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 제품안내  형광시약   Enzyme Substrates

Substrates For beta-galactosidase

lactase라고도 불리우는 b-Galactosidase는 예로부터 유도성 효소의 전형으로 알려져 왔습니다. lacZ 구조 유전자는 클로닝벡터나 진핵생물세포, 개체에 대한 유전자 도입을 위한 마커로서 널리 사용되고 있는데, 이때 기질로서 푸르게 발색하는 X-gal을 사용합니다. 이외에도 green, purple, red 계열의 새로운 X-gal을 만나보십시요.

Biotium
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    제품 종류
  • Green-beta-D-Gal (Full chemical name: N-methylindolyl- beta-D-galactopyranoside) (purity > 98%)
    • Catalog Number: 10015
    • Chromogenic substrate for β-galactosidase. Similar to X-Gal, but generates green colored precipitate on enzyme hydrolysis.

      White solid soluble in water and DMSO. Store at -20 ° C and protect from light.
      C15H19NO6. Mwt: 309.3.

    • References
      1. Sambrook, et al. Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, B. 14, p186 (1989).
  • Purple-beta-D-Gal (Full chemical name: 5-iodo-3-indolyl -beta-D-galactopyranoside) (purity > 98%)
    • Catalog Number: 10014
    • Chromogenic substrate for β-galactosidase. Similar to X-Gal, but generates a purple colored precipitate on enzyme hydrolysis.

      Off-white solid soluble in water and DMSO. Store at -20 ° C and protect from light.
      C14H16INO6. Mwt: 421.6.

    • References
      1. Sambrook, et al. Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, B. 14, p186 (1989).
  • Red-beta-D-Gal (Full chemical name: 5-bromo-6-chloro -3-indolyl-beta-D-galactopyranoside) (purity > 98%)
    • Catalog Number: 10012
    • Chromogenic substrate for β-galactosidase. Similar to X-Gal, but generates a red or magenta colored precipitate on enzyme hydrolysis.

      White solid soluble in water and DMSO. Store at -20 ° C and protect from light.
      C14H15BrClNO6. Mwt: 408.6. [93863-88-8].

    • References
      1. Sambrook, et al. Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, B. 14, p186 (1989).
  • Rose-beta-D-Gal (6-chloro-3-indoxyl-b-D-galactopyranoside)
    • Catalog Number: 10013
    • Rose-ß -D-Gal chromogenic substrate for ß-galactosidase. The product is similar to X-gal, but generates an intense pink colored precipitate on enzymatic hydrolysis. Protocol for using the material is similar to that for X-gal (See references listed below).

      • lmax = 542 nm
      • White solid soluble in DMSO
      • Store at -20℃ and protect from light
      • C14H16ClNO6.
      • Mwt: 329.6

    • References
      1. Molecular Cloning: A laboratory Manual, Cold Spring Harbor Laboratory, B. 14, p186(1989);
      2. Biotechniques, 27, 438(1999);
      3. Oncogen, 10, 2323(1995);
      4. Devel. Biol., 161, 77(1994);
      5. J. Histochem. Cytochem., 42, 1299(1994);
      6. BioTechniques, 15, 974(1993);
      7. BioTechniques, 15, 292(1993).
  • X-Gal (Full chemical name: 5-bromo-4-chloro-3-indolyl-beta-galactopyranoside)
    • Catalog Number
      • 10011
      • 10011-1: X-Gal (special packaging)
      • 10011-2: X-Gal (special packaging)
    • The most widely used chromogenic substrate for b-galactosidase. Enzyme hydrolysis of the substrate yields a dark blue colored precipitate.

      White solid soluble in water. Store at -20 ° C and protect from light.
      C14H15BrClNO6. Mwt: 408.6. [7240-90-6].

    • References
      1. Sambrook, et al. Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, B. 14, p186 (1989).