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 Á¦Ç°¾È³»  Ç×ü,Ç׿ø,ŶƮ   Neuroscience  Oxidative Stress

Lipid Peroxidation Assay Kit

Lipid peroxiationÀº µ¿½Ä¹°¿¡¼­, ³ëÈ­³ª Áúº´»óÅ¿¡¼­ ¹ß»ýÇÏ´Â ¼¼Æ÷¼Õ»ó(cellular damage) ¸ÞÄ¿´ÏÁòÁßÀÇ ÇϳªÀÔ´Ï´Ù. OxiSelect Lipid Peroxidation Assay´Â lipid peroxidation¿¡ ÀÇÇØ »ý°Ü³ª´Â ºÎ»ê¹° ¹× ¸¶Ä¿¸¦ ÃøÁ¤Çϱâ À§ÇÑ ¼Ö·ç¼ÇÀ» Á¦°øÇÕ´Ï´Ù.

Cell Biolabs
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Lipid Peroxidation Assay Selection Guide
Target¿ëµµ»ùÇà ŸÀÔCat. No.Ư¡
8-iso-Prostaglandin F2a (8-Isoprostane) iso-Prostaglandin F2a (8-iso-PGF2a)¸¦ ÃøÁ¤ÇÏ´Â Á¦Ç°ÀÔ´Ï´Ù. Cell Lysate
Tissue
Serum/Plasma
Urine
STA-337 8-iso-PGF2aÀÇ ¾çÀ» ELISA·Î 3½Ã°£³»¿¡ ÃøÁ¤
Malondialdehyde (MDA) Lipid peroxidationÀÇ »ê¹°ÀÎ malondialdehyde (MDA)¸¦ ÃøÁ¤ÇÏ´Â Á¦Ç°ÀÔ´Ï´Ù. Cell Lysate
Tissue
Serum/Plasma
Urine
STA-331 MDAÀÇ ¾çÀ» ImmunoblotÀ¸·Î ÃøÁ¤
STA-832 MDAÀÇ ¾çÀ» ELISA·Î ÃøÁ¤
TBARS Lipid peroxidationÀÇ »ê¹°ÀÎ total malondialdehyde (MDA)¸¦ TBA¿Í ¹ÝÀÀ½ÃÄÑ ÃøÁ¤ÇÏ´Â Á¦Ç°ÀÔ´Ï´Ù. Cell Lysate
Tissue
Serum/Plasma
Urine
STA-330 MDA-TBA adduct¸¦ ELISA³ª Çü±¤À¸·Î ÃøÁ¤. 30ºÐ¼Ò¿ä
4-Hydroynonenal (4-HNE) Lipid peroxidationÀÇ ºÎ»ê¹°ÀÎ HNE (4-hydroxynonenal)¸¦ anti-HNE·Î ÃøÁ¤ÇÏ´Â ¹æ¹ýÀÔ´Ï´Ù. Cell Lysate
Tissue
Serum/Plasma
STA-838 HNE adduct¸¦ ELISA·Î ÃøÁ¤


  • OxiSelect™ TBARS Assay Kit (MDA Quantitation) (STA-330)
    • Lipid peroxidationÀÇ »ê¹°ÀÎ total malondialdehyde (MDA)ÀÇ ¾çÀ», TBA¿Í ¹ÝÀÀ½ÃÄÑ MDA-TBA adduct¸¦ TBARS Method·Î ÃøÁ¤ÇÏ´Â kit ÀÔ´Ï´Ù.
    • oxidative stress¿©ºÎ¸¦ ´Ü 30ºÐ³»¿¡ ¾Ë¼ö ÀÖ½À´Ï´Ù.
    • ÀûÀº¾çÀÇ »ùÇ÷εµ ÃøÁ¤ÀÌ °¡´ÉÇÕ´Ï´Ù.
    • Ÿ»çÀÇ Á¦Ç°Àº TBARS ÃøÁ¤½Ã glass tubeµîÀÌ ÇÊ¿äÇÏÁö¸¸, OxiSelect´Â ÇÊ¿äÇÏÁö ¾Ê¾Æ »ç¿ëÀÌ Æí¸®ÇÕ´Ï´Ù.
    • Standard ELISA ¹ý ȤÀº fluorometric plate reader·Î ÃøÁ¤ÀÌ °¡´ÉÇÕ´Ï´Ù.
    • kit component
      1. MDA Standard (Part No. 233001): One amber vial of 1.0 mM Malondialdehyde bis(dimethylacetal) - 1.0 ml
      2. Thiobarbituric Acid (TBA) (Part No. 233002): One bottle - 1.0 gram
      3. SDS Lysis Solution (Part No. 233003): One bottle - 20 mL
      4. 2X TBA Acid Diluent (Part No. 233004): One bottle - 25 mL
      5. Sodium Hydroxide Solution (Part No. 233005): One bottle - 5 mL
    Figure 1: MDA Standard Curve. The MDA standard curve was created as described in the Assay Protocol. Top Panel: Colorimetric Detection; Bottom Panel: Fluorometric Detection.
    Figure 1: MDA Standard Curve. The MDA standard curve was created as described in the Assay Protocol. Top Panel: Colorimetric Detection; Bottom Panel: Fluorometric Detection.
  • OxiSelect™ MDA (Malondialdehyde) Assays (STA-331, STA-832)
    • Lipid peroxidationÀÇ »ê¹°ÀÎ malondialdehyde (MDA)¸¦ Immunoblot ȤÀº ELISA·Î ÃøÁ¤ÇÕ´Ï´Ù.
    • 1,2Â÷ Ç×ü ¹× positive controlÀ» Á¦°øÇÏ´Â OxiSelect MDA Immunoblot Kit¿Í ´õ ¸¹Àº ¾çÀÇ »ùÇÃÀ» µ¿½Ã¿¡ ÃøÁ¤ÇÒ¼ö ÀÖ´Â ELISA ¹ýÀ» ÀÌ¿ëÇÑ OxiSelect MDA ELISA kit¸¦ Á¦°øÇÕ´Ï´Ù.
    • ELISA Kit¸¦ ÀÌ¿ë½Ã 2 pmol/mgÀÇ ÀûÀº ³óµµ±îÁö ÃøÁ¤ÀÌ °¡´ÉÇÕ´Ï´Ù. 
    Figure 1: Immunoblotting with the OxiSelect™ MDA Immunoblot Kit (STA-331). MDA-BSA Immunoblot Control was electroblotted onto a nitrocellulose membrane. MDA was detected using an anti-MDA antibody as described in the assay protocol.
    Figure 1: Immunoblotting with the OxiSelect™ MDA Immunoblot Kit (STA-331). MDA-BSA Immunoblot Control was electroblotted onto a nitrocellulose membrane. MDA was detected using an anti-MDA antibody as described in the assay protocol.
     


    • OxiSelect™ 8-iso-Prostaglandin F2a ELISA Kit (8-isoprostane) (STA-337)
    • 8-iso-Prostaglandin F2a (8-iso-PGF2a)´Â membrane phospholipid¿¡¼­ »ý¼ºµÇ¸ç, atherogenesis, rhematoid arthritis, carcinogenesis°°Àº Áúº´°ú °ü·ÃµÇ¾î ÀÖ½À´Ï´Ù.
    • »ùÇó» 8-iso-PGF2aÀÇ ¾çÀ» 3½Ã°£³»¿¡ ½Å¼ÓÈ÷ ÃøÁ¤ÇØ ÁÝ´Ï´Ù.
    • 8-iso-PGF2a¸¦ positive control·Î Á¦°øÇÕ´Ï´Ù.
    • urine, plasma, serum, cell lysateµîÀÇ ´Ù¾çÇÑ »ùÇÿ¡¼­ ÃøÁ¤°¡´ÉÇÕ´Ï´Ù.
    • kit component
      1. 1. Goat Anti-Rabbit Antibody Coated Plate (Part No. 250001): One 96-well strip plate.
      2. 2. Anti-8-iso-PGF2α Antibody (Part No. 233701): One 20 μL tube of anti-8-iso-PGF2α rabbit IgG.
      3. 3. Sample Diluent (Part No. 233702): One 50 mL bottle.
      4. 4. 10X Wash Buffer (Part No. 310806): One 100 mL bottle.
      5. 5. Substrate Solution (Part No. 310807): One 12 mL amber bottle.
      6. 6. Stop Solution (Part. No. 310808): One 12 mL bottle.
      7. 7. 8-iso-PGF2α Standard (Part No. 233703): One 25 μL tube of 200 μL/mL 8-iso-PGF2α in DMSO.
      8. 8. 8-iso-PGF2α-HRP Conjugate (Part No. 233704): One 70 μL tube of 8-iso-PGF2α-HRP conjugate.
Figure 1: Dilutions of Human Urine Tested with the OxiSelect™ 8-iso-Prostaglandin F2alpha ELISA Kit (STA-337).
Figure 1: Dilutions of Human Urine Tested with the OxiSelect™ 8-iso-Prostaglandin F2alpha ELISA Kit (STA-337).


 
 Product Citations
STA-308
  • Mukhopadhyay, P. et al. (2009). CB1 cannabinoid receptors promote oxidative stress and cell death in murine models of doxorubicin-induced cardiomyopathy in human cardiomyocytes. Cardiovasc. Res. 10.1093/cvr/cvp369.
  • Singh, R. et al. (2009). Chronic oxidative stress sensitizes hepatocytes to death from 4-hydroxynonenal by JNK/c-Jun overactivation. Am.J. Physiol. Gastrointest. Liver Physiol. 10.1152/ajpgi.00151.2009.
  • Resende R. et al. (2008). Brain oxidative stress in a triple-transgenic mouse model of Alzheimer disease. Free Radic. Biol. Med. 44(12):2051-7.
STA-330
  • Song, Y.R. et al. (2010). Activation of hypoxia-inducible factor attenuates renal injury in rat remnant kidney. Nephrol. Dial. Transplant. 25:77-85.
  • Fomenko, D.E. et al. (2008). Methionine-R-sulfoxide reductase 1 (MsrB1) knockout mice: Roles of MsrB1 in redox regulation and identification of a novel selenoprotein form. J. Biol. Chem. 284:5986-5993.
  • Fujita, K. et al. (2008). Effectiveness of antiplatelet drugs against experimental non-alcoholic fatty liver disease. Gut 57:1583-1591.
STA-331
  • Barabutis, N. et al. (2008). Antioxidant activity of growth hormone-releasing hormone antagonists in LNCaP human prostate cancer line. PNAS 105:20470-20745 (#STA-331).