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 제품안내  면역학   Stem Cell  Stem Cell Assay

Alkaline Phosphatase Kit

Alkaline Phosphatase (AP)를 이용하여 ES 및 EG 세포의 분화와 미분화를 모니터링하기위한 kit 입니다.

Cell Biolabs / BioAssay System
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특징
  • ES(embryonic stem) cell과 EG(embryonic germ) cell의 marker로 널리 이용되는 Alkaline Phosphatase (AP)를 통해 세포의 분화, 미분화를 모니터링 합니다.
  • immunocytochemistry staining 및 quantitative colorimetric assay 두가지 방법으로 AP activity를 측정할수 있습니다.
  • 샘플의 species나 stem cell 여부와 상관없이 Alkaline phosphatase를 expression 하는 cell에는 모두 사용할수 있습니다.
종류
  • StemTAG™ Alkaline Phosphatase Staining Kit (CBA-300)
    • ICC staining방법으로 ES cell의 분화, 미분화를 측정할수 있는 제품입니다.
    • 제품의 구성 :
      • Fixing Solution (Part No. C30001): One bottle - 50 mL
      • StemTAG™ AP Staining Solution A (Part No. C30002): One amber bottle - 20 mL
      • StemTAG™ AP Staining Solution B (Part No. C30003): One amber bottle - 20 mL
    Figure 1: AP staining of ES Cells. Murine embryonic stem cells (ES-D3) are maintained in an undifferentiated stage on gelatin-coated dishes in the presence of LIF, as indicated by the high AP activity. To induce differentiation, LIF was withdrawn over a period of several days; various differentiation events were observed (cells became flattened and enlarged with reduced proliferation). At the end of day 5, AP staining of differentiated cells was performed as described in the Assay Protocol.
    Figure 1: AP staining of ES Cells. Murine embryonic stem cells (ES-D3) are maintained in an undifferentiated stage on gelatin-coated dishes in the presence of LIF, as indicated by the high AP activity. To induce differentiation, LIF was withdrawn over a period of several days; various differentiation events were observed (cells became flattened and enlarged with reduced proliferation). At the end of day 5, AP staining of differentiated cells was performed as described in the Assay Protocol.
  • StemTAG™ Alkaline Phosphatase Activity Assay Kit (CBA-301)
    • 정량분석에 의해 ES cell의 분화, 미분화를 측정할수 있는 제품입니다.
    • 제품의 구성 :
      • StemTAG™ AP Activity Assay Substrate (Part No. C30004): One bottle - 5 mL
      • Cell Lysis Buffer (Part No. C30005): One bottle - 20 mL
      • 10 x Stop Solution (Part No. C30006): One bottle - 10 mL
      • AP Activity Assay Standard (Part No. C30007): One tube - 1 mL of 5 mM p-Nitrophenol
    Figure 1: pNP Standard Curve and AP Activity Assay. A: A serial 2-fold dilution of pNP standard was prepared in 1X Stop Solution, and the absorbance of each dilution was measured at 405 nm. B: Mouse embryonic D3 cells were grown in the presence or absence of LIF for 5 days. 10 μg of cell lysate was assayed for AP activity according to the Activity Assay Instructions.
    Figure 1: pNP Standard Curve and AP Activity Assay. A: A serial 2-fold dilution of pNP standard was prepared in 1X Stop Solution, and the absorbance of each dilution was measured at 405 nm. B: Mouse embryonic D3 cells were grown in the presence or absence of LIF for 5 days. 10 μg of cell lysate was assayed for AP activity according to the Activity Assay Instructions.
  • StemTAG™ Alkaline Phosphatase Staining and Activity Assay Kit (CBA-302)
    • ICC staining 및 정량분석방법으로 ES cell의 분화, 미분화를 측정할수 있는 제품입니다.
    • 즉, 이용의 편리를 위해, CBA-300과 CBA-301을 각 100회 분량으로 같이 제공하는 제품입니다.
    • 제품의 구성 :
      • Fixing Solution (Part No. C30001): One bottle - 50 mL
      • StemTAG™ AP Staining Solution A (Part No. C30002): One amber bottle - 20 mL
      • StemTAG™ AP Staining Solution B (Part No. C30003): One amber bottle - 20 mL
      • StemTAG™ AP Activity Assay Substrate (Part No. C30004): One bottle - 5 mL
      • Cell Lysis Buffer (Part No. C30005): One bottle - 20 mL
      • 10 x Stop Solution (Part No. C30006): One bottle - 10 mL
      • AP Activity Assay Standard (Part No. C30007): One tube - 1 mL of 5 mM p-Nitrophenol
  • QuantiChrom™ Alkaline Phosphatase Assay Kit
    • Clorimetric 방법을 이용하여 샘플내 Alkaline Phosphatase의 activity를 측정하는 kit입니다.
    • 제품의 구성 :
      • Assay Buffer: 50 mL, pH 10.5
      • Mg Acetate: 1.5 mL 0.2 M
      • pNPP Liquid: 600 ul 1 M
      • Calibrator: 10 mL Tartrazine
 
Product Citations
CBA-300
  • Izadyar, F. et al. (2008). Generation of multipotent cell lines from a distinct population of male germ line stem cells. Reproduction 135:771-784.
  • Dharmacon Application Note: DharmaFect Transfection Reagents for siRNA Transfection into Embryonic Stem Cells (2006). Publication # 00078-06-E-01-U.
CBA-301
  • Ghosh, A. et al. (2007). Efficient whole-body transduction with trans-splicing adeno-associated viral vectors. Mol. Ther. 15(4):750-755.
  • Shi, X. et al. (2007). The oncogenic potential of a prostate cancer-derived androgen receptor mutant. The Prostate 67(6):591-602.
  • Yue, Y. et al. (2008). A single intravenous injection of adeno-associated virus serotype-9 leads to whole body skeletal muscle transduction in dogs. Mol. Ther. 16(12):1944-1952.
  • Kim, Y. et al. (2009). Cyclin-dependent kinase 2-associating protein 1 commits murine embryonic stem cell differentiation through retinoblastoma protein regulation. J. Biol. Chem. 284:23405-23414.