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 제품안내  분자생물학   Genomic Research  Transfection Reagent

EzWay™ Transfection Reagent

EzWay™ Transfection Reagent는 cationic lipid 성분의 transfection reagent로, liposome-DNA complex를 쉽고 빠르게 준비할 수 있으며, 다른 liposomal reagent에 비해 강한 DNA-binding affinity와 높은 transfection efficiency를 보여줍니다.

KOMABIOTECH
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    특징
  • Cationic Lipid 성분의 Transfection Reagent입니다.
  • Plasmid DNA와 RNAi vector의 cell로의 transfection이 용이합니다.
  • Stems cell에서도 transfection이 용이합니다.
  • Serum의 유무에 관계없이 transfection efficiency가 높습니다.
  • 세포 독성이 없어 중간에 media를 교체 해주지 않아도 되며, 세포 viability가 매우 높습니다.
  • in vitroin vivo 모두에 보편적으로 사용할 수 있습니다.
  • Lipoplex (liposome & DNA mixture)를 만들기가 쉽습니다.
  • Enhancer와 혼합할 필요가 없는 one-step 용액 입니다.
  • 24-well plate 기준으로 250 transfection을 할 수 있는 충분한 양이 제공됩니다.
  • Patent No. : 2003-0075611
    Cell Line List
    Cell Line Cell Type
    HeLa Human cervix adenocarcinoma
    293A Human kidney cancer
    B16BL6 Mouse skin melanoma
    B16F10 Mouse skin melanoma
    MCF-7 Human breast adenocarcinoma
    HepG2 Human liver/hepatocellular carcinoma
    Hep-3B Human hepatocellular carcinoma
    Chang Human normal liver cell
    BT-20 Human breast carcinoma
    TC-1 Mouse lung tumor
    CT-26 Mouse colon carcinoma
    EMT-6 Mouse breast cancer
    SK-OV-3 Human ovarian adenocarcinoma
    A549 Human lung epithelial carcinoma
    MDA-MB-231 Human breast adenocarcinoma
    NCI Human lung cancer (carcinoma)
    SK-Br-3 Human breast carcinoma
    HT-29 Human colorectal adenocarcinoma
    Jurkat Human T-cell leukemia
    적용
  • Plasmid Transfection
  • RNAi or siRNA Transfection
  • Stem Cell Transfection
    실험과정
    EzWay™ Transfection Reagent 실험과정
    사용예
  • Efficient delivery of DNA to a wide variety of cell lines
    (A)
    (B)
    Using the EzWay™ Transfection Reagent or competitor reagent L, cells were transfected with the pDNA, which expresses luciferase (A) and GFP (B) respectively. One microgram of pDNA complexed to EzWay ™(1:4.9 wt ratios of pDNA and lipid) was added to a various cell lines, The cells were transfected for 4 hours and incubated for additional 24 hours. The luciferase activity (A) of the cell lysate was measured with a luminometer and a luciferase assay kit and the GFP expression (B) was determined by fluorescence microscopy.
  • Knockdown efficacy of EzWayTM Transfection Reagent
    siRNA targeting luciferase was transfected by EzWay™ Transfection Reagent and competitor reagent L into Hep-3B cells. For B16BL6 cells, siRNA targeting luciferase was co-transfected with luciferase gene by two products, respectively. Luciferase-silencing efficiency was calculated relative to untreated cells. (A) shows the luciferase expression, while (B) shows the reduction in luciferase activity. EzWay™ Transfection Reagent delivered significant gene silencing from 10 nM of renilla luciferase siRNA.
  • Inhibition of GFP expression in cells transfected with GFP targeting siRNA using EzWayTM Transfection Reagent
    MDA-MB-231 cells constitutively expressing the GFP gene were transfected with 10 nM final siRNA concentration of GFP-targeting siRNA.
주문정보(Ordering Information)
CATNUM PRODUCT SIZE
K21000EzWay Transfection Reagent0.5ml
K21005EzWay Transfection Reagent5ml (1mlx5)