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Biotium |
DASPEI is used to stain mitochondria in live cells. Absorption/emission = 461/589 nm (MeOH).
Store at 4 °C and protect from light, especially in solution.
C17H21IN2. Mwt: 380. [3785-01-1].
Dihydrorhodamine 123 is the reduced form of rhodamine 123 (70010), which is a commonly used fluorescent mitochondrial dye. Dihydrorhodamine 123 itself is nonfluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes.1 Dihydrorhodamine 123 is useful for detecting reactive oxygen species including superoxide (in the presence of peroxidase or cytochrome c)2,3 and peroxynitrite.4,5
White solid soluble in DMSO. Store at -20 °C and protect from air and light, especially when in solution.
C20H18N2O3. Mwt: 346.
Also see dihydrorhodamine 123 dihydrochloride (10056), a more stable and water soluble form of dihydrorhodamine 123
Only available from BioTeam, dihydrorhodamine 123 dihydrochloride is the HCl salt form of dihydrorhodamine 123 (10055) and is functionally equivalent to the latter. However, dihydrorhodamine 123 dihydrochloride is more water soluble and more stable to air oxidation than dihydrorhodamine 123. Thus, dihydrorhodamine 123 dihydrochloride is easier to use and can be stored longer than dihydrorhodamine 123.
White solid soluble in DMSO and water. Store at -20 °C and protect from air and light, especially when in solution
C20H20Cl2N2O3. Mwt: 419.
A mitochondrial dye (full chemical name: 5,5' ,6,6' -tetrachloro-1,1' ,3,3' -tetraethyl- benzimidazolylcarbocyanine chloride) that stains mitochondria in living cells in a membrane potential-dependent fashion. JC-1 monomer is in equilibrium with so-called J-aggregates, which are favored at higher dye concentration or higher mitochondrial membrane potential.
The monomer JC-1 has green fluorescence (λ em = 527 nm), while the J-aggregates have red fluorescence (λ em = 590 nm). Therefore, it has been possible to use fluorescence ratioing technique to study mitochondrial membrane potentials.
e (MeOH) = 190,000.
Red solid soluble in DMSO. Store at 4 ° C. Protect from light, especially when in solution.
C25H27Cl3N4. Mwt: 561.5.
JC-1 iodide is similar to JC-1 chloride salt (70011). See 70011 for applications and references.
C25H27Cl4IN4. Mwt: 652 [47729-63-5].
A mitochondrion-staining dye whose staining is not dependent on mitochondrial membrane potential, unlike JC-1 and rhodamine 123 (See 70010 and 70011).
Orange solid soluble in DMSO and DMF. Stable at room temperature. Store at 4 ° C. Protect from light, especially when in solution.
λ ex¡¬λ em (MeOH) = 495nm¡¬522nm. e (MeOH) = 63,000.
C26H38BrN3. Mwt: 473.
A popular green fluorescent mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. Widely used in flow cytometry studies involving mitochondrial membrane potential.
λ ex¡¬λ em (MeOH) = 505nm\534nm. e (505 nm, MeOH) = 97,000.
Orange red solid soluble in DMSO and DMF. Store at 4 ° C. Protect from light, especially when in solution.
C21H17ClN2O3. Mwt: 381. [62669-70-9]
TMRM (70017) and TMRE (70016) are said to be the preferred dyes for quantitative measurements of membrane potentials using Nernst equation. The dyes do not form aggregates in cell membarnes and interact with membrane proteins minimally. Thus, the transmembrane distribution of the dyes is directly related to the membrane potential via Nernst equation.
Absorption/Emission = 549/574nm(MeOH). Red solid soluble in DMSO, DMF or EtOH. Store at 4 °C and protect from light.
C26H27ClIN2O7. Mwt: 515.
TMRM (70017) and TMRE (70016) are said to be the preferred dyes for quantitative measurements of membrane potentials using Nernst equation. The dyes do not form aggregates in cell membarnes and interact with membrane proteins minimally. Thus, the transmembrane distribution of the dyes is directly related to the membrane potential via Nernst equation.
Absorption/emission = 548/573nm(MeOH). Red solid soluble in DMSO, DMF or EtOH. Store at 4 °C and protect from light.
C25H25ClIN2O7. Mwt: 501.
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