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CF Dyes´Â fluorescent antibody dye·Î, ±âÁ¸ÀÇ antibody labeling dye°¡ photostability, specificity, labeling efficient, chemical stability¸é¿¡ ÀÖ¾î ºÎÁ·Çß´ø ¸éÀ» Çâ»ó½ÃÄÑ, ÈξÀ °Çϰí, ºû°ú ÁÖÀ§È¯°æ¿¡ ¾ÈÁ¤Çϸç in vivo¿¡¼µµ Àå±â°£ °üÂûµÉ¼ö ÀÖ½À´Ï´Ù. |
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Biotium |
CF™ dye | λex (nm) |
λem (nm) |
Replacement for | ÀåÁ¡ |
CF™350 | 347 | 448 | Alexa Fluor® 350, AMCA, DyLight™ 350 |
• 350nm excitation½Ã °¡Àå ¹àÀº blue Çü±¤ • ¹°¿¡ ¸Å¿ì Àß ³ìÀ¸¸ç, pH insensitive |
CF™405S | 404 | 431 | Alexa Fluor® 405, Cascade Blue®, DyLight™ 405 |
• ÀϹÝÀûÀÎ ±â±âµé°ú ȣȯ¼º ÁÁÀ½ |
CF™405M | 408 | 452 | BD Horizon™ V450, eFluor® 450, Pacific Blue® |
• spill-over Çö»óÀÌ Àû¾î photostable • SIM super-resolution imaging¿¡ ÃÖÀû |
CF™405L | 395 | 545 | Pacific Orange® | • multicolor detectionÀ» À§ÇÑ orange Ä÷¯ |
CF™430 | 426 | 498 | Pacific Green®, BD Horizon™ V500, Krome Orange |
• photostable 405nm excitable green dye |
CF™450 | 450 | 538 | Unique dye | • µ¶Ã¢ÀûÀÎ ÆÄÀ寝¼ºÀ» °®´Â green dye |
CF™488A | 490 | 515 | ATTO 488, Alexa Fluor® 488, Cy®2, DyLight® 488, FAM, FITC, Fluorescein |
• ºñƯÀÌ ¹ÝÀÀÀ̳ª red spilloverÀÌ ÀûÀ½ • Çü±¤ÀÌ ¸Å¿ì ¾ÈÁ¤ • TIRF super resolution imaging¿¡ ȣȯµÊ |
CF™514 | 516 | 548 | Alexa Fluor® 514 | • spectral unmixingÀ¸·Î CF 488A¿Í ±¸ºÐµÇ´Â green dye |
CF™532 | 527 | 558 | Alexa Fluor® 532, ATTO 532 |
• Alexa Fluor® 532¿¡ ºñÇØ Çü±¤ÀÌ ÈξÀ ¹àÀ½ |
CF™535ST | 535 | 568 | Unique dye for STORM | • STORM super-resolution microscopy Àü¿ë orange dye |
CF™543 | 541 | 560 | Alexa Fluor® 546, Tetramethylrhodamine (TAMRA) | • Alexa Fluor® 546¿¡ ºñÇØ Çü±¤ÀÌ ÈξÀ ¹àÀ½ |
CF™555 | 555 | 565 | Alexa Fluor® 555, ATTO™550, Cy™3, DyLight™549, TRITC |
• Cy™3º¸´Ù ´õ ¹àÀ¸¸ç STORM multicolor super-resolution imaging¿¡ validationµÊ |
CF™568 | 562 | 583 | Alexa Fluor® 568, Rhodamine Red, ATTO™565 |
• Ar-Kr mixed-gas laser 568nm¿¡ ÃÖÀûÈ • Alexa Fluor® 568¿¡ ºñÇØ Çü±¤ÀÌ ÈξÀ ¹àÀ½ • TIRF, multicolor STORM¿¡ ȣȯ |
CF™594 | 593 | 614 | Alexa Fluor® 594, ATTO™594 DyLight™ 594 Texas Red® |
• À¯»ç ÆÄÀåÀÇ dyeÁß °¡Àå ¹àÀ½ • Çü±¤ÀÌ ¸Å¿ì ¾ÈÁ¤ÇÔ |
CF™594ST | 593 | 614 | Unique dye for STORM | • STORM super-resolution microscopy Àü¿ë dye |
CF™620R | 617 | 639 | LightCycler® Red 640 (LC640) | • µ¶Ã¢ÀûÀÎ ÆÄÀ寝¼ºÀ» °®´Â dye |
CF™633 | 630 | 650 | Alexa Fluor® 633, Alexa Fluor® 647, Cy™5, DyLight™ 633 |
• À¯»ç ÆÄÀåÀÇ antibody conjugates dyeÁß °¡Àå ¹àÀ½ • Alexa Fluor® 647º¸´Ù Çü±¤ÀÌ ¸Å¿ì ¾ÈÁ¤ÇÔ • TIRF, FIONA, gSHRImP¿¡ ȣȯ |
CF™640R | 642 | 662 | Alexa Fluor® 647, ATTO 647N, Cy®5, DyLight® 649 |
• Cy®5-like spectraÁß °¡Àå ÁÁÀº photostability À¯Áö • Yields highly fluorescent protein conjugates • TIRF, FLIMP super-resolution techniques°ú ȣȯ |
CF™647 | 650 | 665 | Alexa Fluor® 647, ATTO 647N, Cy®5, DyLight® 649 |
• Cy®5 º¸´Ù ¹àÀ½ • STORM multicolor super-resolution imaging¿¡ ȣȯ |
CF™660C | 667 | 685 | Alexa Fluor® 660 | • Alexa Fluor® 660º¸´Ù ´õ ¹à°í ¾ÈÁ¤ÇÔ • STORM multicolor super-resolution imaging¿¡ ȣȯ |
CF™660R | 663 | 682 | Alexa Fluor® 660 | • Alexa Fluor® 660º¸´Ù ´õ ¹àÀ½ • 660nm dye Áß °¡Àå photostable |
CF™680 | 681 | 698 | Alexa Fluor® 680, Cy®5.5, DyLight® 680, IRDye® 680LT |
• 680nm dye Áß °¡Àå ¹àÀ½ • multicolor STORM, dual-color 3D super-resolution imaging¿¡ validation µÊ • LI-COR® Odyssey® System°ú ȣȯ |
CF™680R | 680 | 701 | Alexa Fluor® 680, Cy®5.5, DyLight® 680, IRDye® 680LT |
• 680nm dye Áß °¡Àå photostable • small biomolecule°ú nucleic acid ¶óº§¸µ¿¡ ÀûÇÕ • LI-COR® Odyssey® System°ú ȣȯ • STED and single molecule spectroscopy¿Í ȣȯ |
CF™750 | 757 | 777 | Alexa Fluor® 750, Cy®7, DyLight® 750, IRDye® 750 |
• ¹à±â¿Í ¾ÈÁ¤¼ºÀÌ ¸Å¿ì ¶Ù¾î³² • Highly water soluble without bearing excessive charge • STORM super-resolution imaging¿¡ validationµÊ |
CF™770 | 770 | 797 | DyLight® 800, IRDye® 800CW, ZW800-1 |
• ¹à±â¿Í ¾ÈÁ¤¼ºÀÌ ¸Å¿ì ¶Ù¾î³² • Li-COR Odyssey System¿¡ ȣȯ |
CF™790 | 784 | 806 | Alexa Fluor® 790 | • ¹à±â¿Í ¾ÈÁ¤¼ºÀÌ ¸Å¿ì ¶Ù¾î³² • Highly water soluble without bearing excessive charge |
CF™800 | 797 | 816 | Spectrally similar to Indocyanine green | • Unique long wavelength near-infrared dye |
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