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 제품안내  분자생물학   PCR Amplification  Real Time PCR

EvaGreen™ qPCR Dye

SYBR Green I을 대체해서 사용할수 있는 quantitative real-time PCR(qPCR)용 dye입니다.

제품 문의하기

  • EvaGreen은 기존에 셋팅된 조건의 변화없이 SYBR Green I을 대체하여 사용하실 수 있습니다.
  • "release-on-demand" DNA-결합 기술을 이용하여 PCR inhibition이 거의 없습니다.
  • SYBR® Green I보다 높은 감도로 형광이 매우 밝습니다.
  • 돌연변이를 일으키지 않으며, 세포독성이 없는 제퓸으로 별도의 분리수거 없이 처리가 간편합니다.
  • 보관 또는 PCR 사용시 열, 빛, 산-염기 조건에서도 매우 안정합니다.
  • Fast PCR과 Multiple PCR protocol에 사용 가능합니다.
    EvaGreen® dye binds to dsDNA via a "release-on-demand" mechanism.
    Figure 1. Comparison among Fast-Plus EvaGreen® master mix from Biotium and two fast SYBR® Green master mixes from two leading companies (company A and company Q) under similar condition. The inset is an enlarged view of the area near the baseline for better viewing the curve patterns of the much weaker signals of the two SYBR-based master mixes. Amplicon: ATPG fragment of human genomic DNA; instrument: ABI 7900 Fast.
    Figure 2. Comparison of cell membrane permeability between EvaGreen® dye and SYBR® Green I. HeLa cells were incubated with SYBR® Green I (1.2 μM) or EvaGreen® dye (1.2 μM) at 37 oC. Photographs were taken following incubation for 5 and 30 minutes. SYBR® Green I entered cells rapidly while EvaGreen® dye appeared membrane-impermeable as evident from the absence of cell nuclear staining. Image taken with long photo-exposure time revealed that EvaGreen® dye only associated with cell membranes.
    Figure 3. Excitation and emission spectra of EvaGreenTM in the presence of dsDNA in PBS buffer.
주문정보(Ordering Information)
31019EvaGreen® dye, 2000X in DMSO50uL
31000EvaGreen® dye, 20X in water5x1mL