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 제품안내  세포배양   Stem Cell  iPSC Generation

Vectors for iPSC Generation

iPSC Generation을 위한 Lentiviral vector 와 Retroviral vector를 소개합니다.
Cell Biolabs / GenTarget
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종류와 특징
    • Lentiviral Vectors for iPS Cell Generation (OCT3/4, SOX2, NANOG, LIN28, c-Myc, KLF4)
      • 6가지 Stem cell factor (OCT3/4, SOX2, NANOG, LIN28, c-Myc, KLF4)를 유도합니다.
      • Lentiviral particle을 이용하여 분열 및 미분열 세포 내로 gene을 transfer하는것이 용이합니다.
      • 안정적인 Lentiviral particles를 이용하여 long term expression이 가능합니다.
      • Human 과 mouse용 두가지를 가지고 있습니다.
      • 공통적으로 RFP-Blasticidine / Neomycine 마커를 포함하며, CMV promotor를 가지고 있습니다.
        공통적으로 RFP-Blasticidine / Neomycine 마커를 포함하며, CMV promotor를 가지고 있습니다.
      • Human의 경우, 그외에도 마커없이 EF1a promoter를 가지고 있는 타입으로 선택이 가능합니다.
        Human의 경우, 그외에도 마커없이 EF1a promoter를 가지고 있는 타입으로 선택이 가능합니다.
      • Inducible single vector particles를 이용하여 간단한 iPS cell generation이 가능합니다.
        Inducible single vector particles를 이용하여 간단한 iPS cell generation이 가능합니다.
    • Lentiviral Vectors for iPS Cell Generation (OCT3/4, SOX2, c-Myc, KLF4)
      • Klf4, Oct3/4, Sox2, c-Myc gene이 fusion된 Single polycistronic vector 입니다.
      • Lentivirus vector를 이용하여 분열및 미분열세포내로 gene을 transfer하는것이 용이합니다.
      • brain, liver, muscle, retina in vivo등에 독성이나 면역반응유발없이 잘 transfection 됩니다.
      • multi-virus transduction에 비해 10배 이상, non-viral methods에 비해 500배 이상 효율이 좋습니다.
      • GFP reporter를 포함하고 있습니다.
Schematic representation of pLentG-KOSM lentiviral vector (13 kb). Digestion with NotI and RsrII produces 3 kb and 10 kb DNA fragments.
    • Retroviral Vectors for iPS Cell Generation
      • Moloney murine leukemia virus (MMLV) 유래 pMXs retroviral vector를 이용하였습니다.
      • ampicillin-resistance gene, MMLV LTRs, package signal and human c-Myc gene cloned (NotI site)로 구성됩니다.
      • somatic cell로부터 iPS cells을 생성하기 위한 4-6가지 transcription factors를 발현합니다. (Oct3/4, Sox2, c-Myc, Klf4, NANOG and Lin-28),
      • Human, Mouse 용 vector가 제공됩니다.
Schematic representation of pMXs-hKlf4 retroviral vector (5.9 kb)
Schematic representation of pRetro-p53 shRNA (Human) retroviral vector
  • Retroviral Packaging Cells for iPS Cell Generation
    • 293T cell line 유래 potent retrovirus packaging cell line입니다.
    • Higher retroviral yields: average titer 10^7 infectious units/mL with transient transfection
    • Longer stability: up to 4 months in the presence of drug selection
    • Three versions: ecotropic (Plat-E), amphotropic (Plat-A), and pantropic (Plat-GP)
    • Citation : Li, W. et al. (2009). Rb/Cdk2/Cdk4 triple mutant mice elicit an alternative mechanism for regulation of the G1/S transition. PNAS 106:486-491. (#RV-101)
주문정보(Ordering Information)
CATNUM PRODUCT SIZE
LTV-451pLenti-p53 (Human) shRNA Lentiviral Vector100ul
LTV-700pLentG-KOSM Lentiviral Vector100ul
LVP-stems-h-EF1a-puroFull set (6 human stem factors expressed, EF1a-Puro)200ul x 6
LVP-stems-h-EF1a-RPFull set (6 human stem factors expressed, EF1a-RP)200ul x 6
LVP-stems-h-NFull set (6 human stem factors-with Neomycin marker)200ul x 6
LVP-stems-h-RBFull set (6 human stem factors-with RFP-Bsd marker)200ul x 6
LVP-stems-m-NFull set (6 mouse stem factors with Neomycin marker)200ul x 6
LVP-stems-m-RBFull set (6 mouse stem factors with RFP-Bsd marker)200ul x 6
LVP003 h OCT4 (RFP-Bsd) inducible particles1 x 108 IFU/ml x 200ul
LVP003mm OCT4 (RFP-Bsd) inducible particles 1 x 108 IFU/ml x 200ul
LVP004h SOX2 (RFP-Bsd) inducible particles1 x 108 IFU/ml x 200ul
LVP004mm Sox2 (RFP-Bsd) inducible particles 1 x 108 IFU/ml x 200ul
LVP005h NANOG (RFP-Bsd) inducible particles1 x 108 IFU/ml x 200ul
LVP005mm NANOG (RFP-Bsd) inducible particles 1 x 108 IFU/ml x 200ul
LVP006h LIN28 (RFP-Bsd) inducible particles1 x 108 IFU/ml x 200ul
LVP006mm LIN28 (RFP-Bsd) inducible particles 1 x 108 IFU/ml x 200ul
LVP007h cMyc (RFP-Bsd) inducible particles1 x 108 IFU/ml x 200ul
LVP007mm Myc (RFP-Bsd) inducible particles 1 x 108 IFU/ml x 200ul
LVP008h KLF4 (RFP-Bsd) inducible particles1 x 108 IFU/ml x 200ul
LVP008mm Klf4 (RFP-Bsd) inducible particles 1 x 108 IFU/ml x 200ul
LVP311 h OCT4 (Neo) inducible particles1 x 108 IFU/ml x 200ul
LVP311mm OCT4 (Neo) inducible particles 1 x 108 IFU/ml x 200ul
LVP312h SOX2 (Neo) inducible particles1 x 108 IFU/ml x 200ul
LVP312mm Sox2 (Neo) inducible particles 1 x 108 IFU/ml x 200ul
LVP313h NANOG (Neo) inducible particles1 x 108 IFU/ml x 200ul
LVP313mm NANOG (Neo) inducible particles 1 x 108 IFU/ml x 200ul
LVP314h LIN28 (Neo) inducible particles1 x 108 IFU/ml x 200ul
LVP314mm LIN28 (Neo) inducible particles 1 x 108 IFU/ml x 200ul
LVP315h Myc (Neo) inducible particles1 x 108 IFU/ml x 200ul
LVP315mm Myc (Neo) inducible particles 1 x 108 IFU/ml x 200ul
LVP316h KLF4 (Neo) inducible particles1 x 108 IFU/ml x 200ul
LVP316mm KLF4 (Neo) inducible particles1 x 108 IFU/ml x 200ul
LVP317 h OCT4 (EF1a) Lentiviral particles1 x 107 IFU/ml x 200ul
LVP318h SOX2 (EF1a) Lentiviral particles1 x 107 IFU/ml x 200ul
LVP319h NANOG (EF1a) Lentiviral particles1 x 107 IFU/ml x 200ul
LVP320h LIN28 (EF1a) Lentiviral particles1x107 IFU/ml x 200ul
LVP321h Myc (EF1a) Lentiviral particles1x107 IFU/ml x 200ul
LVP322h KLF4 (EF1a) Lentiviral particles1x107 IFU/ml x 200ul
LVP358-PuroChained human iPS (MKOS) (Puro) lentiviral particles1x106 IFU/ml x 0.5ml
LVP358-RPChained human iPS (MKOS) (RFP-Puro) lentiviral particle1x106 IFU/ml x 0.5ml
LVP359-PuroChained mouse iPS (MKOS) (Puro) lentiviral particles1x106 IFU/ml x 0.5ml
LVP359-RPChained mouse iPS (MKOS) (RFP-Puro) lentiviral particles1x106 IFU/ml x 0.5ml
LVP379Inducible chained human iPS (RFP-Bsd) 0.5 ml
LVP380Inducible chained human iPS (GFP-Bsd) 1 x 106 IFU/ml x 0.5ml
LVP381Inducible chained mouse iPS (RFP-Bsd) 1 x 106 IFU/ml x 0.5ml
LVP382Inducible chained mouse iPS (GFP-Bsd) 1 x 106 IFU/ml x 0.5ml
RTV-400pRetro-p53 shRNA Retroviral Vector100ul
RTV-410pRetro-p53 (Human) shRNA Retroviral Vector100ul
RTV-701pMXs-hOct3/4 Retroviral Vector10ug
RTV-701-CHuman Stem Cell Factor Retroviral Vector Set4 x 10ug
RTV-702pMXs-hSox2 Retroviral Vector10ug
RTV-703pMXs-hc-Myc Retroviral Vector10ug
RTV-704pMXs-hKlf4 Retroviral Vector10ug
RTV-705pMXs-mOct3/4 Retroviral Vector10ug
RTV-705-CMouse Stem Cell Factor Retroviral Vector Set4 x 10ug
RTV-706pMXs-mSox2 Retroviral Vector10ug
RTV-707pMXs-mc-Myc Retroviral Vector10ug
RTV-708pMXs-mKlf4 Retroviral Vector10ug
RTV-709pMXs-hNANOG Retroviral Vector10ug
RTV-709-CHuman Stem Cell Factor Retroviral Vector Set (6 Genes)6 x 10ug
RTV-710pMXs-hLin-28 Retroviral Vector10ug
RTV-711pMXs-mNANOG Retroviral Vector10ug
RTV-711-CMouse Stem Cell Factor Retroviral Vector Set (6 Genes)6 x 10ug
RTV-712pMXs-mLin-28 Retroviral Vector10ug
RV-101Platinum-E Retroviral Packaging Cell Line, Ecotrophic1 Vial
RV-102Platinum-A Retroviral Packaging Cell Line, Amphotrophic1 Vial
RV-103Platinum-GP Retroviral Packaging Cell Line, General Purpose1 Vial