Phosphatase Substrates

• BCIP, Na (Full chemical name: 5-bromo-4-chloro -3-indoxyl phosphate, Na2 salt)
  (purity > 98%)
  • Catalog Number
    • 10001
    • 10001-1
    • 10001-2

  • The most widely used chromogenic phosphotase substrate, which forms a dark blue precipitate on enzymatic hydrolysis. It is often used with the oxidant NBT (nitro blue tetrazolium chloride) (10008), which facilitates the precipitation. For your convenience, we offer a BCIP/NBT Kit (10003) that contains both reagents with a price lower than the combined prices of the components. Please also see our BCIP derivatives below that yield red and pink precipitates.

    White solid soluble in water. Store at -20 ° C and protect from light.
    C₈H₄BrClNNa₂O₄P× 1.5H2O. Mwt: 397.5. [102185-33-1].

    
  • References
    1. Biotechniques 12, 656(1992);
    2. Histochemistry 58, 203(1978).
• BCIP, toluidine (Full chemical name: 5-bromo-4-chloro-3-indoxyl phosphate, p-toluidine salt) (purity > 98%)
  • Catalog Number
    • 10002
    • 10002-1
    • 10002-2

  • Similar to BCIP/Na (10001), BCIP/toluidine is also a widely used chromogenic phosphotase substrate, which forms a dark blue precipitate on enzymatic hydrolysis.

    Store at -20 ° C and protect from light.
    C₁₅H₁₆BrClN₂O₄P. Mwt: 433.6. [6578-06-9].

    
  • References
    1. Biotechniques 12, 656(1992);
    2. Histochemistry 58, 203(1978).
• BCIP Pink (Full chemical name: 6-chloro-3-indoxyl phosphate, p-toluidine salt) (purity > 98%)
  • Catalog Number: 10006

  • A derivative of BCIP. Unlike BCIP that yields a blue precipitating product, BCIP Pink produces a pink colored precipitate.

    White solid soluble in water. Store at -20 ° C and protect from light.
    C₁₅H₁₇ClN₂O₄P. Mwt: 354.7.

    
• BCIP Red (Full chemical name: 5-bromo-6-chloro -3-indoxyl phosphate, p-toluidine salt) (purity > 98%)
  • Catalog Number: 10004

  • A derivative of BCIP. Unlike BCIP that yields a blue precipitating product, BCIP Red produces a red colored precipitate.

    White solid soluble in water. Store at -20 ° C and protect from light.
    C₁₅H₁₆BrClN₂O₄P. Mwt: 433.6. [6769-80-8].

    
• BCIP/NBT Kit
  • Catalog Number: 10003

  • This kit contains 100 mg each of BCIP sodium salt (10001) and the oxidant NBT (nitro blue tetrazolium, 10008).

• BCIP Pink/NBT Kit
  • Catalog Number: 10007

  • This kit contains 100 mg each of BCIP Pink (10006) and the precipitation inducer NBT (10008).

• BCIP Red/NBT Kit
  • Catalog Number: 10005

  • This kit contains 100 mg each of BCIP Red (10004) and the oxidant NBT (10008).

• MUP, Free Acid (Full chemical name: 4-methylumbelliferyl phosphate, free acid)
  • Catalog Number
    • 10009
    • 10009-1

  • Fluorogenic phosphotase substrate that releases the blue fluorescent 4-methyl-7-hydroxycoumarin (l_ex\l_em = 360\450 nm). Fluorescence detection of phosphotase activity can be highly sensitive. MUP free acid and its salt (See MUP/Na, 10010) are the most widely used fluorogenic substrates for alkaline phosphotase detection.

    White solid soluble in water and DMSO. Store desiccated at -20 ° C and protect from light.
    C₁₀H₉O₆P. Mwt: 256. [3368-04-5].

    
  • References
    1. J. Immuno. Meth. 150, 23(1992);
    2. Anal. Chem. 65, 1147(1993);
    3. Anal. Biochem. 205, 1(1992).
• MUP, Na (Full chemical name: 4-methylumbelliferyl phosphate, disodium salt)
  • Catalog Number
    • 10010
    • 10010-1

  • Sodium salt of MUP. Structure similar to 10009. Its use is similar to that of MUP/free acid. See 10009.

    C₁₀H₇Na₂O₆P× 3H2O. Mwt: 354.2. [22919-26-2].

• NBT (Alternative name: Nitro blue tetrazolium, chloride) (purity > 98%)
  • Catalog Number
    • 10008
    • 10008-1
    • 10008-2

  • NBT is an oxidant and is widely used in conjuction with BCIP or BCIP derivatives to detect phosphotase activity. NBT is also a substrate for dehydrogenase and other oxidases. Thus, it is often used to assess cell viability, proliferation and cytotoxicity, and is also frequently used in histochemistry studies. The colorless NBT is soluble in water, but forms a dark blue-colored, highly insoluble precipitate after being enzymatically reduced.

    White solid soluble in water and DMSO. Store at 4 ° C.
    C₄₀H₃₀Cl₂N₁₀O₆. Mwt: 817.7. [298-83-9].

  • References
    1. Meth. Enzymol. 6, 958(1963);
    2. Anal. Biochem. 56, 353(1973);
    3. J. Immunol. Meth. 179, 95(1995);
    4. J. Infect. Dis. 172, 1153(1995);
    5. J. Appl. Bateriol.74, 433(1993);
    6. J. Immunol. Meth. 65, 55(1983).

• Bioluminescent Substrates
• Peptidase and Protease Substrates
• Substrates For Redox Enzymes
• Substrates For beta-galactosidase