protocol.
    Figure 1: The AOPP-HSA Positive Control and untreated HSA were both prepared at a concentration of 100 μM and tested with STA-318 according to the assay protocol.
Product Citations
STA-308
  • Jia, L. et al. (2007). Acrolein, a toxicant in cigarette smoke, causes oxidative damage and mitochondrial dysfunction in RPE cells: protection by (R)-alpha-lipoic acid. Invest. Ophthalmol. Vis. Sci. 48:339-348
  • Liu, Z. et al. (2007). Hydroxytyrosol protects retinal pigment epithelial cells from acrolein-induced oxidative stress and mitochondrial dysfunction. J. Neurochem. 103:2690-2700
  • Maity, P. et al. (2008). Indomethacin, a non-steroidal anti-inflammatory drug, develops gastropathy by inducing reactive oxygen species-mediated mitochondrial pathology and associated apoptosis in gastric mucosa: A novel role of mitochondrial aconitase oxidation. J. Biol. Chem. 284:3058-3068.
STA-310
  • Kim, S-H. et al. (2008). Protective effect of esculetin against oxidative stress-induced cell damage via scavenging reactive oxygen species. Acta Pharmacol. Sinica 29(11):1319-1326
  • Cho, H-Y. et al. (2009). Antiviral activity of Nrf2 in a murine model of respiratory syncytial virus (RSV) disease. Am. J. Respir. Crit. Care Med. 179:138-150
STA-305
  • Drel, V.R. et al. (2009). Poly(adenosine 5'-diphosphate-ribose) polymerase inhibition counteracts multiple manifestations of experimental type 1 diabetic nephropathy. Endocrinology 150:5273-5283
  • Cheah, F.-C. et al. (2008). Airway inflammatory cell responses to intra-amniotic lipopolysaccharide in a sheep model of chorioamnionitis. Am. J. Physiol. Lung Cell Mol. Physiol. 296:L384-L393
  • Li, X. et al. (2008). Lipoamide protects retinal pigment epithelial cells from oxidative stress and mitochondrial dysfunction. Free Radic. Biol. Med. 44(7):1465-1474