• Home
  • About us
  • Log-In
  • Join Us
  • Sitemap
  • Recruit
Á¦Ç°/½ÇÇè Q&A
ÀÚÁÖ¹¯´ÂÁú¹®
ÀÚÀ¯°Ô½ÃÆÇ
½ÇÇè±â¹ý
ÀÚ·á½Åû
³í¹®ÀÚ·á
Ŭ·¹ÀÓ Á¢¼ö
±â±â A/S Á¢¼ö
Ŭ·¹ÀÓ ÁøÇàÁ¶È¸
 Á¦Ç°¾È³»  Ç×ü,Ç׿ø,ŶƮ   Cell Analysis  Cell Transformation

CytoSelect¢â Clonogenic Tumor Cell Isolation Kit

CytoSelect¢â Clonogenic Tumor Cell Isolation Kit´Â heterogeneousÇÑ °íÇü ¾Ï ½Ã·á·ÎºÎÅÍ Á¤»ó¼¼Æ÷´Â Á¦°ÅÇÏ°í ¾Ï¼¼Æ÷¸¸À» ºÐ¸®ÇØ ÁÖ´Â kitÀÔ´Ï´Ù.

°íÇü¾Ï biopsy »ùÇÃÀ» lysisÇÏ¿©, semisolid agar media¿¡¼­ 6~8Àϰ£ ¹è¾çÇÏ¸é µË´Ï´Ù.

Cell Biolabs
Á¦Ç° ¹®ÀÇÇϱâ
ÀμâÇϱâ

Ư¡
  • Tumor cellÀ» ½±°í, È¿À²ÀûÀ¸·Î ºÐ¸®ÇÒ ¼ö ÀÖ½À´Ï´Ù.
  • ºÐ¸®µÈ tumor cellÀº FACS, protein/DNA array analysis, cancer vaccine development ºÐ¼®¿¡ »ç¿ëµË´Ï´Ù.
  • ÇϳªÀÇ Å°Æ®¿¡´Â 6-well plate³ª 35 mm dish¿¡¼­ 5¹ø ºÐ¸®Çϴµ¥ ÇÊ¿äÇÑ ½Ã¾àÀÌ Á¦°øµË´Ï´Ù.
  • ¸ðµç ±¸¼ºÇ°Àº 4¡É¿¡ º¸°üÇÕ´Ï´Ù.
»ç¾ç
  • Á¦Ç°±¸¼º
    • 10 x CytoSelect™ Agar Matrix Solution (Part No. 114001): One sterile tube - 1.8 mL
    • CytoSelect™ Matrix Diluent (Part No. 114002): One sterile bottle - 4.0 mL
    • 5 x DMEM Medium (Part No. 20103): One sterile bottle - 5.0 mL
    • 100 x Osmotic Lysis Buffer (Part No. 115501): One sterile tube - 1.0 mL
    • 10 x Assay Buffer (Part No. 115502): One sterile bottle - 15.0 mL
    • Filters (Part No. 115503): Five sterile filters
°úÁ¤
Àû¿ë
Figure 1. Clonogenic Colony Formation, Isolation, and Re-plating. Mouse lung tumor was excised, minced and digested before seeding 500,000 cells/well in agar matrix suspension, according to the assay protocol. Panel A demonstrates clonogenic colony formation after 7 days (red arrows show colonies, black arrows show single cells). Panel B demonstrates recovered clonogenic colonies (single cells have been removed). Panel C demonstrates re-plated clonogenic colonies after 3 days (without prior typsinization). Panel D demonstrates re-plated clonogenic colonies after 1 day (colonies weretypsinized/titurated to create a single-celled suspension before plating).