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 Á¦Ç°¾È³»  Ç×ü,Ç׿ø,ŶƮ   Cancer Research

Endothelial Tube Formation Assay (In Vitro Angiogenesis Assay)

ÀϹÝÀûÀ¸·Î ³»ÇǼ¼Æ÷ (endothelial cell)Àº collagenÀ̳ª fibrinÀ¸·Î ÄÚÆÃµÈ dish¿¡¼­ ¼öÀϳ»¿¡ tube¸¦ Çü¼ºÇÒ ¼ö ÀÖ½À´Ï´Ù.

Cell BiolabsÀÇ Endothelial Tube Formation Assay KitÀº EHS(Engelbreth–Holm–Swarm) tumor cell À¯·¡ÀÇ ECMÀ» ÀÌ¿ëÇÏ¿© ³»ÇǼ¼Æ÷ÀÇ tube Çü¼º´ÉÀ» È®ÀÎÇÒ ¼ö ÀÖµµ·Ï °í¾ÈµÈ Á¦Ç°ÀÔ´Ï´Ù.

Cell Biolabs
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  • In vitro¿¡¼­ angiogenic tubeÀÇ Çü¼ºÀ» °üÂûÇÒ ¼ö ÀÖ½À´Ï´Ù.
  • In vivo basement membrane ȯ°æ°ú ºñ½ÁÇÑ ECM matrix gelÀ» ÀÌ¿ëÇÕ´Ï´Ù.
  • 18½Ã°£³»¿¡ tube formationÀ» °üÂûÇÒ ¼ö ÀÖ½À´Ï´Ù.
  • kit¿¡ Á¦°øµÇ´Â Çü±¤ dye¸¦ ÅëÇØ tube ±æÀÌ, branch pointµîÀÇ tube formation Á¤µµ¸¦ È®ÀÎÇÒ ¼ö ÀÖ½À´Ï´Ù.
  • Size : 50 assays
  • Detection : Light Microscopy
  • ECM Gel Solution (Part No. 10010): Five 0.5 mL tubes, Ready-To-Use
  • 10X Staining Buffer (Part No. 10011): One 1 mL tube
  • Staining Dye (Part No. 10012): One 50 µL tube of 1 mM Calcein AM in DMSO

 





¡¡ Recent Product Citations
¡¡ 1. Wolcott, K.M. et al. (2020). CD34 positive cells isolated from traumatized human skeletal muscle require the CD34 protein for multi-potential differentiation. Cell Signal. doi: 10.1016/j.cellsig.2020.109711.
¡¡ 2. Guerrero, F. et al. (2020). Role of endothelial microvesicles released by p-cresol on endothelial dysfunction. Sci Rep. 10(1):10657. doi: 10.1038/s41598-020-67574-6.
¡¡ 3. Kim, H.K. et al. (2019). RRAD expression in gastric and colorectal cancer with peritoneal carcinomatosis. Sci Rep. 9(1):19439. doi: 10.1038/s41598-019-55767-7.
¡¡ 4. Cam, M. et al. (2016). ΔNp63 mediates cellular survival and metastasis in canine osteosarcoma. Oncotarget. 7(30):48533-48546. doi: 10.18632/oncotarget.10406.
¡¡ 5. Shirasu, N. et al. (2019). Highly versatile cancer photoimmunotherapy using photosensitizer-conjugated avidin and biotin-conjugated targeting antibodies. Cancer Cell Int. doi: 10.1186/s12935-019-1034-4.
¡¡ 6. Kitala, D. et al. (2019). Amniotic cells share clusters of differentiation of fibroblasts and keratinocytes, influencing their ability to proliferate and aid in wound healing while impairing their angiogenesis capability. Eur J Pharmacol. pii: S0014-2999(19)30148-7. doi: 10.1016/j.ejphar.2019.02.043.
¡¡ 7. Teng, X. et al. (2019). Selective deletion of endothelial cell calpain in mice reduces diabetic cardiomyopathy by improving angiogenesis. Diabetologia. 62(5):860-872. doi: 10.1007/s00125-019-4828-y.
¡¡ 8. Li, W. et al. (2019). LncRNA OR3A4 participates in the angiogenesis of hepatocellular carcinoma through modulating AGGF1/akt/mTOR pathway. Eur J Pharmacol. 849:106-114. doi: 10.1016/j.ejphar.2019.01.049.
¡¡ 9. Chen, C.Y. et al. (2018). N-Terminomics identifies HtrA1 cleavage of thrombospondin-1 with generation of a proangiogenic fragment in the polarized retinal pigment epithelial cell model of age-related macular degeneration. Matrix Biol. 70:84-101. doi: 10.1016/j.matbio.2018.03.013.
¡¡ 10. Mouritzen, M.V. et al. (2018). Neurotensin, substance P, and insulin enhance cell migration. J Pept Sci. 24(7):e3093. doi: 10.1002/psc.3093.
¡¡ 11. Kudo, H. et al. (2018). A potential role for the silent information regulator 2 homologue 1 (SIRT1) in periapical periodontitis. Int Endod J. 51(7):747-757. doi: 10.1111/iej.12894.
¡¡ 12. Suda, M. et al. (2017). Inhibition of dipeptidyl peptidase-4 ameliorates cardiac ischemia and systolic dysfunction by up-regulating the FGF-2/EGR-1 pathway. PLoS One. 12(8):e0182422. doi: 10.1371/journal.pone.0182422.
¡¡ 13. Bae, W.J. et al. (2017). Lysyl oxidase-mediated VEGF-induced differentiation and angiogenesis in human dental pulp cells. Int. Endod. J. doi: 10.1111/iej.12796.
¡¡ 14. Sakaguchi, K. et al. (2017). Periodontal tissue regeneration using the cytokine cocktail mimicking secretomes in the conditioned media from human mesenchymal stem cells. Biochem Biophys Res Commun. doi: 10.1016/j.bbrc.2017.01.065.
¡¡ 15. Katagiri, W. et al. (2017). A defined mix of cytokines mimics conditioned medium from cultures of bone marrow-derived mesenchymal stem cells and elicits bone regeneration. Cell Prolif. doi: 10.1111/cpr.12333.
¡¡ 16. Kim, J.Y. et al. (2017). Role of Protein Phosphatase 1 in Angiogenesis and Odontoblastic Differentiation of Human Dental Pulp Cells. J Endod. doi: 10.1016/j.joen.2016.10.013.
¡¡ 17. Lee, S.I. et al. (2016). Baicalein promotes angiogenesis and odontoblastic differentiation via the BMP and Wnt pathways in human dental pulp cells. Am. J. Chinese Med. 44:1457.
¡¡ 18. Yun, H.M. et al. (2016). Magnetic nanofiber scaffold-induced stimulation of odontogenesis and pro-angiogenesis of human dental pulp cells through Wnt/MAPK/NF-κB pathways. Dent Mater. doi:10.1016/j.dental.2016.06.016.
¡¡ 19. Qiu, D. et al. (2016). Overexpression of FoxP1 is a novel biomarker of malignant human pancreatic cancer. Int J Clin Exp Med. 9:9054-9063.
¡¡ 20. Pekozer, G. G. et al. (2016). Influence of co-culture on osteogenesis and angiogenesis of bone marrow mesenchymal stem cells and aortic endothelial cells. Microvasc Res. doi:10.1016/j.mvr.2016.06.005.
¡¡ 21. Sun, Y. et al. (2016). Toll-like receptor 4 promotes angiogenesis in pancreatic cancer via PI3K/AKT signaling. Exp Cell Res. doi:10.1016/j.yexcr.2016.07.009.
¡¡ 22. Bid, H. K. et al. (2016). The Bromodomain BET inhibitor JQ1 suppresses tumor angiogenesis in models of childhood sarcoma. Mol Cancer Ther. doi:10.1158/1535-7163.
¡¡ 23. Yun, H. M. et al. (2016). Magnetic nanocomposite scaffolds combined with static magnetic field in the stimulation of osteoblastic differentiation and bone formation. Biomaterials. 85:88-98.
¡¡ 24. Chang, S. W. et al. (2016). Combined effects of mineral trioxide aggregate and human placental extract on rat pulp tissue and growth, differentiation and angiogenesis in human dental pulp cells. Acta Odontol Scand. doi:10.3109/00016357.2015.1120882.
¡¡ 25. Sun, T. et al. (2016). Forkhead box protein k1 recruits TET1 to act as a tumor suppressor and is associated with MRI detection. Jpn J Clin Oncol. doi:10.1093/jjco/hyv185.
¡¡ 26. Al-Mahrouki, A. A. et al. (2015). Ultrasound-stimulated microbubble enhancement of radiation treatments: endothelial cell function and mechanism.Oncoscience.2:944-957.
¡¡ 27. Chang, S. W. et al. (2015). Odontoblastic differentiation, inflammatory response, and angiogenic potential of 4 calcium silicate–based cements: MicroMega MTA, ProRoot MTA, Retro MTA, and experimental calcium silicate cement. J Endod. doi: 10.1016/j.joen.2015.04.018.
¡¡ 28. Zheng, D. et al. (2015). Silencing of miR-195 reduces diabetic cardiomyopathy in C57BL/6 mice. Diabetologia.  doi: 10.1007/s00125-015-3622-8.
¡¡ 29. Yamanegi, K. et al. (2015). Sodium valproate, a histone deacetylase inhibitor, modulates the vascular endothelial growth inhibitor-mediated cell death in human osteosarcoma and vascular endothelial cells. Int J Oncol. doi: 10.3892/ijo.2015.2924. 
¡¡ 30. Bae, W. J. et al. (2015). Effects of sodium tri-and hexametaphosphate on proliferation, differentiation, and angiogenic potential of human dental pulp cells. J Endod. doi: 10.1016/j.joen.2015.01.038.
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