Actin binding protein spin-down Assay Kit, muscle
Actin binding protein은 세포의 형태와 운동성, 근육수축, intracellular trafficking, cell pathogenesis, signal transduction등의 연구에 관여됩니다. Actin binding kit은 고객께서 분리한 단백질의 Actin binding ability를 확인하는데 유용한 kit 입니다.
- F-actin 및 actin monomer에 binding하는 단백질을 확인하는데 사용됩니다.
- muscle tissue로부터 얻은 단백질의 actin filament에 대한 binding 능력을 결정하는데 필요한 시약과 control을 제공합니다.
- 8 x 250 μg Skeletal muscle actin (Cat. # AKL99).
- α-Actinin, positive control (Cat. # AT01).
- BSA, negative control
- General Actin Buffer (Cat. # BSA01).
- Actin Polymerization Buffer (Cat.# BSA02)
- F-actin Cushion Buffer.
- ATP, 100 mM (Cat. no. BSA04)
- EGTA, 0.5 M
- MgCl2, 100 mM
- Tris-HCl pH 6.5, 1.0 M
- Tris-HCl pH 7.5, 100 mM
- Manual with detailed protocols and extensive troubleshooting guide.
- To determine whether a protein binds to filaments or monomers of actin.
- To determine whether a protein bundles F-actin
- To test various conditions (e.g. pH optima) or requirements (e.g. divalent cation requirement) for binding to actin.
The actin binding protein spin-down assay kit was used to study the actin binding and bundling activities of α-actinin (cat. # AT01). α-Actinin was shown to bundle F-actin and make the F-actin sediment at 14,000 x g (Fig. 1).
Figure 1. Actin bundling assay using kit BK001. F-actin was incubated alone or together with α-actinin or BSA. Bundled F-actin was pelleted by a 14,000 x g centrifugation and pellets (P) and supernatants (S) were run on an SDS-PAGE gel. Only in the presence of the F-actin bundling protein α-actinin is actin pelleted at this centrifugation speed.
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