Actin polymerization Assay Kit
Actin binding protein은 세포의 형태와 운동성, 근육수축, intracellular trafficking, cell pathogenesis, signal transduction등의 연구에 관여됩니다. Actin polymerization kit은 actin의 polymerization kinetics를 연구하기위한 modified actin과 시약을 제공합니다.
- Actin polymerization kinetics를 연구하기 위한 modified actin과 시약을 제공합니다.
- Polymerization중에 발생하는 pyrene conjugated actin의 형광증가 정도를 측정해줍니다.
- non-muscle actin이나 cardiac actin등의 다른 타입의 polymerization 연구에도 사용될수 있습니다.
- 5 x 1 mg Pyrene labeled actin (Cat. # AP05).
- General Actin Buffer (Cat. # BSA01).
- Actin Polymerization Buffer (Cat. # BSA02).
- ATP 100mM (Cat. # BSA04).
- Tris-HCl pH 7.5, 100 mM
- Manual with detailed protocols and extensive troubleshooting guide.
- To show quantitative / qualitative effects on actin polymerization by the addition of a tissue extract, an actin binding protein or compound.
- To show quantitative / qualitative effects on actin polymerization by addition of an F-actin nucleating protein, compound or extract.
- To show quantitative / qualitative effects on steady-state F-actin levels by addition of an F-actin severing protein, compound or tissue extract.
- To show quantitative / qualitative effects on actin depolymerization by addition of an actin binding protein, compound or tissue extract.
The Actin Polymerization Biochem Kit™ was used to study the effects of Arp2/3 (Cat. # RP01) and the VCA domain of WASP (Cat. # VCG03) on actin polymerization rates. The Arp2/3 complex is an actin filament nucleator but has low nucleating/polymerizing activity on its own. The VCA domain of WASP is an activator of the Arp2/3 complex. Hence, when the Arp2/3 complex is mixed with the WASP VCA domain, these two exert a potent actin polymerizing activity (Fig. 1).
Figure 1. Actin polymerization stimulated by Arp2/3 complex and the VCA domain of WASP. Actin polymerization was measured using kit BK003. The addition of Arp2/3 complex or the VCA domain alone to actin has minimal effects on actin polymerization, while the combination of Arp2/3 and the VCA domain strongly stimulates the rate of actin polymerization.
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