CytoSelect™ Clonogenic Tumor Cell Isolation Kit
|
|
|
|
|
CytoSelect™ Clonogenic Tumor Cell Isolation Kit는 heterogeneous한 고형 암 시료로부터 정상세포는 제거하고 암세포만을 분리해 주는 kit입니다.
고형암 biopsy 샘플을 lysis하여, semisolid agar media에서 6~8일간 배양하면 됩니다. |
|
| Cell Biolabs |
- Tumor cell을 쉽고, 효율적으로 분리할 수 있습니다.
- 분리된 tumor cell은 FACS, protein/DNA array analysis, cancer vaccine development 분석에 사용됩니다.
- 하나의 키트에는 6-well plate나 35 mm dish에서 5번 분리하는데 필요한 시약이 제공됩니다.
- 모든 구성품은 4℃에 보관합니다.
- 제품구성
- 10 x CytoSelect™ Agar Matrix Solution (Part No. 114001): One sterile tube - 1.8 mL
- CytoSelect™ Matrix Diluent (Part No. 114002): One sterile bottle - 4.0 mL
- 5 x DMEM Medium (Part No. 20103): One sterile bottle - 5.0 mL
- 100 x Osmotic Lysis Buffer (Part No. 115501): One sterile tube - 1.0 mL
- 10 x Assay Buffer (Part No. 115502): One sterile bottle - 15.0 mL
- Filters (Part No. 115503): Five sterile filters
과정
Figure 1. Clonogenic Colony Formation, Isolation, and Re-plating. Mouse lung tumor was excised, minced and digested before seeding 500,000 cells/well in agar matrix suspension, according to the assay protocol. Panel A demonstrates clonogenic colony formation after 7 days (red arrows show colonies, black arrows show single cells). Panel B demonstrates recovered clonogenic colonies (single cells have been removed). Panel C demonstrates re-plated clonogenic colonies after 3 days (without prior typsinization). Panel D demonstrates re-plated clonogenic colonies after 1 day (colonies weretypsinized/titurated to create a single-celled suspension before plating).
제품안내 
항체,항원,킷트 
Webmaster for more information.
