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 Á¦Ç°¾È³»  ¼¼Æ÷¹è¾ç   Stem Cell  iPSC Generation

Vectors for iPSC Generation

iPSC GenerationÀ» À§ÇÑ Lentiviral vector ¿Í Retroviral vector¸¦ ¼Ò°³ÇÕ´Ï´Ù.

Cell Biolabs / GenTarget
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    • Lentiviral Vectors for iPS Cell Generation (OCT3/4, SOX2, NANOG, LIN28, c-Myc, KLF4)
      • 6°¡Áö Stem cell factor (OCT3/4, SOX2, NANOG, LIN28, c-Myc, KLF4)¸¦ À¯µµÇÕ´Ï´Ù.
      • Lentiviral particleÀ» ÀÌ¿ëÇÏ¿© ºÐ¿­ ¹× ¹ÌºÐ¿­ ¼¼Æ÷ ³»·Î geneÀ» transferÇϴ°ÍÀÌ ¿ëÀÌÇÕ´Ï´Ù.
      • ¾ÈÁ¤ÀûÀÎ Lentiviral particles¸¦ ÀÌ¿ëÇÏ¿© long term expressionÀÌ °¡´ÉÇÕ´Ï´Ù.
      • Human °ú mouse¿ë µÎ°¡Áö¸¦ °¡Áö°í ÀÖ½À´Ï´Ù.
      • °øÅëÀûÀ¸·Î RFP-Blasticidine / Neomycine ¸¶Ä¿¸¦ Æ÷ÇÔÇϸç, CMV promotor¸¦ °¡Áö°í ÀÖ½À´Ï´Ù.
        °øÅëÀûÀ¸·Î RFP-Blasticidine / Neomycine ¸¶Ä¿¸¦ Æ÷ÇÔÇϸç, CMV promotor¸¦ °¡Áö°í ÀÖ½À´Ï´Ù.
      • HumanÀÇ °æ¿ì, ±×¿Ü¿¡µµ ¸¶Ä¿¾øÀÌ EF1a promoter¸¦ °¡Áö°í Àִ ŸÀÔÀ¸·Î ¼±ÅÃÀÌ °¡´ÉÇÕ´Ï´Ù.
        HumanÀÇ °æ¿ì, ±×¿Ü¿¡µµ ¸¶Ä¿¾øÀÌ EF1a promoter¸¦ °¡Áö°í Àִ ŸÀÔÀ¸·Î ¼±ÅÃÀÌ °¡´ÉÇÕ´Ï´Ù.
      • Inducible single vector particles¸¦ ÀÌ¿ëÇÏ¿© °£´ÜÇÑ iPS cell generationÀÌ °¡´ÉÇÕ´Ï´Ù.
        Inducible single vector particles¸¦ ÀÌ¿ëÇÏ¿© °£´ÜÇÑ iPS cell generationÀÌ °¡´ÉÇÕ´Ï´Ù.
    • Lentiviral Vectors for iPS Cell Generation (OCT3/4, SOX2, c-Myc, KLF4)
      • Klf4, Oct3/4, Sox2, c-Myc geneÀÌ fusionµÈ Single polycistronic vector ÀÔ´Ï´Ù.
      • Lentivirus vector¸¦ ÀÌ¿ëÇÏ¿© ºÐ¿­¹× ¹ÌºÐ¿­¼¼Æ÷³»·Î geneÀ» transferÇϴ°ÍÀÌ ¿ëÀÌÇÕ´Ï´Ù.
      • brain, liver, muscle, retina in vivoµî¿¡ µ¶¼ºÀ̳ª ¸é¿ª¹ÝÀÀÀ¯¹ß¾øÀÌ Àß transfection µË´Ï´Ù.
      • multi-virus transduction¿¡ ºñÇØ 10¹è ÀÌ»ó, non-viral methods¿¡ ºñÇØ 500¹è ÀÌ»ó È¿À²ÀÌ ÁÁ½À´Ï´Ù.
      • GFP reporter¸¦ Æ÷ÇÔÇϰí ÀÖ½À´Ï´Ù.
Schematic representation of pLentG-KOSM lentiviral vector (13 kb). Digestion with NotI and RsrII produces 3 kb and 10 kb DNA fragments.
    • Retroviral Vectors for iPS Cell Generation
      • Moloney murine leukemia virus (MMLV) À¯·¡ pMXs retroviral vector¸¦ ÀÌ¿ëÇÏ¿´½À´Ï´Ù.
      • ampicillin-resistance gene, MMLV LTRs, package signal and human c-Myc gene cloned (NotI site)·Î ±¸¼ºµË´Ï´Ù.
      • somatic cell·ÎºÎÅÍ iPS cellsÀ» »ý¼ºÇϱâ À§ÇÑ 4-6°¡Áö transcription factors¸¦ ¹ßÇöÇÕ´Ï´Ù. (Oct3/4, Sox2, c-Myc, Klf4, NANOG and Lin-28),
      • Human, Mouse ¿ë vector°¡ Á¦°øµË´Ï´Ù.
Schematic representation of pMXs-hKlf4 retroviral vector (5.9 kb)
Schematic representation of pRetro-p53 shRNA (Human) retroviral vector
  • Retroviral Packaging Cells for iPS Cell Generation
    • 293T cell line À¯·¡ potent retrovirus packaging cell lineÀÔ´Ï´Ù.
    • Higher retroviral yields: average titer 10^7 infectious units/mL with transient transfection
    • Longer stability: up to 4 months in the presence of drug selection
    • Three versions: ecotropic (Plat-E), amphotropic (Plat-A), and pantropic (Plat-GP)
    • Citation : Li, W. et al. (2009). Rb/Cdk2/Cdk4 triple mutant mice elicit an alternative mechanism for regulation of the G1/S transition. PNAS 106:486-491. (#RV-101)
ÁÖ¹®Á¤º¸(Ordering Information)
CATNUM PRODUCT SIZE
LVP-stems-h-EF1a-puroFull set (6 human stem factors expressed, EF1a-Puro)200ul x 6
LVP-stems-h-EF1a-RPFull set (6 human stem factors expressed, EF1a-RP)200ul x 6
LVP-stems-h-NFull set (6 human stem factors-with Neomycin marker)200ul x 6
LVP-stems-h-RBFull set (6 human stem factors-with RFP-Bsd marker)200ul x 6
LVP-stems-m-NFull set (6 mouse stem factors with Neomycin marker)200ul x 6
LVP-stems-m-RBFull set (6 mouse stem factors with RFP-Bsd marker)200ul x 6
LVP003 h OCT4 (RFP-Bsd) inducible particles1 x 108 IFU/ml x 200ul
LVP003mm OCT4 (RFP-Bsd) inducible particles 1 x 108 IFU/ml x 200ul
LVP004h SOX2 (RFP-Bsd) inducible particles1 x 108 IFU/ml x 200ul
LVP004mm Sox2 (RFP-Bsd) inducible particles 1 x 108 IFU/ml x 200ul
LVP005h NANOG (RFP-Bsd) inducible particles1 x 108 IFU/ml x 200ul
LVP005mm NANOG (RFP-Bsd) inducible particles 1 x 108 IFU/ml x 200ul
LVP006h LIN28 (RFP-Bsd) inducible particles1 x 108 IFU/ml x 200ul
LVP006mm LIN28 (RFP-Bsd) inducible particles 1 x 108 IFU/ml x 200ul
LVP007h cMyc (RFP-Bsd) inducible particles1 x 108 IFU/ml x 200ul
LVP007mm Myc (RFP-Bsd) inducible particles 1 x 108 IFU/ml x 200ul
LVP008h KLF4 (RFP-Bsd) inducible particles1 x 108 IFU/ml x 200ul
LVP008mm Klf4 (RFP-Bsd) inducible particles 1 x 108 IFU/ml x 200ul
LVP311 h OCT4 (Neo) inducible particles1 x 108 IFU/ml x 200ul
LVP311mm OCT4 (Neo) inducible particles 1 x 108 IFU/ml x 200ul
LVP312h SOX2 (Neo) inducible particles1 x 108 IFU/ml x 200ul
LVP312mm Sox2 (Neo) inducible particles 1 x 108 IFU/ml x 200ul
LVP313h NANOG (Neo) inducible particles1 x 108 IFU/ml x 200ul
LVP313mm NANOG (Neo) inducible particles 1 x 108 IFU/ml x 200ul
LVP314h LIN28 (Neo) inducible particles1 x 108 IFU/ml x 200ul
LVP314mm LIN28 (Neo) inducible particles 1 x 108 IFU/ml x 200ul
LVP315h Myc (Neo) inducible particles1 x 108 IFU/ml x 200ul
LVP315mm Myc (Neo) inducible particles 1 x 108 IFU/ml x 200ul
LVP316h KLF4 (Neo) inducible particles1 x 108 IFU/ml x 200ul
LVP316mm KLF4 (Neo) inducible particles1 x 108 IFU/ml x 200ul
LVP317 h OCT4 (EF1a) Lentiviral particles1 x 107 IFU/ml x 200ul
LVP318h SOX2 (EF1a) Lentiviral particles1 x 107 IFU/ml x 200ul
LVP319h NANOG (EF1a) Lentiviral particles1 x 107 IFU/ml x 200ul
LVP320h LIN28 (EF1a) Lentiviral particles1x107 IFU/ml x 200ul
LVP321h Myc (EF1a) Lentiviral particles1x107 IFU/ml x 200ul
LVP322h KLF4 (EF1a) Lentiviral particles1x107 IFU/ml x 200ul
LVP358-PuroChained human iPS (MKOS) (Puro) lentiviral particles1x106 IFU/ml x 0.5ml
LVP358-RPChained human iPS (MKOS) (RFP-Puro) lentiviral particle1x106 IFU/ml x 0.5ml
LVP359-PuroChained mouse iPS (MKOS) (Puro) lentiviral particles1x106 IFU/ml x 0.5ml
LVP359-RPChained mouse iPS (MKOS) (RFP-Puro) lentiviral particles1x106 IFU/ml x 0.5ml
LVP379Inducible chained human iPS (RFP-Bsd) 0.5 ml
LVP380Inducible chained human iPS (GFP-Bsd) 1 x 106 IFU/ml x 0.5ml
LVP381Inducible chained mouse iPS (RFP-Bsd) 1 x 106 IFU/ml x 0.5ml
LVP382Inducible chained mouse iPS (GFP-Bsd) 1 x 106 IFU/ml x 0.5ml
RV-101Platinum-E Retroviral Packaging Cell Line, Ecotropic1 vial
RV-102Platinum-A Retroviral Packaging Cell Line, Amphotropic1 vial
RV-103Platinum-GP Retroviral Packaging Cell Line, Pantropic1 vial