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 제품안내  면역학   Neuroscience  Oxidative Stress

Oxidative Protein Damage Kits

세포내 단백질들은 ROS (Reactive Oxygen Species 활성산소)와 reactive nitrogen species (RNS)에 의해 손상을 입기 쉽습니다. 단백질 손상시, 단백질의 amino기에 nitration이나 oxidation이 일어나게 되는데, 이러한 Protein Oxidation / Nitration에 관련된 여러 산물을 측정하는 제품을 소개합니다.
Cell Biolabs
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Oxidative Protein Damage Assay Selection Guide
Target용도샘플 타입Cat. No.특징
Protein carbonyl content (PCC) protein oxidation의 일반적인 마커인 protein carbonyl content (PCC)를 측정하는 제품입니다. Cell Lysate
Tissue
Serum/Plasma		 STA-310 Protein carbonyl을 ELISA로 측정 (10 μg/mL)
STA-315 Spectrophotometric 방법으로 1 mg/mL까지 측정 가능
STA-308 Immunoblot 방법
3-Nitrotyrosine 산화적 스트레스(oxidative stress)의 지표 물질인 Nitrotyrosine을 정량하는 제품입니다. Cell Lysate
Tissue
Serum/Plasma		 STA-305 Nitrotyrosine을 ELISA로 측정 (10 nM)
STA-303 Nitrotyrosine을 Immunoblot으로 측정
17-376 Nitrotyrosine을 Chemiluminescence ELISA로 측정
Advanced Glycation End Products (AGE) Advanced glycation end products (AGE)에 속하는 adducts를 측정하기 위한 제품입니다. Cell Lysate
Tissue
Serum/Plasma		 STA-316 Nε-(carboxymethyl) lysine (CML) adducts를 ELISA로 측정
STA-313 Nε-(carboxymethyl) lysine (CML) adducts를 WB으로 측정
STA-317 AGE protein adducts를 ELISA로 측정
STA-311 Methylglyoxal (MG) adducts를 ELISA로 측정
STA-300 Nε-(carboxyethyl) lysine (CEL) adducts를 ELISA로 측정
Advanced Oxidation Protein Products (AOPP) Advanced oxidation protein products (AOPP)의 존재를 단 30분안에 측정해 주는 제품입니다. Cell Lysate
Tissue
Plasma		 STA-318 AOPPs 양을 Spectrophotometer로 측정 (5 μM)
BPDE Protein Adduct Benzo(a)pyrene diol epoxide (BPDE) protein adducts를 정량하기 위한 제품입니다. Cell Lysate
Tissue
Serum/Plasma		 STA-301 BPDE-protein adduct를 ELISA로 측정 (10 μg/mL)
종류 및 특징
  • OxiSelect™ Protein Carbonyl Assays
    • protein carbonyl 형성을 Western blot 및 ELISA, 혹은 spectrophotometer 법으로 측정이 가능합니다.
    • plasma, serum, cell lysates, purified proteins등의 샘플을 이용할 수 있습니다.
    • Immunoblot format을 이용시에는, membrane상에서 DNPH derivatization을 통해 oxidized & non-oxidized protein을 바로 비교할 수 있습니다.
    • ELISA format을 이용시에는 10 ug/ml까지도 민감하게 detection 할 수 있습니다.
    • 농축이나 침전등의 과정을 거치지 않아 샘플 손실우려가 적습니다.
    • 종류:
      • STA-310 : OxiSelect™ Protein Carbonyl ELISA Kit
      • STA-315 : OxiSelect™ Protein Carbonyl Spectrophotometric Kit
      • STA-308 : OxiSelect™ Protein Carbonyl Immunoblot Kit
    Figure 1: Protein Carbonyl ELISA Standard Curve
    Figure 1: Protein Carbonyl ELISA Standard Curve
    Figure 2: Amount of Protein Carbonyls for Cell Lysate and BSA Standard. STO (MEF), HeLa and MDA-231 cells were sonicated in 25mM HEPES, pH 7.5, 150 mM NaCl, 10 mM MgCl2, 1 mM EDTA, 2% Glycerol. Cell Lysates and BSA Standard from Pierce BCA Protein Assay were diluted to 10 μg/mL with 1X PBS and coated onto a 96-well Protein Binding Plate. The protein carbonyl levels were determined as described in the Assay Protocol.
    Figure 2: Amount of Protein Carbonyls for Cell Lysate and BSA Standard. STO (MEF), HeLa and MDA-231 cells were sonicated in 25mM HEPES, pH 7.5, 150 mM NaCl, 10 mM MgCl2, 1 mM EDTA, 2% Glycerol. Cell Lysates and BSA Standard from Pierce BCA Protein Assay were diluted to 10 μg/mL with 1X PBS and coated onto a 96-well Protein Binding Plate. The protein carbonyl levels were determined as described in the Assay Protocol.
    Figure 3: Assay Principle of Protein Carbonyl Immunoblot Kit
    Figure 3: Assay Principle of Protein Carbonyl Immunoblot Kit
  • OxiSelect™ Nitrotyrosine ELISA Kit
    • Western Blot 이나 ELISA 방법으로 3-Nitrotyrosine 형성을 측정합니다.
    • plasma, serum, cell lysates, purified proteins등의 샘플을 이용할 수 있습니다.
    • Nitrated BSA가 positive control로 제공됩니다.
    • 종류:
      • STA-305 : OxiSelect™ Nitrotyrosine ELISA Kit
      • STA-303 : OxiSelect™ Nitrotyrosine Immunoblot Kit
    Figure 1: Protein Nitration by tetranitromethane. STO (MEF) cells were lysed in 25mM HEPES, pH 7.5, 150 mM NaCl, 1% NP-40, 10 mM MgCl2, 1 mM EDTA, 2% Glycerol. Cell Lysate was nitrated with tetranitromethane (TNM). The protein 3-nitrotyrsone levels were determined as described in the assay instructions.
    Figure 1: Protein Nitration by tetranitromethane. STO (MEF) cells were lysed in 25mM HEPES, pH 7.5, 150 mM NaCl, 1% NP-40, 10 mM MgCl2, 1 mM EDTA, 2% Glycerol. Cell Lysate was nitrated with tetranitromethane (TNM). The protein 3-nitrotyrsone levels were determined as described in the assay instructions.
  • OxiSelect™ AOPP Assay Kit (STA-318)
    • cell lysates, tissue homogenates, plasma등의 샘플에서 AOPP (Advanced Oxidation Protein Products)를 측정합니다.
    • 30분안에 신속히 결과를 얻을수 있습니다.
    • 일반 microplate reader를 이용하므로 편리합니다.
    Figure 1. The AOPP-HSA Positive Control and untreated HSA were both prepared at a concentration of 100 μM and tested with STA-318 according to the assay protocol.
    Figure 1: The AOPP-HSA Positive Control and untreated HSA were both prepared at a concentration of 100 μM and tested with STA-318 according to the assay protocol.
Product Citations
STA-308
  • Jia, L. et al. (2007). Acrolein, a toxicant in cigarette smoke, causes oxidative damage and mitochondrial dysfunction in RPE cells: protection by (R)-alpha-lipoic acid. Invest. Ophthalmol. Vis. Sci. 48:339-348
  • Liu, Z. et al. (2007). Hydroxytyrosol protects retinal pigment epithelial cells from acrolein-induced oxidative stress and mitochondrial dysfunction. J. Neurochem. 103:2690-2700
  • Maity, P. et al. (2008). Indomethacin, a non-steroidal anti-inflammatory drug, develops gastropathy by inducing reactive oxygen species-mediated mitochondrial pathology and associated apoptosis in gastric mucosa: A novel role of mitochondrial aconitase oxidation. J. Biol. Chem. 284:3058-3068.
STA-310
  • Kim, S-H. et al. (2008). Protective effect of esculetin against oxidative stress-induced cell damage via scavenging reactive oxygen species. Acta Pharmacol. Sinica 29(11):1319-1326
  • Cho, H-Y. et al. (2009). Antiviral activity of Nrf2 in a murine model of respiratory syncytial virus (RSV) disease. Am. J. Respir. Crit. Care Med. 179:138-150
STA-305
  • Drel, V.R. et al. (2009). Poly(adenosine 5'-diphosphate-ribose) polymerase inhibition counteracts multiple manifestations of experimental type 1 diabetic nephropathy. Endocrinology 150:5273-5283
  • Cheah, F.-C. et al. (2008). Airway inflammatory cell responses to intra-amniotic lipopolysaccharide in a sheep model of chorioamnionitis. Am. J. Physiol. Lung Cell Mol. Physiol. 296:L384-L393
  • Li, X. et al. (2008). Lipoamide protects retinal pigment epithelial cells from oxidative stress and mitochondrial dysfunction. Free Radic. Biol. Med. 44(7):1465-1474