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 제품안내  형광시약   Enzyme Substrates

Phosphatase Substrates

Alkaline phosphatase는 streptavidin, avidin, 항체등에 붙여져 ELISA나 IHC, Northern, Southern, Western blot등 각종 detection 실험에 이용되는 효소입니다. BCIP를 주요기질로 이용하며, 어두운 푸른빛을 띠는 일반 BCIP기질외에, red, pink계열의 새로운 BCIP도 있습니다. 보다 민감도 높은 측정을 위해서는 형광 phosphatase 기질을 이용하시면 됩니다.

Biotium
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    제품 종류
  • BCIP, Na (Full chemical name: 5-bromo-4-chloro -3-indoxyl phosphate, Na2 salt)
    (purity > 98%)
    • Catalog Number
      • 10001
      • 10001-1
      • 10001-2
    • The most widely used chromogenic phosphotase substrate, which forms a dark blue precipitate on enzymatic hydrolysis. It is often used with the oxidant NBT (nitro blue tetrazolium chloride) (10008), which facilitates the precipitation. For your convenience, we offer a BCIP/NBT Kit (10003) that contains both reagents with a price lower than the combined prices of the components. Please also see our BCIP derivatives below that yield red and pink precipitates.

      White solid soluble in water. Store at -20 ° C and protect from light.
      C8H4BrClNNa2O4P× 1.5H2O. Mwt: 397.5. [102185-33-1].

    • References
      1. Biotechniques 12, 656(1992);
      2. Histochemistry 58, 203(1978).
  • BCIP, toluidine (Full chemical name: 5-bromo-4-chloro-3-indoxyl phosphate, p-toluidine salt) (purity > 98%)
    • Catalog Number
      • 10002
      • 10002-1
      • 10002-2
    • Similar to BCIP/Na (10001), BCIP/toluidine is also a widely used chromogenic phosphotase substrate, which forms a dark blue precipitate on enzymatic hydrolysis.

      Store at -20 ° C and protect from light.
      C15H16BrClN2O4P. Mwt: 433.6. [6578-06-9].

    • References
      1. Biotechniques 12, 656(1992);
      2. Histochemistry 58, 203(1978).
  • BCIP Pink (Full chemical name: 6-chloro-3-indoxyl phosphate, p-toluidine salt) (purity > 98%)
    • Catalog Number: 10006
    • A derivative of BCIP. Unlike BCIP that yields a blue precipitating product, BCIP Pink produces a pink colored precipitate.

      White solid soluble in water. Store at -20 ° C and protect from light.
      C15H17ClN2O4P. Mwt: 354.7.

  • BCIP Red (Full chemical name: 5-bromo-6-chloro -3-indoxyl phosphate, p-toluidine salt) (purity > 98%)
    • Catalog Number: 10004
    • A derivative of BCIP. Unlike BCIP that yields a blue precipitating product, BCIP Red produces a red colored precipitate.

      White solid soluble in water. Store at -20 ° C and protect from light.
      C15H16BrClN2O4P. Mwt: 433.6. [6769-80-8].

  • BCIP/NBT Kit
    • Catalog Number: 10003
    • This kit contains 100 mg each of BCIP sodium salt (10001) and the oxidant NBT (nitro blue tetrazolium, 10008).

  • BCIP Pink/NBT Kit
    • Catalog Number: 10007
    • This kit contains 100 mg each of BCIP Pink (10006) and the precipitation inducer NBT (10008).

  • BCIP Red/NBT Kit
    • Catalog Number: 10005
    • This kit contains 100 mg each of BCIP Red (10004) and the oxidant NBT (10008).

  • MUP, Free Acid (Full chemical name: 4-methylumbelliferyl phosphate, free acid)
    • Catalog Number
      • 10009
      • 10009-1
    • Fluorogenic phosphotase substrate that releases the blue fluorescent 4-methyl-7-hydroxycoumarin (lex\lem = 360\450 nm). Fluorescence detection of phosphotase activity can be highly sensitive. MUP free acid and its salt (See MUP/Na, 10010) are the most widely used fluorogenic substrates for alkaline phosphotase detection.

      White solid soluble in water and DMSO. Store desiccated at -20 ° C and protect from light.
      C10H9O6P. Mwt: 256. [3368-04-5].

    • References
      1. J. Immuno. Meth. 150, 23(1992);
      2. Anal. Chem. 65, 1147(1993);
      3. Anal. Biochem. 205, 1(1992).
  • MUP, Na (Full chemical name: 4-methylumbelliferyl phosphate, disodium salt)
    • Catalog Number
      • 10010
      • 10010-1
    • Sodium salt of MUP. Structure similar to 10009. Its use is similar to that of MUP/free acid. See 10009.

      C10H7Na2O6P× 3H2O. Mwt: 354.2. [22919-26-2].

  • NBT (Alternative name: Nitro blue tetrazolium, chloride) (purity > 98%)
    • Catalog Number
      • 10008
      • 10008-1
      • 10008-2
    • NBT is an oxidant and is widely used in conjuction with BCIP or BCIP derivatives to detect phosphotase activity. NBT is also a substrate for dehydrogenase and other oxidases. Thus, it is often used to assess cell viability, proliferation and cytotoxicity, and is also frequently used in histochemistry studies. The colorless NBT is soluble in water, but forms a dark blue-colored, highly insoluble precipitate after being enzymatically reduced.

      White solid soluble in water and DMSO. Store at 4 ° C.
      C40H30Cl2N10O6. Mwt: 817.7. [298-83-9].

    • References
      1. Meth. Enzymol. 6, 958(1963);
      2. Anal. Biochem. 56, 353(1973);
      3. J. Immunol. Meth. 179, 95(1995);
      4. J. Infect. Dis. 172, 1153(1995);
      5. J. Appl. Bateriol.74, 433(1993);
      6. J. Immunol. Meth. 65, 55(1983).