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 제품안내  면역학   Cell Structure  Actin Assay

Actin binding protein spin-down Assay Kit, non-muscle

Actin binding protein은 세포의 형태와 운동성, 근육수축, intracellular trafficking, cell pathogenesis, signal transduction등의 연구에 관여됩니다. Actin binding kit은 고객께서 분리한 단백질의 Actin binding ability를 확인하는데 유용한 kit 입니다.

Cytoskeleton
제품 문의하기
인쇄하기

특징
  • F-actin 및 actin monomer에 binding하는 단백질을 확인하는데 사용됩니다.
  • Non-muscle tissue로부터 얻은 단백질의 actin filament에 대한 binding 능력을 결정하는데 필요한 시약과 control을 제공합니다.
Kit의 구성
  • 8 x 250 μg Non-muscle actin (Cat. # APHL99).
  • α-Actinin, positive control (Cat. # AT01).
  • BSA, negative control
  • General Actin Buffer (Cat. # BSA01).
  • Actin Polymerization Buffer (Cat.# BSA02)
  • F-actin Cushion Buffer.
  • ATP, 100 mM (Cat. # BSA04)
  • EGTA, 0.5 M
  • MgCl2, 100 mM
  • Tris-HCl pH 6.5, 1.0 M
  • Tris-HCl pH 7.5, 100 mM
  • Manual with detailed protocols and extensive troubleshooting guide
적용
  • To determine whether a protein binds to filaments or monomers of actin.
  • To determine whether a protein bundles F-actin
  • To test various conditions (e.g. pH optima) or requirements (e.g. divalent cation requirement) for binding to actin
실험논문
  • Falcon-Perez, J. M., Starcevic, M., Gautam, R. and Dell'Angelica, E. C. (2002). BLOC-1, a novel complex containing the pallidin and muted proteins involved in the biogenesis of melanosomes and platelet-dense granules. J. Biol. Chem. 277, 28191-28199.
  • Gohla, A., Birkenfeld, J. and Bokoch, G. M. (2005). Chronophin, a novel HAD-type serine protein phosphatase, regulates cofilin-dependent actin dynamics. Nat. Cell Biol. 7, 21-29.
  • Leung, C. L., Sun, D., Zheng, M., Knowles, D. R. and Liem, R. K. (1999). Microtubule actin cross-linking factor (MACF): a hybrid of dystonin and dystrophin that can interact with the actin and microtubule cytoskeletons. J. Cell Biol. 147, 1275-1286.
  • Zhai, L., Zhao, P., Panebra, A., Guerrerio, A. L. and Khurana, S. (2001). Tyrosine phosphorylation of villin regulates the organization of the actin cytoskeleton. J. Biol. Chem. 276, 36163-36167.
주문정보(Ordering Information)
CATNUM PRODUCT SIZE
BK013Actin Binding Protein Spin-Down Assay Kit (Non-muscle)30-100 assays