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 제품안내  형광시약   Fluorescent Membrane/Membrane Potential Dyes

Membrane Potential Dyes

세포막전위 변화 (membrane potential change)에 따른 dye의 반응도를 관찰하는데 이용되는 형광 dye입니다.

Di-4-ANEPPS는 neuronal membrane potential 연구에, DiBAC은 High Throughput Drug Screening (HTS)에 효과적입니다.

Biotium
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    제품 종류
  • Di-4-ANEPPS (purity > 95% by HPLC)
    • Catalog Number: 61010
    • Di-4-ANEPPS is a fast-responding membrane potential dye. The changes in the fluorescence excitation intensities of the dye are correlated to the changes in the membrane potential of the cell.

      Orange red solid soluble in DMF, ethanol and DMSO. Store at 4 ° C. Protect from light, especially when in solution.

      C28H36N2O3S. Mwt: 481.

    • References
      1. Davidenko, J.M., et al. Nature 355, 349(1992);
      2. Fromherz, P., et al. Biochim. Biophys. Acta 1068, 149(1991).
  • DiBAC4(3) (Full chemical name:bis-(1,3-dibarbituric acid)-trimethine oxanol) (purity > 95% by HPLC)
    • Catalog Number: 61011
    • DiBAC4(3) is a so-called translational membrane potential dye that redistributes within the cell membrane when membrane potential changes.1,2 The fluorescence of the dye is enhanced when the dye enters the cell membrane as a result of membrane depolarization. The rate of fluorescence response of the dye to membrane potential change is usually slower than that of the styryl dye di-4-ANEPPS, but the fluorescence signal change for the former is significantly larger than for the latter dye. A new and potentially very important application for DiBAC4(3) is its use in high throughput drug screening.3

      Orange solid soluble in DMSO or DMF. Store at -20 ° C and protect from light.

      C27H39N4O6. Mwt: 519.

    • References
      1. Chem. Phys. Lipids 69, 137(1994);
      2. Biochim. Biophys. Acta 771, 208(1984);
      3. J. Biomol. Screening 1(2), 75(1996).
  • RH421
    • Catalog Number: 61017
    • RH421 is a fast responding membrane potential dye, exhibiting >20% fluorescence change per 100 mV on neuroblastoma cells.

      Absorption/emision = 515/704 nm (MeOH).

      Red solid soluble in DMSO, EtOH or DMF. Store at -20°C and protect from light, especially in solution.

      C29H42N2O3S. Mwt: 498.72. [107610-19-5].

    • References
      1. Biophys. J. 42, 195(1983).
  • Tetramethylrhodamine ethyl ester, perchlorate (TMRE)
    • Catalog Number: 70016
    • TMRM (70017) and TMRE (70016) are said to be the preferred dyes for quantitative measurements of membrane potentials using Nernst equation. The dyes do not form aggregates in cell membarnes and interact with membrane proteins minimally. Thus, the transmembrane distribution of the dyes is directly related to the membrane potential via Nernst equation.

      Absorption/Emission = 549/574nm(MeOH). Red solid soluble in DMSO, DMF or EtOH. Store at 4 °C and protect from light.

      C26H27ClIN2O7. Mwt: 515.

    • References
      1. Meth. Cell Biol. 38, 195(1993);
      2. Meth. Cell Biol. 30, 193(1989);
      3. Biophys. J. 56, 1053(1989);
      4. Biophys., J. 53, 785(1988).
  • Tetramethylrhodamine methyl ester, perchlorate (TMRM)
    • Catalog Number: 70017
    • TMRM (70017) and TMRE (70016) are said to be the preferred dyes for quantitative measurements of membrane potentials using Nernst equation. The dyes do not form aggregates in cell membarnes and interact with membrane proteins minimally. Thus, the transmembrane distribution of the dyes is directly related to the membrane potential via Nernst equation.

      Absorption/emission = 548/573nm(MeOH). Red solid soluble in DMSO, DMF or EtOH. Store at 4 °C and protect from light.

      C25H25ClIN2O7. Mwt: 501.

    • References
      1. Meth. Cell Biol. 38, 195(1993);
      2. Meth. Cell Biol. 30, 193(1989);
      3. Biophys. J. 56, 1053(1989);
      4. Biophys., J. 53, 785(1988).