• Home
  • About us
  • Log-In
  • Join Us
  • Sitemap
  • Recruit
제품/실험 Q&A
자주묻는질문
자유게시판
실험기법
자료신청
논문자료
클레임 접수
기기 A/S 접수
클레임 진행조회
 제품안내  형광시약   Enzyme Substrates

Substrates For Redox Enzymes

산화환원반응을 이용한 peroxidase substrate와 reductase substrate들을 소개합니다.

Biotium
제품 문의하기
인쇄하기

    제품 종류
  • ABTS(TM) (Full chemical name: 2,2'-azino-bis-(3- ethylbenzothiazoline-6-sulfonic acid), (NH4)2)
    • Catalog Number: 10050
    • A very sensitive chromogenic peroxidase substrate widely used for ELISA applications. The substrate yields a water-soluble green colored product.

      Water-soluble pale yellow solid. Store desiccated at 4 ° C and protect from light.
      C18H24N6O6S4. Mwt: 549. [30931-67-0].

    • References
      1. Meth. Enzymol. 70, 419(1980);
      2. Biochem. J. 145, 93(1975).
  • 2',7'-Dichlorodihydrofluorescein diacetate
    • Catalog Number: 10058
    • H2DCFDA is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation,
      H2DCFDA becomes the highly green fluorescent 2’,7’-dichlorofluorescein

      White solid soluble in DMSO or DMF. Store at -20 °C.
      C24H16Cl2O7. Mwt: 487.26.

    • References
      1. Biochemistry 39, 1040(2000);
      2. Cancer Res. 60, 219(2000);
      3. Free Rad. Res. 32, 57(2000);
      4. J. Immunol. Meth. 159, 173(1993);
      5. J. Immunol. Meth. 159, 131(1993);
      6. Cytometry 13, 615(1992).
  • Dihydroethidium (Also known as Hydroethidium, a trademark of Prescott Labs)
    • Catalog Number: 10057
    • Dihydroethidium (also called Hydroethidium, a trademark of Prescott) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes. Dihydroethidium itself is blue fluorescent (absorption/emission: 355/420nm) in cell cytoplasm while the oxidized form ethidium is red fluorescent (absorption/emission: 518/605 nm) upon DNA intercalation.

      Off-white solid soluble in DMF or DMSO. Store at -20 °C under nitrogen or argon, and protect from light, especially when in solution.
      C21H21N3 Mwt: 315.

    • References
      1. J. Immunol. Meth.. 170, 117(1994);
      2. FEMS Microbiol. Lett. 101, 173(1992);
      3. J. Histochem. Cytochem. 34, 1109(1986);
      4. FEMS Microbiol. Lett. 122, 187(1994).
  • Dihydrorhodamine 123
    • Catalog Number: 10055
    • Dihydrorhodamine 123 is the reduced form of rhodamine 123 (70010), which is a commonly used fluorescent mitochondrial dye. Dihydrorhodamine 123 itself is nonfluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes.1 Dihydrorhodamine 123 is useful for detecting reactive oxygen species including superoxide (in the presence of peroxidase or cytochrome c)2,3 and peroxynitrite.4,5

      White solid soluble in DMSO. Store at -20 °C and protect from air and light, especially when in solution.
      C20H18N2O3. Mwt: 346.

      Also see dihydrorhodamine 123 dihydrochloride (10056), a more stable and water soluble form of dihydrorhodamine 123.

    • References
      1. J. Immunol. Meth. 178, 89(1995);
      2. Eur. J. Biochem. 217, 973(1993);
      3. Arc. Biochem. Biophys. 302, 348(1993);
      4. Biochemistry 34, 3544(1995);
      5. Free Rad. Biol Med. 16, 149(1994).
  • Dihydrorhodamine 123, dihydrochloride salt
    • Catalog Number: 10056
    • Only available from BioTeam, dihydrorhodamine 123 dihydrochloride is the HCl salt form of dihydrorhodamine 123 (10055) and is functionally equivalent to the latter. However, dihydrorhodamine 123 dihydrochloride is more water soluble and more stable to air oxidation than dihydrorhodamine 123. Thus, dihydrorhodamine 123 dihydrochloride is easier to use and can be stored longer than dihydrorhodamine 123.

      White solid soluble in DMSO and water. Store at -20 °C and protect from air and light, especially when in solution.
      C20H20Cl2N2O3. Mwt: 419.

    • References
      1. J. Immunol. Meth. 178, 89(1995);
      2. Eur. J. Biochem. 217, 973(1993);
      3. Arc. Biochem. Biophys. 302, 348(1993);
      4. Biochemistry 34, 3544(1995);
      5. Free Rad. Biol Med. 16, 149(1994).
  • MTT
    • Catalog Number: 10059
    • MTT is a tetrazolium salt that is commonly used to detect reductive metabolism in cells for viability, proliferation and cytotoxicity assays. On reduction tetrazolium salts turn into deeply colored products that can be easily quantified by colorimetric measurement. MTT forms a purple formazan product that needs to be solubilized prior to quantification. MTT and XTT(10060) are particularly useful for HTS of antiviral and anticancer compounds and for the studies of cytokine effect on cell proliferation.

    • References
      1. Antimicro Agents Chemother. 36, 1619(1992);
      2. Cancer Res. 48, 4827(1988);
      3. Parasitology 107, 175(1993);
      4. J. Immunol. Meth. 147, 153(1992).
  • XTT (Full chemical name: 2,3-bis-(2-methoxy-4-nitro- 5-sulfophenyl)-2H-tetrazolium-5-carboxanilide, Na2)
    • Catalog Number: 10060
    • A tetrazolium derivative used widely in cell viability and proliferation testings. The material is readily reduced in viable cells to a highly water-soluble orange colored product (l = 475 nm). Applications of the reagent include studies of : antifungal susceptibility,1 drug sensitivity of cells,2 parasitic nematode viability3 and tumor cell cytotoxicity.4

      Pale yellow solid soluble in water. Store desiccated at 4 ° C.
      C22H12N7NaO13S2. Mwt: 675. [111072-31-2].

    • References
      1. Antimicro Agents Chemother. 36, 1619(1992);
      2. Cancer Res. 48, 4827(1988);
      3. Parasitology 107, 175(1993);
      4. J. Immunol. Meth. 147, 153(1992).