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 제품안내  형광시약   Fluorescent Ion Indicators

Calcium Indicators

Ca2+은 세포내 활성-불활성에 관여하는 중요한 messenger ion중의 하나입니다. 따라서 세포내 Ca2+의 양의 측정은 세포메카니즘에서의 Ca2+의 역할을 규명하는데 결정적이며, 주로 형광탐식자(Fluorescent probe)를 이용하여 그 양을 측정합니다.

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    사양
  • Ca2+ Indicator의 종류
    • Fluo-3
      • Argon ion laser에 사용됩니다.
      • 칼슘과 결합하면 500-550 nM의 파장에서 감지됩니다.
      • 칼슘과의 binding affinity가 아주 높지는 않으나, 일단 binding이 포화되면 형광도가 수백배 증가합니다.
      • 급격한 농도의 칼슘변화를 측정할때 이용됩니다.
    • Rhod-2
      • Fluo-3와 같은 파장입니다.
      • Positive charge를 띠고 있어, mitochondria로 쉽게 들어가므로 mitochondria 내의 칼슘 측정에 용이하여 apoptosis 연구에 적합합니다.
    • Fura-2
      • UV에 사용됩니다.
      • 칼슘과 결합하지 않을 때는 380nM, 결합했을 때는 340nM로 두 파장의 변화비율에 따라 칼슘 농도가 측정됩니다.
      • 칼슘과의 binding affinity가 매우 높아 아주 미세한 칼슘 농도 변화를 측정할 수 있습니다.
      • 칼슘 이외의 다른 2가 이온과도 결합하나, 칼슘과의 반응성이 제일 높습니다.
    • Furaptra (Mag-Fura-2)
      • Fura-2와 같은 파장입니다.
      • 칼슘 보다는 다른 2가 이온(Mg2+, Zn2+ 등)과의 반응성이 더 높아 이들의 측정에 사용됩니다.
    • Indo-1
      • UV에 사용됩니다.
      • 칼슘과 결합하지 않을 때는 482nM, 결합했을 때는 398nM로 두 파장의 변화비율에 따라 칼슘 농도가 측정됩니다.
      • Fura-2보다 파장 변화가 높아 측정하기 어려운 부위(membrane 등)에 주로 사용됩니다.
    제품 종류
  • A-23187 Free Acid (calcimycin; or Calcium Ionophore III)
    • Catalog Number: 59001
    • A-23187 is a calcium ionophore that rapidly equilibrates intracellular and extracellular calcium concentrations. It is commonly used for in situ calibrations of fluorescent calcium indicators. The ionophore allows Mn2+ to enter the cells and quench intracellular fluorescence of calcium indicators.

      White solid soluble in DMSO. Store at 4 °C and protect from light.
      C29H37N3O6. Mwt: 523.6. [52665-69-7].

    • References
      1. Suzuki, K. et al. Anal. Chem. 61, 382 (1989);
      2. Caron-Leslie, L.A., et al. FASEB J. 8, 639 (1994);
      3. Merck Index 12, 1678;
      4. Saunders, M.J., et al. Science 217, 943 (1982).
  • Calcium Calibration Buffer
    • Catalog Number: 59100
    • This kit contains a 10 mM K2EGTA buffered solution and a 10 mM CaEGTA buffered solution. The kit can be used to generate calcium concentrations from zero up to 40 mM
      Store desiccated at 4 °C.

  • EDC (EDAC, 1-(3-dimethylaminopropyl-3-ethylcarbodiimide, hydrochloride)
    • Catalog Number: 59002
    • EDC (or EDAC) has recently been found to be very useful for fixing in situ chelators, including the fluorescent ion indicators in this catalogue. The fixation of ion indicators makes it useful for post histological studies following the physiological experiments. EDC is also a widely used reagent to activate carboxy groups for amine couplings.

      White solid soluble in water. Store desiccated at -20 °C.
      8H18ClN3. Mwt: 191.7. [25952-53-8].

    • References
      1. Tymianski, M. et al. Cell Calcium 21(3), 175(1997);
      2. Tetrahedron Lett. 34, 7685(1993).
  • Fluo-3, AM ester (purity > 95% by HPLC)
    • Catalog Number: 50013
    • Membrane-permeant form and thus can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127 (See 59000 and refs. therein), a mild detergent, is often used as a dispersing agent to facilitate the loading. Fluo-3/AM itself does not bind Ca2+, but it is readily hydrolyzed to fluo-3 by endogenous esterases once the dye is inside the cells. A new and potentially very important application of Fluo-3/AM is its use in high throughput drug screening.

      Orange red solid soluble in DMSO. Keep frozen at -20 ° C. Protect from light, especially when in solution.
      C51H50Cl2N2O23. Mwt: 1129.9. [121714-22-5]. For other information on fluo-3, see 50010.

      For your convenience, this product is packaged in 100 m g vials.

    • References
      1. Niggli, E., et al. Cell Calcium 11, 121(1990);
      2. Kao, J.P.Y., et al. J. Cell Biol. 111, 183(1990);
      3. Schuman, M.A., et al. J. Biol. Chem. 268, 2134(1993);
      4. Lattanzio, F.A., et al. Biochem. Biophys. Res. Comm. 171, 102(1990);
      5. Schroeder et al. J. Biomol. Screening 1(2), 75(1996).
  • Fluo-3, AM ester (purity > 95% by HPLC)
    • Catalog Number
      • 50014: Fluo-3, AM ester(purity: >95% by HPLC)
      • 50015: Fluo-3, AM ester(1mM in anhydrous DMSO)
      • 50016: Fluo-3, AM ester(special packaging)
    • Membrane-permeant form and thus can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127 (See 59000 and refs. therein), a mild detergent, is often used as a dispersing agent to facilitate the loading. Fluo-3/AM itself does not bind Ca2+, but it is readily hydrolyzed to fluo-3 by endogenous esterases once the dye is inside the cells. A new and potentially very important application of Fluo-3/AM is its use in high throughput drug screening.

      Orange red solid soluble in DMSO. Keep frozen at -20 ° C. Protect from light, especially when in solution.
      C51H50Cl2N2O23. Mwt: 1129.9. [121714-22-5]. For other information on fluo-3, see 50010.

      For your convenience, this product is also packaged in 100 m g vials (see 50013).

    • References
      1. Niggli, E., et al. Cell Calcium 11, 121(1990);
      2. Kao, J.P.Y., et al. J. Cell Biol. 111, 183(1990);
      3. Schuman, M.A., et al. J. Biol. Chem. 268, 2134(1993);
      4. Lattanzio, F.A., et al. Biochem. Biophys. Res. Comm. 171, 102(1990);
      5. Schroeder et al. J. Biomol. Screening 1(2), 75(1996).
  • Fluo-3, pentaammonium salt
    • Catalog Number: 50010
    • Membrane-impermeant but can be loaded into cells via microinjection or scrape loading.
      Kd: 450 nM. λ ex\λem (low or high [Ca2+]) = 506nm/526nm.e(506 nm) = 86,000 M-1cm-1

      Orange red solid soluble in DMSO and water (pH >6). Store at 4 ° C. Protect from light, especially when in solution.
      C36H45Cl2N7O13. Mwt: 854.7. [134907-84-9].

    • References
      1. Zucker, R.S., et al. Cell Calcium. 13, 29(1992);
      2. Merritt, J.E., et al. Biochem. J. 269, 513(1990);
      3. Lattanzio, F.A., et al. Biochem. Biophys. Res. Comm. 171, 102(1990);
      4. Jaffe, L. et al. Proc. Natl. Acad. Sci. USA 88, 9883(1991).
  • Fluo-3, pentapotassium salt
    • Catalog Number: 50011
    • Membrane-impermeant but can be loaded into cells via microinjection or scrape loading. Kd: 450 nM. l ex\l em(low or high [Ca2+]) = 506nm/526nm. e (506 nm) = 86,000 M-1cm-1.

      Orange red solid soluble in DMSO and water (pH >6). Store at 4 ° C. Protect from light, especially when in solution.
      C36H25Cl2K5N7O13. Mwt: 960.
      Structure similar to 50010.

  • Fluo-3, pentasodium salt
    • Catalog Number: 50012
    • Membrane-impermeant but can be loaded into cells via microinjection or scrape loading. Kd: 450 nM. λ ex\λ em (low or high [Ca2+]) = 506nm/526nm. e (506 nm) = 86,000 M-1cm-1.

      Orange red solid soluble in DMSO and water (pH >6). Store at 4 ° C. Protect from light, especially when in solution.
      C36H25Cl2N7Na5O13. Mwt: 880.
      Structure similar to 50010.

  • Fura-2, AM ester
    • Catalog Number
      • 50033: Fura-2, AM ester(purity: >98% by HPLC)
      • 50033-1: Fura-2, AM ester(purity: >98% by HPLC)
      • 50034: Fura-2, AM ester(purity: >98% by HPLC)
    • Membrane-permeant form and thus can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127 (See 59000 and refs. therein), a mild detergent, is often used as a dispersing agent to facilitate the loading. Fura-2/AM itself does not bind Ca2+, but it is readily hydrolyzed to Fura-2 by endogenous esterases once the dye is inside the cells. Light yellow solid soluble in DMSO.

      Store at -20°C and protect from light, especially when in solution. C44H47N3O24. Mwt: 1001.9. [108964-32-5]. For other information on Fura-2, see 50030.

    • References
      1. Davis, M.J., et al. Am. J. Physiol. 263, 1292(1992)
      2. Ward, C.A. and Moffat, M.P. J. Mol. Cell. Cardiol. 24, 937(1992).
  • Fura-2, pentaammonium salt
    • Catalog Number: 50030
    • Membrane-impermeant but can be loaded into cells via microinjection or scrape loading. Kd: 224 nM. λex\λem (no Ca2+) = 363nm/512nm; λex\λem(high Ca2+) = 335nm/505nm. ε(363nm, no Ca2+) = 27,000 M-1cm-1; e (335nm, high Ca2+) = 35,000 M-1cm-1. Light yellow solid soluble in DMSO.

      Store at 4℃. Protect from light, especially when in solution. C29H37N8O14. Mwt: 721.

    • References
      1. Davis, M.J., et al. Am. J. Physiol. 263, 1292(1992)
      2. Ward, C.A. and Moffat, M.P. J. Mol. Cell. Cardiol. 24, 937(1992).
  • Furaptra, AM ester (also known as Mag-Fura-2, AM ester) (purity > 95% by HPLC)
    • Catalog Number: 50037
    • Membrane-permeant form of Mag-Fura-2. The indicator is loaded into cells via simple incubation of cells in the buffer containing the dye. See information for Mag-Fura-2/tetrapotassium salt (50035).

      Light yellow solid soluble in DMSO. Store at -20 ° C and protect from light.
      C30H30N2O19. Mwt: 723.

      For your convenience, this product is packaged in 100 μg vials.

  • Furaptra, AM ester (also known as Mag-Fura-2, AM ester) (purity > 95% by HPLC)
    • Catalog Number: 50038
    • Membrane-permeant form of Mag-Fura-2. The indicator is loaded into cells via simple incubation of cells in the buffer containing the dye. See information for Mag-Fura-2/tetrapotassium salt (50035).

      Light yellow solid soluble in DMSO. Store at -20 ° C and protect from light.
      C30H30N2O19. Mwt: 723.

      For your convenience, this product is also packaged in 100 μg vials (See 50037) and 50 μg vials (See 50039)

  • Furaptra, AM ester (also known as Mag-Fura-2, AM ester) (purity > 95% by HPLC)
    • Catalog Number: 50039
    • Membrane-permeant form of Mag-Fura-2. The indicator is loaded into cells via simple incubation of cells in the buffer containing the dye. See information for Mag-Fura-2/tetrapotassium salt (50035).

      Light yellow solid soluble in DMSO. Store at -20 ° C and protect from light.
      C30H30N2O19. Mwt: 723.

      For your convenience, this product is packaged in 50 μg vials.

  • Furaptra, tetrapotassium salt (also known as Mag-Fura-2, tetrapotassium salt)
    • Catalog Number: 50035
    • Mag-Fura-2 is a UV-excitable fluorescent indicator for magnesium with a Kd of 1.9 mM.1,2 Similar to Fura-2, the excitation wavelength of Mag-Fura-2 undergoes a blue shift from 369 nm to 330 nm. Mag-Fura-2 also responds to Ca2+ but with a significantly lower Kd than Fura-2 to Ca2+. An important application of Mag-Fura-2 is its use in detecting high, transient Ca2+ concentration during Ca2+ spikes.3,4 Mag-Fura-2/tetraammonium salt can be loaded into cells by microinjection or scrape loading.

      Light yellow solid soluble in water (pH > 6). Store at -20 ° C and protect from light.
      C18H10K4N2O11. Mwt: 587.

    • References
      1. Pro. Natl. Acad. Sci.USA 86, 2981(1989);
      2. Am. J. Physiol. 256, C540(1989);
      3. Neuron 10, 21(1993);
      4. Biophys. J. 68, 2156(1995).
  • Furaptra, tetrasodium salt (also known as Mag-Fura-2, tetrasodium salt)
    • Catalog Number: 50036
    • Sodium salt of Mag-Fura-2 similar to Mag-Fura-2/tetrapotassium salt (50035).

      Light yellow solid soluble in water (pH > 6). Store at -20 ° C and protect from light. C18H10N2Na4O11. Mwt: 523
      Structure similar to 50035.

    • References
      1. Pro. Natl. Acad. Sci.USA 86, 2981(1989);
      2. Am. J. Physiol. 256, C540(1989);
      3. Neuron 10, 21(1993);
      4. Biophys. J. 68, 2156(1995).
  • bis-Fura-2, hexapotassium salt
    • Catalog Number: 50045
    • Bis-Fura-2, hexapotassium salt is membrane-impermeant derivative of membrane-permeant calcium indicator Fura-2, AM ester. It contains two Fura-2-pype fluorophores. This indicator has a slightly lower infinity for Ca2+ and can be introduced into cell by microinjection.

    • References
      1. J Neurophysiol 84, 2777(2000);
      2. Brain Res 831, 113(1999);
      3. J Neurophysiol 81, 2508(1999);
      4. J Neurosci Res 57, 906(1999);
      5. J. Neurochem Int 34, 391(1999)
  • Rhod-2, AM ester (purity > 95% by HPLC)
    • Catalog Number: 50023
    • Membrane-permeant form and thus can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127 (See 59000 and refs. therein), a mild detergent, is often used as a dispersing agent to facilitate the loading. Rhod-2/AM itself does not bind Ca2+, but it is readily hydrolyzed to rhod-2 by endogenous esterases once the dye is inside the cells.

      Red solid soluble in DMSO. Keep frozen at -20 ° C. Protect from light, especially when in solution.
      C52H59BrN4O19. Mwt: 1132.
      For other information on rhod-2, see 50020.

      For your convenience, this product is packaged in 100 μg vials.

    • References
      1. Meyer, T., et al. Biochemistry 29, 32(1990);
      2. Minta, A., et al. J. Biol. Chem. 264, 8171(1989).
  • Rhod-2, AM ester (purity > 95% by HPLC)
    • Catalog Number: 50024
    • Membrane-permeant form and thus can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127 (See 59000 and refs. therein), a mild detergent, is often used as a dispersing agent to facilitate the loading. Rhod-2/AM itself does not bind Ca2+, but it is readily hydrolyzed to rhod-2 by endogenous esterases once the dye is inside the cells.

      Red solid soluble in DMSO. Keep frozen at -20 ° C. Protect from light, especially when in solution.
      C52H59BrN4O19. Mwt: 1132.
      For other information on rhod-2, see 50020.

      For your convenience, this product is also packaged in 100 μg vials (See 50023).

    • References
      1. Meyer, T., et al. Biochemistry 29, 32(1990);
      2. Minta, A., et al. J. Biol. Chem. 264, 8171(1989).
  • Rhod-2, triammonium salt
    • Catalog Number: 50020
    • Membrane-impermeant but can be loaded into cells via microinjection or scrape loading.

      Kd: 1 mM. λ ex\λ em (low or high [Ca2+]) = 556nm/576nm. e (556 nm) = 80,000 M-1cm-1.
      Orange red solid soluble in DMSO and water (pH >6). Store at 4 ° C. Protect from light, especially when in solution.
      C40H51N7O11. Mwt: 930.

    • References
      1. Vergara, J., et al. Biophys. J. 59, 12(1991);
      2. Parker, I., et al. Science 250, 977(1990);
      3. Minta, A., et al. J. Biol. Chem. 264, 8171(1989)
  • Rhod-2, tripotassium salt
    • Catalog Number: 50021
    • Membrane-impermeant but can be loaded into cells via microinjection or scrape loading.

      Kd: 1 mM. λ ex\λ em (low or high [Ca2+]) = 556nm/576nm. e (556 nm) = 80,000 M-1cm-1.
      Orange red solid soluble in DMSO and water (pH >6). Store at 4 ° C. Protect from light, especially when in solution.
      C40H39K3N4O11. Mwt: 993.
      Structure similar to 50020.

    • References
      1. Vergara, J., et al. Biophys. J. 59, 12(1991);
      2. Parker, I., et al. Science 250, 977(1990);
      3. Minta, A., et al. J. Biol. Chem. 264, 8171(1989)
  • Rhod-2, trisodium salt
    • Catalog Number: 50022
    • Membrane-impermeant but can be loaded into cells via microinjection or scrape loading.

      Kd: 1 mM. λ ex\λ em (low or high [Ca2+]) = 556nm/576nm. e (556 nm) = 80,000 M-1cm-1.
      Orange red solid soluble in DMSO and water (pH >6). Store at 4 ° C. Protect from light, especially when in solution.
      C40H39N4Na3O11. Mwt: 945.
      Structure similar to 50020.

    • References
      1. Vergara, J., et al. Biophys. J. 59, 12(1991);
      2. Parker, I., et al. Science 250, 977(1990);
      3. Minta, A., et al. J. Biol. Chem. 264, 8171(1989)
  • Fluonic F-127 (Free sample per order of any AM esters)
    • Catalog Number: 59000
    • Nonionic detergent useful for solubilizing large dye molecules such as Fura-2/AM, Fluo-3/AM, Indo-1/AM and Rhod-2/AM to facilitate cell loading. Pluronic F-127 is usually dissolved in DMSO or water by gentle heating.

      Pluronic F-127 is a registered trade mark of BASF.

    • References
      1. Poenie, M. et al. Science 233, 886(1986);
      2. Drummond, I.A.S., et al. J. Biol. Chem. 262, 12801(1987).
주문정보(Ordering Information)
CATNUM PRODUCT SIZE
590064-Bromo A-23187, free acid1mg
500045,5'-Dibromo BAPTA,tetrapotassium salt100 mg
500055,5'-Difluoro BAPTA,AM ester25 mg
500065,5'-Difluoro BAPTA,tetrapotassium salt100 mg
500075,5'-Dimethyl BAPTA,AM ester25 mg
500085,5'-Dimethyl BAPTA,tetrapotassium salt100 mg
50000BAPTA, AM ester25mg
50000-1BAPTA, AM ester20x1mg
50001BAPTA, tetracesium salt1g
50002BAPTA, tetrapotassium salt1g
50003BAPTA, tetrasodium salt1g
59100Calcium Calibration Buffer Kit (zero and 10 mM CaEGTA, 2x50mL)kit
50050DMNP-EDTA, tetrapotassium salt (Caged Calcium)5 mg
90082DMSO, anhydrous10mL
50014Fluo-3, AM ester1mg
50016Fluo-3, AM ester (special packaging)20x50ug
50015Fluo-3, AM ester, 1mM in anhydrous DMSO1.0mL
50010Fluo-3, pentaammonium salt1mg
50011Fluo-3, pentapotassium salt1mg
50012Fluo-3, pentasodium salt1mg
50034Fura-2, AM ester1mg
50033Fura-2, AM ester (special packaging)10x100ug
50033-1Fura-2, AM ester (special packaging)20x50ug
50029Fura-2, AM ester, 1mM in anhydrous DMSO1ml
50030Fura-2, pentaammonium salt1mg
50031Fura-2, pentapotassium salt1mg
50032Fura-2, pentasodium salt1mg
50038Furaptra, AM ester (also known as Mag-Fura-2, AM ester)1mg
50037Furaptra, AM ester (also known as Mag-Fura-2, AM ester) (special packaging)10x100ug
50039Furaptra, AM ester (also known as Mag-Fura-2, AM ester) (special packaging)20x50ug
50035Furaptra, tetrapotassium salt (also known as Mag-Fura-2, tetrapotassium salt)1mg
50036Furaptra, tetrasodium salt (also known as Mag-Fura-2, tetrasodium salt)1mg
50044Indo-1, AM ester1mg
50043Indo-1, AM ester (special packaging)10x100ug
50043-1Indo-1, AM ester (special packaging)20x50ug
50040Indo-1, pentaammonium salt1mg
50041Indo-1, pentapotassium salt1mg
50042Indo-1, pentasodium salt1mg
59007Ionomycin1mg
59000Pluronic F-1271g
59004Pluronic F-127 20% solution in DMSO1ml
59005Pluronic F-127, 10% in H2O30mL
50027Probenecid, sodium salt *water soluble*10x77 mg
50024Rhod-2, AM ester1mg
50020Rhod-2, triammonium salt1mg
50021Rhod-2, tripotassium salt1mg
50022Rhod-2, trisodium salt1mg