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 제품안내  분자생물학   Genomic Research  DNA/RNA Purification & Quantitation

AccuBlue dsDNA Quantitation Kit

dsDNA정량에 방해가 될수 있는 오염원의 영향을 받지 않고 정확히 dsDNA를 정량할수 있는 kit로, PicoGreen™이나 Quant-iT™ dsDNA detection kits에 비해 안정하고 편리합니다.

Biotium
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특징
  • 다양한 농도의 정제된 dsDNA 샘플을 정확하게 정량하는 Kit 제품입니다.
  • 흡광도를 기반으로하는 nucleic acid 정량과는 달리 형광의 DNA binding dye가 double-stranded DNA에 매우 민감하게 결합하여 정확하게 DNA 농도를 측정합니다.
  • Free nucleotides, proteins, detergents, salts와 같은 오염물질과 RNA, ssDNA 등으로부터 영향이 거의 없습니다.
  • 각기 다른 농도 범위에 따라 3가지 제품이 제공되며, 이들 제품은 다양한 오염물질에 대한 내성에 차이가 있습니다.
종류
 AccuBlue™ Broad RangeAccuBlue™ High SensitivityAccuClear™ Ultra High Sensitivity
Cat. No. 31007 31006 31027
Linear range 2-2000 ng dsDNA 0.2-100 ng dsDNA 0.03-250 ng dsDNA
Fluorescence Blue (Ex/Em: 350/460 nm) Green (Ex/Em: 485/530 nm) Green (Ex/Em: 460/507 nm)
Detection fluorescence microplate reader fluorescence microplate reader fluorescence microplate reader
fluorospectrometer fluorospectrometer
fluorometer fluorometer
사용법
적용
Figure 1. Triplicate samples of calf thymus dsDNA, mouse liver RNA or salmon sperm ssDNA were assayed with the AccuBlue High Sensitivity dsDNA Quantitation Kit. The AccuBlue High Sensitivity Assay is selective for double-stranded DNA over RNA or single-stranded DNA.
AccuBlue™ Broad Range (2-200 ng)
Figure 2. Standard curve of calf thymus DNA assayed using AccuBlue Broad Range Kit and read on a microplate reader (Ex/Em 350/460). Inset shows the lower end of the titration.
AccuBlue™ Broad Range Vs. Quant-iT™ Broad Range (2-4000 ng)
Figure 3. Two-fold dilutions of calf thymus DNA were assayed using AccuBlue or Quant-iT Broad Range assay kits. AccuBlue has improved linearity and wider dynamic range than the Quant-iT Broad Range.
AccuBlue™ High Sensitivity (0.2-100 ng)
Figure 4. Standard curve of calf thymus DNA assayed using the AccuBlue High Sensitivity Kit and read on a microplate reader (Ex/Em 485/530). Inset shows the lower end of the titration.
AccuClear™ Ultra High Sensitivity (0.03-250 ng)
Figure 5. Standard curve of calf thymus DNA assayed using the AccuClear Ultra High Sensitivity Kit and read on a microplate reader (Ex/Em 460/507). Inset shows the lower end of the titration.
Figure 6. The AccuBlue High Sensitivity (HS) reagent and PicoGreen were diluted to working concentrations in HeLa cell cultures and incubated for 30 minutes. PicoGreen and the Quant-iT High Sensitivity reagent (Invitrogen) readily bind nuclear DNA while no nuclear staining is evident with the AccuBlue HS reagent, illustrating its overall safety and lower toxicity due to its membrane impermeability.